A5003871970- Animal Virus Infections Studies
- Viral Infections and Vectors
- Virology and Viral Diseases
- Rabies epidemiology and control
- SARS-CoV-2 and COVID-19 Research
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Mosquito-borne diseases and control
- Leptospirosis research and findings
- Zoonotic diseases and public health
- Influenza Virus Research Studies
- Extracellular vesicles in disease
- Health, Environment, Cognitive Aging
- Viral Infections and Immunology Research
- Yersinia bacterium, plague, ectoparasites research
- Respiratory viral infections research
- HIV Research and Treatment
- RNA regulation and disease
- Bacterial Infections and Vaccines
- Streptococcal Infections and Treatments
- RNA Research and Splicing
- Plant Virus Research Studies
- Microbial infections and disease research
The University of Sydney
2024
Taronga Conservation Society Australia
2024
Processus Infectieux en Milieu Insulaire Tropical
2015-2023
Inserm
2016-2023
Institut de Recherche pour le Développement
2016-2023
Centre National de la Recherche Scientifique
2016-2023
Aix-Marseille Université
2021-2022
Centre de Recherche et de Veille sur les Maladies Emergentes dans l’Océan Indien
2014-2021
Méditerranée Infection Foundation
2020
Peuplements végétaux et bioagresseurs en milieu tropical
2017
Extracellular vesicles (EVs), produced during viral infections, are of emerging interest in understanding infectious processes and host–pathogen interactions. EVs exosomes particular have the natural ability to transport nucleic acids, proteins, other components cellular or origin. Thus, they participate intercellular communication, immune responses, pathophysiological processes. Some viruses known hijack cell production content for their benefit. Here, we investigate whether two pathogenic...
It is commonly held that there a fundamental relationship between genome size and error rate, manifest as notional “error threshold” sets an upper limit on sizes. The sizes of RNA viruses, which have intrinsically high mutation rates due to lack mechanisms for correction, must therefore be small avoid accumulating excessive number deleterious mutations will ultimately lead population extinction. proposed exceptions this evolutionary rule are viruses from the order Nidovirales (such...
Report3 March 2022Open Access Transparent process A simple reverse genetics method to generate recombinant coronaviruses Julien Mélade Corresponding Author [email protected] orcid.org/0000-0003-1050-6091 Unité des Virus Émergents (UVE: Aix-Marseille Univ-IRD 190-Inserm 1207), Marseille, France Contribution: Conceptualization, Resources, Data curation, Software, Formal analysis, Validation, Visualization, Methodology, Writing - original draft, review & editing Search for more papers by this...
The Paramyxoviridae form an increasingly diverse viral family, infecting a wide variety of different hosts. In recent years, they have been linked to disease emergence in many animal populations and humans. Bats rodents identified as major capable harboring paramyxoviruses, host shifting between these animals is likely be important driving factor the underlying evolutionary processes that eventually lead emergence. Here, we studied paramyxovirus circulation within endemic introduced wild...
Leptospirosis is a bacterial zoonosis caused by pathogenic Leptospira for which rats are considered as the main reservoir. Disease incidence higher in tropical countries, especially insular ecosystems. Our objectives were to determine current burden of leptospirosis Seychelles, country ranking first worldwide according historical data, establish epidemiological links between animal reservoirs and human disease, identify drivers transmission.A total 223 patients with acute febrile symptoms...
An eco-epidemiological investigation was carried out on Madagascar bat communities to better understand the evolutionary mechanisms and environmental factors that affect virus transmission among species in closely related members of genus Morbillivirus, currently referred as Unclassified Morbilli-related paramyxoviruses (UMRVs). A total 947 bats were investigated originating from 52 capture sites (22 caves, 18 buildings, 12 outdoor sites) distributed over different bioclimatic zones island....
We provide serological evidence of lyssavirus circulation among bats on southwestern Indian Ocean (SWIO) islands. A total 572 belonging to 22 species were collected Anjouan, Mayotte, La Réunion, Mauritius, Mahé and Madagascar screened by the Rapid Fluorescent Focus Inhibition Test for presence neutralising antibodies against two main rabies related lyssaviruses circulating African continent: Duvenhage (DUVV) Lagos bat (LBV), representing phylogroups I II, respectively. 97 42 sera able...
We report a fatal case of coxsackievirus B4 chronic infection in 30-year-old woman with diagnosis myelin oligodendrocyte glycoprotein antibody-associated disorder controlled by rituximab monotherapy for 3 years. Initially presenting as self-limited meningitis, the remained silent 8 months before sudden onset fulminant myocarditis. Analysis complete genome showed that same virus was responsible both episodes.
Leptospirosis, an environmental infectious disease of bacterial origin, is the with highest associated mortality in Seychelles. In small island territories, occurrence spatially heterogeneous and a better understanding factors that contribute to presence bacteria would help implement targeted control. The present study aimed at identifying main parameters correlated animal reservoirs distribution Leptospira infection order delineate habitats prevalence. We used previously published dataset...
Abstract One portion of the family Paramyxoviridae is a group Unclassified Morbilli - Related Viruses ( UMRV ) recently recognized in wild small mammals. At global level, evolutionary history these viruses not properly understood and relationships between their hosts still remain largely unstudied. The present study revealed, for first time, that Rodentia associated emerged from common ancestor southern Africa more than 4000 years ago. Sequenced originating different regions world, clustered...
Jingchuvirales (negative-sense RNA viruses) were initially discovered in invertebrates, with both exogenous and endogenous jingchuviruses subsequently identified fish, reptiles mammals. To date, have only been described metagenomically. By screening primary tumor tissues cell lines from the endangered Tasmanian devil (Sarcophilus harrisii), we isolated chu-like virus (TDCV) cultures of facial disease (DFTD) cells. Cell infection experiments demonstrated active replication cells, but not...
Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most challenging infectious disease pig populations causing devastating economic loss to swine industry. Reverse genetics allow engineer modified viruses such attenuated strains for vaccine development. Some reverse genetic systems were described PRRSVs but, due genome complexity PRRSVs, construction modification remain laborious time-consuming. In this study, we a approach based on "Infectious-Subgenomic Amplicons"...
Extracellular vesicles (EVs), produced during viral infections, are of emerging interest in understanding infectious processes and host-pathogen interactions. EVs exosomes particular have the natural ability to transport nucleic acids, proteins, other components cellular or origin. Thus, they participate intercellular communication, immune responses, pathophysiological processes. Some viruses known hijack cell production content for their benefit. Here, we investigated whether two pathogenic...
Abstract There is a need for simple reverse genetics methods to decipher the biological properties of animal and human coronaviruses. Here, we attempted rescue recently emerged severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Feline enteric (FeCoV) using rapid “Infectious-Subgenomic Amplicons” (ISA) method. For each virus, transfection into permissive cells eight overlapping subgenomic cDNA fragments covering entire genome allowed reconstruction complete virus generated...
Abstract Engineering recombinant viruses is capital for deciphering the biology of emerging viral pathogens such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, large size coronaviruses genome makes reverse genetics methods challenging.Here we describe a simple method based on “infectious subgenomic amplicons” (ISA) technology to generate infectious with no need reconstructing full genomic cDNA. The was applied SARS-CoV-2 and feline enteric coronavirus, allowed...
Abstract There is a need for simple reverse genetics methods to decipher the biological properties of animal and human coronaviruses. Here, we attempted rescue recently emerged severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Feline enteric (FeCoV) using rapid “Infectious-Subgenomic Amplicons” (ISA) method. For each virus, transfection into permissive cells eight overlapping subgenomic cDNA fragments covering entire genome allowed reconstruction complete virus generated...