A5067931521- Cell death mechanisms and regulation
- Genomics, phytochemicals, and oxidative stress
- Osteoarthritis Treatment and Mechanisms
- NF-κB Signaling Pathways
- Cytokine Signaling Pathways and Interactions
- Immune Response and Inflammation
- Protein Kinase Regulation and GTPase Signaling
- Phagocytosis and Immune Regulation
- Lipid Membrane Structure and Behavior
- Animal Virus Infections Studies
- Fungal Plant Pathogen Control
- Natural product bioactivities and synthesis
- SARS-CoV-2 and COVID-19 Research
- Cellular transport and secretion
- Inflammatory mediators and NSAID effects
- Exercise and Physiological Responses
- vaccines and immunoinformatics approaches
- Melanoma and MAPK Pathways
- COVID-19 Clinical Research Studies
- Adenosine and Purinergic Signaling
- Medicinal Plant Research
- Venomous Animal Envenomation and Studies
- Plant Virus Research Studies
- Asthma and respiratory diseases
- Dupuytren's Contracture and Treatments
University of East Anglia
2010-2022
University of Edinburgh
2003-2010
University of Aberdeen
1999-2003
Multiple synaptic vesicle (SV) retrieval modes exist in central nerve terminals to maintain a continual supply of SVs for neurotransmission. Two such are clathrin-mediated endocytosis (CME), which is dominant during mild neuronal activity, and activity-dependent bulk (ADBE), intense activity. However, little known about how activation these SV impact the replenishment total recycling pool pools that reside within it, readily releasable (RRP) reserve pool. To address this question, we...
Sulforaphane (SFN) has been reported to regulate signaling pathways relevant chronic diseases. The aim of this study was investigate the impact SFN treatment on in chondrocytes and determine whether sulforaphane could block cartilage destruction osteoarthritis.Gene expression, histone acetylation, transcription factors NF-E2-related factor 2 (Nrf2) NF-κB were examined vitro. bovine nasal explant model destabilization medial meniscus (DMM) osteoarthritis mouse used assess chondroprotection at...
The TF-1 human erythroleukemic cell line exhibits opposing physiological responses toward tumor necrosis factor-alpha (TNF) treatment, dependent upon the mitotic state of cells. Mitotically active cells in log growth respond to TNF by rapidly undergoing apoptosis whereas exposure stimulates cellular proliferation mitotically quiescent concentration-dependent TNF-induced was monitored metabolic activity and confirmed both DNA epifluorescence fragmentation. Moreover, these could be detected...
The pleitropic actions of tumour necrosis factor-α (TNF) are transmitted by the type I 55kDa TNF receptor (TNFR1) and II 75kDa (TNFR2), but signalling mechanisms elicited these two receptors not fully understood. In present study, we report for first time subtype-specific differential kinase activation in cell models that respond to undergoing apoptotic death. KYM-1human rhabdomyosarcoma cells HeLa human cervical epithelial cells, engineered overexpress TNFR2, displayed c-Jun N-terminal...
Osteoarthritis is a major cause of disability and there no current pharmaceutical treatment which can prevent the disease or slow its progression. Dietary advice supplementation clearly an attractive option since it has low toxicity ease implementation on population level. We have previously demonstrated that sulforaphane, dietary isothiocyanate derived from glucosinolate precursor found in broccoli, cartilage destruction cells, vitro vivo models osteoarthritis. As next phase this research,...
The COVID-19 pandemic continues to expand globally, with case numbers rising in many areas of the world, including Eastern Mediterranean Region. Lebanon experienced its largest wave infections from January April 2021. Limited genomic surveillance was undertaken, just 26 SARS-CoV-2 genomes available for this period, nine which were travellers detected by other countries. Additional genome sequencing is thus needed allow variants circulation. In total, 905 sequenced using ARTIC protocol....
The hormonally regulated Ca(2+)-dependent enzyme, cytosolic phospholipase A(2) (cPLA(2)) is activated by a range of inflammatory stimuli. Tumour necrosis factor-alpha (TNF) one the first known stimuli for cPLA(2) but it not whether both TNF receptor subtypes are involved in activating lipase. In present study, we show time that type I 55 kDa TNFR (TNFR1) and II 75 (TNFR2) stimulate with distinct signalling mechanisms. TNFR1 activates mitogen-activated protein kinase (MAPK) p38MAPK. then...
The pleitropic actions of tumour necrosis factor-α (TNF) are transmitted by the type I 55kDa TNF receptor (TNFR1) and II 75kDa (TNFR2), but signalling mechanisms elicited these two receptors not fully understood. In present study, we report for first time subtype-specific differential kinase activation in cell models that respond to undergoing apoptotic death. KYM-1human rhabdomyosarcoma cells HeLa human cervical epithelial cells, engineered overexpress TNFR2, displayed c-Jun N-terminal...
Abstract Background The COVID-19 pandemic continues to expand globally, with case numbers rising in many areas of the world, including Eastern Mediterranean Region. Lebanon experienced its largest wave infections from January April 2021. Limited genomic surveillance was undertaken, just twenty six SARS-CoV-2 genomes available for this period, nine which were travellers detected by other countries. Additional genome sequencing is thus needed allow variants circulation. Methods Nine hundred...
The activation of the extracellular signal-regulated kinases (ERKs) by tumour necrosis factor-alpha (TNF) receptors (TNFRs) is an integral part cytokine's pleiotropic cellular responses. Here we report differences in caspase sensitivity and TNFR subtype members ERK family. Inhibition HeLa cells function pharmacological inhibitors or expression CrmA (cytokine response modifier A), a viral protein, blocks TNF-induced apoptosis caspase-dependent protein kinase Cdelta poly(ADP-ribose) polymerase...
Abstract Osteoarthritis ( OA ) is a degenerative joint disease characterised in part by destruction of articular cartilage. There are currently no disease‐modifying drugs to treat , with replacement the only treatment offered patients at end‐stage disease. With age major risk factor for number predicted double 2030. An understanding role bioactive molecules from habitual diet on health offers novel way which prevent onset or slow progression . Our research has indicated that sulforaphane SFN...
Phase 2 of the Norwich COVID-19 testing initiative: an evaluationUniversity campuses have experienced widespread transmission severe acute respiratory syndrome coronavirus (SARS-CoV-2).The virus is particularly prevalent in student-age population 1 and more likely to be asymptomatic. 2he Testing Initiative phase (NTI2) was asymptomatic disease 2019 (COVID-19) polymerase chain reaction (PCR) programme introduced at University East Anglia campus between September December 2020.NTI2 followed a...
Conference Abstract| February 01 2000 Unaltered intracellular calcium regulation in tumour necrosis factor receptor-mediated apoptotic cell death Shona M. McFarlane; McFarlane 1Dept of Biomedical Sciences, Institute Medical University Aberdeen, AB25 2ZD, United Kingdom. Search for other works by this author on: This Site PubMed Google Scholar Helen Anderson; Anderson Steven J. Tucker; Tucker Orla Jupp; Jupp David MacEwan Author and article information Publisher: Portland Press Ltd Online...
<h3>Introduction and Objectives</h3> Metalloproteinases are implicated in the development of airway remodelling asthma due to their ability cleave collagen elastin with extracellular matrix. We optimised a method purify messenger Ribonucleic acid (mRNA) sputum samples. <h3>Methods</h3> The mRNA expression wide range pertinent Matrix (MMP), A Disintegrin And (ADAM), Metalloproteinase Thrombospondin Motifs (ADAMTS) Tissue Inhibitors (TIMP) was measured from induced hypertonic saline using...