A5010253445- Microbial metabolism and enzyme function
- Microbial Community Ecology and Physiology
- Anaerobic Digestion and Biogas Production
- Methane Hydrates and Related Phenomena
- Biofuel production and bioconversion
- Microbial bioremediation and biosurfactants
- Genomics and Phylogenetic Studies
- SARS-CoV-2 and COVID-19 Research
- Porphyrin Metabolism and Disorders
- Tuberculosis Research and Epidemiology
- Viral Infections and Outbreaks Research
- Infectious Encephalopathies and Encephalitis
- Diatoms and Algae Research
- COVID-19 diagnosis using AI
- Hemoglobin structure and function
- Alcohol Consumption and Health Effects
- Traditional Chinese Medicine Studies
- Biochemical Acid Research Studies
- Disaster Response and Management
- Animal Virus Infections Studies
- Bacteriophages and microbial interactions
- Ideological and Political Education
- Digital Imaging for Blood Diseases
- Yersinia bacterium, plague, ectoparasites research
- Global Public Health Policies and Epidemiology
Savitribai Phule Pune University
2018-2024
Agharkar Research Institute
2015-2024
BAIF Development Research Foundation
2022
University of Konstanz
2006-2011
University of Bremen
2011
Roots are the primary site of interaction between plants and microorganisms. To meet food demands in changing climates, improved yields stress resistance increasingly important, stimulating efforts to identify factors that affect plant productivity. The role bacterial endophytes reside inside remains largely unexplored, because analysis their specific functions is impeded by difficulties cultivating most prokaryotes. Here, we present first metagenomic approach analyze an endophytic community...
ABSTRACT The composition of diatom-associated bacterial communities was studied with 14 different unialgal xenic diatom cultures isolated from freshwater epilithic biofilms Lake Constance, Germany. A clear dominance Alphaproteobacteria observed, followed by Betaproteobacteria, Gammaproteobacteria, Bacteroidetes , and Verrucomicrobia . Pure the Cymbella microcephala which found to be dominant in were cocultivated six associated strains. All these strains able grow C. absence organic...
A novel methanotroph, strain LC 2 T , was isolated from the littoral sediment of Lake Constance by enrichment in opposing gradients methane and oxygen, followed traditional isolation methods. Strain grows on or methanol as its sole carbon energy source. It is a Gram-negative, non-motile, pale-pink-coloured methanotroph showing typical intracytoplasmic membranes arranged stacks. Cells are coccoid, elliptical rod-shaped occur often pairs. at low oxygen concentrations counter-gradients oxygen....
ABSTRACT The abundances and activities of aerobic methane-oxidizing bacteria (MOB) were compared in depth profiles littoral profundal sediments Lake Constance, Germany. Abundances determined by quantitative PCR (qPCR) targeting the pmoA gene fluorescence situ hybridization (FISH), data to methane oxidation rates calculated from high-resolution concentration profiles. qPCR using type I MOB-specific primers indicated that MOB represented a major proportion both at all depths. FISH sediments,...
With the COVID-19 pandemic reaching its worse heights, everyone is genuinely interested in origin of SARS-CoV-2. In our quest, we started with two important questions: First, were there any similar atypical pneumonia outbreaks, even on a smaller level, reported between SARS (2004) and (2019) from China? Secondly, where did beta-coronavirus most closely related to date SARS-CoV-2 at genome sequence strain RaTG13 (CoV4991) sampled horseshoe bat Yunnan province, exactly come from? It was found...
In sediments, methane-oxidizing bacteria live in opposing gradients of methane and oxygen. such a gradient system, the fluxes oxygen are controlled by diffusion consumption rates, rate-limiting substrate is maintained at minimum concentration layer consumption. Opposing were mimicked specific cultivation set-up which growth methanotrophic occurred as sharp band either c. 5 or 20 mm below air-exposed end. Two new strains isolated with this system. One isolate, strain LC 1, belonged to...
ABSTRACT Analysis of pmoA and 16S rRNA gene clone libraries methanotrophic bacteria in Lake Constance revealed an overall dominance type I methanotrophs both littoral profundal sediments. The sediments exhibited minor differences their community structures. Type X made up a significant part the only sediment were also found there as prominent peak by T-RFLP analyses.
Methanotrophs are aerobic to micro-aerophilic bacteria, which oxidize and utilize methane, the second most important greenhouse gas. The community structure of methanotrophs in rice fields worldwide has been studied mainly using culture-independent methods. Very few studies have focused on culturing from fields. We developed a unique method for cultivation field samples. Here, we used modified dilute nitrate mineral salts (dNMS) medium, with two cycles dilution till extinction series...
An alphaproteobacterium, strain Dia-1(T), was isolated from algae-dominated biofilms on stones the littoral zone of Lake Constance, Germany. This bacterium after initial enrichment in spent medium obtained growth a diatom culture. Numerous sugars and some organic acids alcohols served as substrates. The grew slowly, strictly aerobic but microaerophilic, did not grow cultures shaken under air. 16S rRNA gene sequence analysis indicated that Dia-1(T) distantly related to representatives genera...
Enteric fermentation of methane by ruminant animals represents a major source anthropogenic methane. Significantly less information is available on the existence methanotrophs in gut ruminants. Therefore, detailed strain descriptions isolated from faeces or are rare. We present first report enrichment and isolation methanotroph, BlB1, an Indian antelope (blackbuck). The 16S rRNA gene sequence BlB1 showed highest identity (98.40% identity) to Methylobacter marinus A45T luteus NCIMB 11914T....
Rice fields are one of the important anthropogenic sources methane emissions. Methanotrophs dwelling near rice roots and at oxic–anoxic interface paddy can oxidize a large fraction generated therefore considered to be important. Nitrogen fixation in root-associated methanotrophs is well known. Our aim this study was explore potential as bio-inoculants for studies were performed pot experiments monsoon. Ten indigenously isolated used belonging eight diverse genera Type Ia, Ib, II...
Genomic analysis indicates that SARS-CoV-2 is most related to RaTG13, a beta corona virus derived from bats by 96% 1, study led Dr. Zhengli Shi. The physical description of RaTG13 not available and at present only genome on the public database. Since one main supports for SARS-COV-2 have natural origin, it utmost importance understand origin this virus/ exact sample which was sequenced. Here we tried investigate would been sequenced its phylogenetic relationship previously described bat...
Rice fields are one of the important sources anthropogenic methane. Methanotrophs can oxidize up to 30 % produced methane and thus have a pivotal environmental role in mitigation. India occupies largest region under rice cultivation; however, most studies done on methanotrophic communities focused Northern region. We studied community flooded, organically fertilized field using pmoA clone library approach. Organic impose more serious threat as they produce gene is main functional which...