A5005818304- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- RNA modifications and cancer
- PARP inhibition in cancer therapy
- Sirtuins and Resveratrol in Medicine
- CRISPR and Genetic Engineering
- Circular RNAs in diseases
- Epigenetics and DNA Methylation
- DNA Repair Mechanisms
- Cytomegalovirus and herpesvirus research
- BRCA gene mutations in cancer
- RNA regulation and disease
- melanin and skin pigmentation
- HIV/AIDS Research and Interventions
- MicroRNA in disease regulation
- HIV Research and Treatment
- Genomics and Chromatin Dynamics
Hospital Sírio-Libanês
2017-2023
DASA (Brazil)
2023
Genomic (Brazil)
2023
Universidade Federal de São Paulo
2011-2017
Abstract Background RNA-binding proteins (RBPs) function as master regulators of gene expression. Alterations in RBP expression and are often observed cancer influence critical pathways implicated tumor initiation growth. Identification characterization oncogenic RBPs their regulatory networks provide new opportunities for targeted therapy. Results We identify the protein SERBP1 a novel regulator glioblastoma (GBM) development. High is prevalent GBMs correlates with poor patient survival...
Melanoma progression requires deregulation of gene expression by currently uncharacterized epigenetic mechanisms. A mouse model based on changes in cell microenvironment was developed our group to study melanocyte malignant transformation. lines (4C11- and 4C11+) were obtained as result 5 sequential anchorage blockades non-tumorigenic melan-a melanocytes. Melan-a cells submitted 4 de-adhesion cycles also established (4C), are represent an intermediary phase tumor progression. The aim this...
Epigenetic modifications refer to a number of biological processes which alter the structure chromatin and its transcriptional activity such as DNA methylation histone post-translational processing. Studies have tried elucidate how viral genome products are affected by epigenetic imposed cell machinery it affects ability virus either, replicate produce viable progeny or be driven latency. The purpose this study was evaluate in PBMCs CD4+ cells after HIV-1 infection analyzing three...
// Fabiana M. Meliso 1, * , Danilo Micali Camila T. Silva 1 Thaís S. Sabedot 2 Simon G. Coetzee Adrian Koch 3 Fabian B. Fahlbusch 4 Houtan Noushmehr Regine Schneider-Stock and Miriam Jasiulionis Ontogeny Epigenetics Laboratory, Pharmacology Department, Universidade Federal de São Paulo - UNIFESP, Paulo, SP, Brazil Department of Genetics, Ribeirão Preto Medical School, University Preto, Experimental Tumorpathology, Institute Pathology, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU),...
The exon junction complex (EJC) plays key roles throughout the lifespan of RNA and is particularly relevant in nervous system. We investigated two EJC members, paralogs MAGOH MAGOHB, with respect to brain tumour development. High MAGOH/MAGOHB expression was observed 14 types; glioblastoma (GBM) showed greatest difference compared normal tissue. Increased associated poor prognosis glioma patients, while knockdown affected different cancer phenotypes. Reduced GBM cells caused alterations...
Homologous recombination deficiency (HRD) has become an important prognostic and predictive biomarker for patients with high-grade serous ovarian cancer who may benefit from poly-ADP ribose polymerase inhibitors (PARPi) platinum-based therapies. HRD testing provides relevant information to personalize patients' treatment options been progressively incorporated into diagnostic laboratories. Here, we assessed the performance of in-house system deployable in a clinical setting, comparing...
ABSTRACT Homologous Recombination Repair (HRR) testing has become increasingly important in clinical genomic labs due to the use of poly-ADP-ribose polymerase (PARP) inhibitor therapy for epithelial ovarian, fallopian tube, or peritoneum cancer. While sequencing and copy number variation analysis can identify patients with a pathogenic mutation BRCA1 BRCA2 who benefit from PARPi therapy, there are also may but do not have these mutations. To address this, our lab developed test called...
Homologous recombination deficiency (HRD) has become an important prognostic and predictive biomarker for patients with high-grade serous ovarian cancer who may benefit from poly-ADP ribose polymerase inhibitors (PARPi) platinum-based therapies. HRD testing provides relevant information to personalize patients’ treatment options, it been progressively incorporated in diagnostic laboratories. In this study, we evaluated the feasibility of in-house deployable a clinical setting,...
Homologous recombination deficiency (HRD) has become an important prognostic and predictive biomarker for patients with high-grade serous ovarian cancer who may benefit from poly-ADP ribose polymerase inhibitors (PARPi) platinum-based therapies. HRD testing provides relevant information to personalize patients’ treatment options, it been progressively incorporated in diagnostic laboratories. In this study, we evaluated the feasibility of in-house deployable a clinical setting,...
Abstract Melanoma is one of the most worrying tumors due to its high capacity cause metastasis. Sirt1, a class III histone deacetylase, was shown be attracted sites double strand breaks in DNA, favoring, by recruiting components epigenetic machinery, aberrant silencing that region (O’HAGAN et al., 2008). Beyond that, subsequent studies have attributed important roles Sirt1 and other such as DNA methyltransferases (such Dnmt3b), tumor initiation progression. In this sense, using murine model...
Abstract miRNAs are non-coding RNAs that bind to mRNA targets preventing their translation. It is predicted over 30% of mRNAs regulated by miRNAs. Therefore these molecules considered essential many biological responses, such as cell proliferation, apoptosis and stress responsiveness. Loss or gain function contributes development diseases, included cancer. Melanoma rises from malignant transformation melanocytes. Despite being rare, it responsible for the greatest number deaths promoted all...
Abstract In a murine melanoma model of malignant transformation promoted by sustained stress, increased levels reactive oxygen species were found to result in DNA damage and related massive epigenetic alterations. Since the chromatin modifier Sirtuin-1 (SIRT1) is protein attracted double-stranded break (DSB) sites can recruit other components machinery, we aimed define role SIRT1 melanomagenesis through our model. The marker, γH2AX was melanocytes after 24 hours deadhesion, accompanied...
Abstract According to the World Health Organization, glioblastoma multiforme (GBM) represents 15-20% of all primary intracranial tumors and it is one most worrisome types tumor, due its high aggressiveness with low response treatments. Currently, patients diagnosed GBM survive less than 15 months after diagnosis. Thus, there a growing interest in study molecular signatures that contribute more effective therapy. Given prevalence defects splicing mechanisms found cancer, small modulating...