Abstract: Perlecan is a specific heparan sulfate proteoglycan that accumulates in the fibrillar β‐amyloid (Aβ) deposits of Alzheimer's disease. purified from Engelbreth‐Holm‐Swarm tumor was used to define perlecan's interactions with Aβ and its effects on fibril formation. Using solid‐phase binding immunoassay, freshly solubilized full‐length peptides bound immobilized perlecan at two sites, representing both high‐affinity [ K D = ∼5.8 × 10 −11 M for (1–40); ∼6.5 −12 (1–42)] lower‐affinity...

Abstract: Our previous studies have demonstrated that perlecan and perlecan‐derived glycosaminoglycans (GAGs) not only bind β‐amyloid protein (Aβ) 1‐40 1‐42, but are also potent enhancers of Aβ fibril formation stabilize amyloid fibrils once formed. However, it was determined which moieties in heparan sulfate GAG chains may be responsible for the observed effects whether other GAGs were capable a similar enhancement as with GAGs. In present study, thioflavin T fluorometry (over 1‐week...

Islet amyloidosis is characterized by the deposition and accumulation of amylin in pancreatic beta-cells observed 90% patients with type 2 diabetes. Previous studies have also revealed presence specific heparan sulfate proteoglycan, perlecan, colocalized to islet amyloid deposits, similar perlecan's known involvement other proteins. In present study, perlecan purified from Engelbreth-Holm-Swarm (EHS) tumor was used define interactions (i.e., polypeptide) its effects on fibril formation....

Abstract Brain aging and Alzheimer’s disease both demonstrate the accumulation of beta-amyloid protein containing “plaques” tau “tangles” that contribute to accelerated memory loss cognitive decline. In present investigation we identified a specific plant extract its constituents as potential alternative natural solution for preventing reducing brain “plaques tangles”. PTI-00703 cat’s claw ( Uncaria tomentosa from Peruvian source), Amazon rain forest, was potent inhibitor reducer fibrils...

To evaluate the role of transvaginal power Doppler sonography to discriminate between benign and malignant endometrial conditions in women presenting with postmenopausal bleeding thickened endometrium at baseline sonography.Ninety-one (median age, 58 years; range, 47-83 years) uterine a (> or = 5-mm double-layer thickness) on were included this prospective study. Endometrial blood flow distribution was assessed all patients by immediately after B-mode sonography. Three different vascular...

Abstract Background The mitroaortic intervalvular fibrosa is an avascular structure near the left ventricular outflow tract, between mitral and aortic valves. Mitroaortic complications, such as tamponade, hemopericardium, abscesses, are rare often diagnosed postmortem. On other hand, COVID-19 pandemic notably impacted pediatric patients with congenital heart diseases, who frequently presented cardiac complications including arrhythmias, elevated troponins, myocarditis, failure. However,...

Las fibras de celulosa naturales presentan propiedades ideales para el desarrollo materiales innovadores, por su sostenibilidad, renovabilidad y bajo costo producción. Este estudio tiene como objetivo obtener a partir del líber la planta cáñamo industrial mediante hidrólisis alcalina oxidativa seguida ácida. Se determinaron las condiciones óptimas maximizar contenido alfa-celulosa caracterización química. La biomasa se sometió un proceso descripción física, análisis inmediato pretratamiento...

BACKGROUND: The objective was to assess whether expectant management of sonographically benign ovarian cysts may be an option for selected asymptomatic premenopausal women. METHODS: This is a prospective observational longitudinal study. Between January 1997 and December 2002, 323 women (mean age: 40.6 years; range: 19–50 years) diagnosed as having cyst measuring <6 cm were offered conservative with periodic follow-up at 6–12 month intervals. In all cases, first check performed 3 months...

The aim of this work was to achieve a better understanding the bacterial pathogens associated with stillbirths that would serve inform clinical interventions directed at reducing adverse pregnancy outcome. A prospective observational study conducted participation 22 women from northern Peru, whom 11 experienced fetal death in utero and delivered preterm births. Swabs were taken vagina, placenta, amniotic fluid axilla infant birth by Caesarean section. populations vagina space each...

β-Amyloid protein (Aβ) is a major component of neuritic plaques and cerebrovascular amyloid deposits in the brains patients with Alzheimer's disease (AD). Inhibitors Aβ fibrillogenesis are currently sought as potential future therapeutics for AD related disorders. In present study, basement membrane laminin was found to bind 1–40 single dissociation constant, Kd = 2.7 × 10–9 M, serve potent inhibitor fibril formation. 25 μM incubated at 37°C 1 week presence 100 nM or other components,...

Two chondroitin sulfate‐containing complexes have been isolated from fetal bovine serum and shown to contain the serine protease inhibitor bikunin. A complex of 126 kDa contains bikunin linked by a sulfate chain protein with homology HC2 component human inter‐α‐trypsin inhibitor. This represents extracellular matrix stabilizing factor recently described as bikunin‐containing fraction necessary for expansion cumulus [(1992) J. Biol. Chem. 267, 12380‐12386]. second 236 contains, in addition...

Initially developed in 1992 as an MR imaging agent, the family of protected graft copolymers (PGC) is based on a conjugate polylysine backbone to which methoxypoly(ethylene glycol) (MPEG) chains are covalently linked random fasion via N-ε-amino groups. While PGC relatively simple terms its chemcial composition and structure, it has proved be versatile platform for vivo drug delivery. The advantages poly amino acid grafting include multiple available linking sites adaptor molecules. PEG does...

The origin of the heparan sulfate proteoglycan (PG), perlecan, in beta-amyloid protein (Aβ)-containing amyloid deposits Alzheimer's disease (AD) brain is not known. In present investigation we used indirect immunofluorescence, SDS-PAGE, and Western blotting with a specific perlecan core antibody to identify possible cell candidates production both primary cultures rat infusion model. Double triple-labeled immunofluorescence was performed on dissociated septal using antibodies for...

This study was conducted to evaluate the potential role of transvaginal color Doppler sonography (TVCD) in predicting response concurrent chemoradiotherapy for locally advanced cervical cancer.Tumor vascularity assessed using TVCD before start 21 patients (median age, 47 years; range, 31-75 years) with histologically proven cancer. The lowest resistance index (RI), pulsatility (PI), and highest peak systolic velocity (PSV) from central vessels within tumor were recorded used analysis. All...

The objective of this study was to evaluate whether intratumoral blood flow as assessed by transvaginal color Doppler ultrasonography (TVCD) correlates with some tumor features in cervical cancer. Clinical, sonographic, and histologic data on 49 women (mean age: 50.3 years, range: 25-85 years) diagnosed having a carcinoma from the uterine cervix were reviewed. Intratumoral TVCD all cases. Subjective impression amount (scanty, moderate, or abundant) well lowest resistance index (RI) highest...

The selective history of a population can influence its subsequent evolution, an effect known as historical contingency. We previously observed that five six replicate populations were evolved in glucose-limited environment for 2000 generations, then switched to lactose 1000 had higher fitness increases than started directly from the ancestor. To test if selection glucose systematically increased evolvability, we 12 replay populations--six subsample and single randomly selected clone--from...

Cirrhosis is a liver disease that leads to increased intrahepatic resistance, portal hypertension (PH), and splanchnic hyperemia resulting in ascites, variceal bleeding, hepatorenal syndrome. Terlipressin, prodrug converts short half-life vasopressin receptor 1 A (V1a) full agonist [8-Lys]-Vasopressin (LVP), an intravenous treatment for PH complications, but hyponatremia ischemic side effects require close monitoring. We developed PHIN-214 which into PHIN-156, more biologically stable V1a...

Targeting the initial formation of amyloid assemblies is a preferred approach to therapeutic intervention in amyloidoses, which include such diseases as Alzheimers, Parkinsons, Huntingtons, etc., early-stage, oligomers that form before development β-conformation-rich fibers are thought be toxic. X-ray patterns from always show two common intensity maxima: one at 4.7 Å corresponding hydrogen-bonding spacing between β-chains, and other ∼10 β-pleated sheets. We report here application fiber...

Abstract Background Vascular parameters, such as vascular volume, flow, and permeability, are important disease biomarkers for both type 1 2 diabetes. Therefore, it is essential to develop approaches monitor the changes in pancreatic microvasculature non‐invasively. Methods Here, we describe application of long‐circulating, paramagnetic T1 contrast agent, protected Graft Copolymer bearing covalently linked gadolinium diethylenetriaminepentaacetic acid residues labelled with fluorescein...

Co-infusion of the specfic heparan sulfate proteoglycan (HSPG), perlecan, and beta-amyloid protein (Aβ) into rodent hippocampus leads to a consistent animal model study effects fibrillar Aβ amyloid in brain [Snow, A.D. et al. (1994) Neuron 12,219-2341. In present study, we describe our rapid novel method perlecan isolation. The isolation does not require cesium chloride centrifugation exploits newly discovered aggregating property ˜220 kDa PG observed during gel filtration chromatography,...

6 children with idiopathic nephrotic syndrome were investigated during clinical relapse to examine the interrelation between distal urinary acidification and sodium excretion. Blood urine studies initiated 4 h after completion of ammonium chloride loading, prior following intravenous administration furosemide. Values for plasma bicarbonate before furosemide not significantly different. In control periods, when excretion was very low, a defect in demonstrated (UPH: 6.09 +/- (SD) 0.27; UTAV...