A5023091252- Fungal and yeast genetics research
- RNA and protein synthesis mechanisms
- Mitochondrial Function and Pathology
- RNA Research and Splicing
- RNA modifications and cancer
- Pain Mechanisms and Treatments
- Bacterial Genetics and Biotechnology
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Microbial metabolism and enzyme function
- Neuroscience and Neural Engineering
- Botulinum Toxin and Related Neurological Disorders
- ATP Synthase and ATPases Research
- Peripheral Neuropathies and Disorders
- RNA regulation and disease
- Nerve injury and regeneration
- 14-3-3 protein interactions
- Cancer Treatment and Pharmacology
- Trypanosoma species research and implications
- Glycosylation and Glycoproteins Research
- Genetic Neurodegenerative Diseases
- Protist diversity and phylogeny
- Muscle activation and electromyography studies
- Neurological and metabolic disorders
- Microbial Natural Products and Biosynthesis
Centre National de la Recherche Scientifique
2002-2025
Institut de Biologie Physico-Chimique
2020-2025
Sorbonne Université
2002-2025
Laboratoire de Biologie Moléculaire et Cellulaire des Eucaryotes
2024
Université Claude Bernard Lyon 1
2024
Institut de Biologie Intégrative de la Cellule
2016-2021
CEA Paris-Saclay
2018-2021
Université Paris-Saclay
2018-2021
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2018-2021
Université Paris-Sud
1997-2018
In Saccharomyces cerevisiae the nicotinic acid moiety of NAD + can be synthesized from tryptophan using kynurenine pathway or incorporated directly nicotinate phosphoribosyl transferase ( NPT1 ). We have identified genes that encode enzymes and for BNA5 (YLR231c) BNA6 (YFR047c) confirmed they kynureninase quinolinate respectively. show deletion encoding are co‐lethal with Δ npt1 , demonstrating no other synthesis exists in S. . Also, we under anaerobic conditions is a auxotroph.
Carbon catabolite repression is mediated in Aspergillus nidulans by the negative acting protein CreA. The CreA repressor plays a major role control of expression alc regulon, encoding proteins required for ethanol utilization pathway. It represses directly, at transcriptional level, specific transacting gene alcR, two structural genes alcA and aldA, other all physiological growth conditions. Among seven putative sites identified alcApromoter region, we have determined functional targets AlcR...
Expression of the structural genes for alcohol and aldehyde dehydrogenase, alcA aldA, respectively, enables fungus Aspergillus nidulans to grow on ethanol. The pathway-specific transcriptional activator AlcR mediates induction ethanol catabolism in presence a coinducing compound. Ethanol is further subject negative control mediated by general carbon catabolite repressor CreA. Here we show that, contrast alcR, aldA gene not directly CreA repression. A single cis-acting element activation...
Abstract The copper ionophore disulfiram (DSF) is commonly used to treat chronic alcoholism and has potential anti-cancer activity. Using a yeast-based screening assay of FDA-approved compounds, DSF was herein identified for its ability improve oxidative phosphorylation-dependent growth various yeast models mitochondrial diseases caused by wide range defects in ATP synthase, complexes III IV, cardiolipin remodeling, maintenance translation the genome. This compound also showed beneficial...
The alcA gene which is part of the recently identified ethanol regulon, one most strongly inducible genes in Aspergillus nidulans. Its transcriptional activation mediated by AlcR transactivator contains a DNA-binding domain belonging to C6 zinc binuclear cluster family. differs from other members this family several features, striking characteristic being its binding both symmetric and asymmetric DNA sites with same apparent affinity. However, also able bind single site high affinity,...
Mitochondrial mRNAs encode key subunits of the oxidative phosphorylation complexes that produce energy for cell. In Saccharomyces cerevisiae, mitochondrial translation is under control translational activators, specific to each mRNA. Schizosaccharomyces pombe, which more closely resembles human system by its DNA structure and physiology, most activators appear be either lacking, or recruited post-translational functions. By combining bioinformatics, genetic biochemical approaches we...
In the A. nidulans ethanol utilization pathway, specific induction is mediated by transactivator AlcR which subject to strong positive autogenous regulation and activates transcription of two structural genes alcA aldA . Carbon catabolite repression CreA represses directly transacting gene alcR genes. We show here that basal expression also controlled regulatory circuits, positively negatively repressor CreA, being only control repressor.
Background information . The CBK1 gene of Saccharomyces cerevisiae encodes a protein kinase that is member the NDR (nuclear Dbf2‐related) family kinases, which are involved in morphogenesis and cell proliferation. Previous studies have shown deletion leads to loss polarity formation large aggregates cells. This aggregation phenotype due daughter cell‐specific accumulation transcription factor Ace2p, responsible for genes whose products necessary final separation mother at end division....
Mitochondria have their own translation machinery that produces key subunits of the OXPHOS complexes. This relies on coordinated action nuclear-encoded factors bacterial origin are well conserved between humans and yeast. In humans, mutations in these can cause diseases; yeast, abolishing mitochondrial destabilize DNA. We show when genome contains no introns, loss yeast Mif3 Rrf1 involved ribosome recycling neither blocks nor destabilizes Rather, absence increases synthesis...
Abstract In Saccharomyces cerevisiae the protein kinase Cbk1p is a member of regulation Ace2p and cellular morphogenesis (RAM) network that involved in cell separation after cytokinesis, integrity, polarity. separation, RAM promotes daughter cell-specific localization transcription factor Ace2p, resulting asymmetric genes whose products are necessary to digest septum joining mother cell. SSD1 play role maintenance integrity. presence wild-type gene, deletion any component causes lysis. We...
Abnormalities in axonal transport of proteins are thought to play an important role the pathogenesis diabetic neuropathy. Gangliosides exert a positive action on numerous alterations biochemistry and physiology nerves. This study was undertaken assess effects exogenous gangliosides structural such as actin tubulin sensory fibers short-term (9-wk) long-term (6-mo) rats. Adult Sprague-Dawley rats were made with single injection 70 mg/kg streptozocin i.p. Subgroups injected daily either highly...
The Oxa1/YidC/Alb3 family plays a key role in the biogenesis of respiratory and photosynthetic complexes bacteria organelles. In Saccharomyces cerevisiae, Oxa1 mediates co-translational insertion mitochondrially encoded subunits three III, IV V within inner membrane also controls late step complex assembly. No crystal structure YidC or is available little known about respective each transmembrane segment (TM) hydrophilic loop this polytopic protein on complexes. Here, we have generated...
The mitochondrial genomes of Saccharomyces cerevisiae strains contain up to 13 introns. An intronless recombinant genome introduced into the nuclear background S. strain W303 gave CW252 strain, which is used model respiratory pathologies. complete sequence this was obtained using a hybrid assembling methodology.
ABSTRACT Cytoplasmic RNA granules compartmentalize phases of the translation cycle in eukaryotes. We previously reported localization oxidized to cytoplasmic foci called bodies (ORBs) human cells. show here that ORBs are Saccharomyces cerevisiae. Several lines evidence support a role for compartmentalization no-go decay and ribosome quality control, control pathways recognize clear aberrant mRNAs, including those with bases. Translation is required by these ORBs. factors localize A substrate...