A5087945181- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Escherichia coli research studies
- Pneumonia and Respiratory Infections
- vaccines and immunoinformatics approaches
- SARS-CoV-2 and COVID-19 Research
- Bacterial Infections and Vaccines
- Immunotherapy and Immune Responses
- Bacteriophages and microbial interactions
- Pharmaceutical and Antibiotic Environmental Impacts
- Antimicrobial Peptides and Activities
- Antibiotic Use and Resistance
- Bacterial biofilms and quorum sensing
- Virus-based gene therapy research
- Bacterial Identification and Susceptibility Testing
- Antibiotics Pharmacokinetics and Efficacy
- Bacillus and Francisella bacterial research
- Antimicrobial Resistance in Staphylococcus
- Viral gastroenteritis research and epidemiology
- Immune Response and Inflammation
- Cytomegalovirus and herpesvirus research
- Enterobacteriaceae and Cronobacter Research
- HIV Research and Treatment
- Herpesvirus Infections and Treatments
- Streptococcal Infections and Treatments
University of Notre Dame
2017-2025
Victoria University of Wellington
2024
Malaghan Institute of Medical Research
2024
Instituto de Salud Carlos III
2013-2023
Centro Nacional de Microbiologia
2022
Instituto de Biomedicina de Sevilla
2010-2018
Universidad de Sevilla
2010-2018
Hospital Universitario Virgen del Rocío
2008-2016
Red Espanola de Investigacion en Patologia Infecciosa
2013-2014
University of Pittsburgh
2012
Acinetobacter baumannii , which causes serious infections in immunocompromised patients, expresses high-affinity iron acquisition functions needed for growth under iron-limiting laboratory conditions. In this study, we determined that the initial interaction of ATCC 19606 T type strain with A549 human alveolar epithelial cells is independent production BasD and BauA, proteins acinetobactin biosynthesis transport, respectively. contrast, these are required to persist within cause their...
To investigate the molecular epidemiology, antimicrobial susceptibility and carbapenem resistance determinants of Acinetobacter baumannii isolates from respiratory tract samples patients diagnosed with ventilator-associated pneumonia (VAP) who were enrolled in MagicBullet clinical trial. A. prospectively cultured 65 15 hospitals Greece, Italy Spain. Susceptibility testing was performed by broth microdilution. Carbapenem identified PCR sequencing. Molecular epidemiology investigated using...
Acinetobacter baumannii (American Type Culture Collection strain 19606) acquires mutations in the pmrB gene during vitro development of resistance to colistin. The colistin-resistant has lower affinity for colistin, reduced vivo fitness (competition index, .016), and decreased virulence, both terms mortality (0% lethal dose, 6.9 vs 4.9 log colony-forming units) survival a mouse model peritoneal sepsis. These results may explain low incidence dissemination colistin A. clinical settings.
ABSTRACT Acinetobacter baumannii causes pneumonias, bacteremias, and skin soft tissue infections, primarily in the hospitalized setting. The incidence of infections caused by A. has increased dramatically over last 30 years, while at same time treatment these been complicated emergence antibiotic-resistant strains. Despite trends, no vaccines or antibody-based therapies have developed for prevention infection. In this study, an outer membrane complex vaccine consisting multiple surface...
Adhesion to host cells is an initial and important step in Acinetobacter baumannii pathogenesis. However, there relatively little information on the mechanisms by which A. binds interacts with cells. Adherence extracellular matrix proteins, such as fibronectin, affords pathogens a mechanism invade epithelial Here, we found that adheres more avidly immobilized fibronectin than control protein. Free used competitor resulted dose-dependent decreased binding of fibronectin. Three outer membrane...
The fitness and virulence costs associated with the clinical acquisition of colistin resistance by Acinetobacter baumannii were evaluated. growth strain CR17 (colistin resistant) was less than that CS01 susceptible) when strains grown in competition (72-h index, 0.008). In a murine sepsis model, reached spleen concentrations coinfecting 9.31 6.97 log10 CFU/g, respectively, an vivo index 0.016. Moreover, more virulent respect to mortality time death.
Acinetobacter baumannii can acquire resistance to colistin via complete loss of lipopolysaccharide (LPS) biosynthesis due mutations in the lpxA, lpxC and lpxD genes. However, although is increasingly being used for treatment multidrug resistant infections, very few A. clinical isolates develop through LPS biosynthesis. This may suggest that affects virulence traits play a role transmission pathogenesis baumannii. In this study we characterize multiple phenotypes resistant, LPS-deficient...
Accurate detection of vancomycin-resistant enterococci (VRE) is essential in preventing transmission health care settings. Chromogenic media are widely used for screening VRE because fast turnaround times (TAT) and high sensitivity. We report an outbreak Enterococcus faecium bearing vanA yet susceptible to vancomycin (vancomycin-variable [VVE]). Between October 2009 March 2011, clinical specimens (n=14,747) were screened using VRE-selective medium and/or PCR. VVE isolates genotyped determine...
The increasing clinical importance of infections caused by multidrug resistant Acinetobacter baumannii warrants the development novel approaches for prevention and treatment. In this context, vaccination certain patient populations may contribute to reducing morbidity mortality pathogen. Vaccines against Gram-negative bacteria based on inactivated bacterial cells are highly immunogenic have been shown produce protective immunity a number species. However, high endotoxin levels present in...
LpxC inhibitors have generally shown poor in vitro activity against Acinetobacter baumannii We show that the inhibitor PF-5081090 inhibits lipid A biosynthesis, as determined by silver staining and measurements of endotoxin levels, significantly increases cell permeability. The presence at 32 mg/liter increased susceptibility to rifampin, vancomycin, azithromycin, imipenem, amikacin but had no effect on ciprofloxacin tigecycline. Potentiating existing antibiotics with may represent an...