A5067959459- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Monoclonal and Polyclonal Antibodies Research
- Protein purification and stability
- Crystallography and molecular interactions
- Viral Infectious Diseases and Gene Expression in Insects
- Silk-based biomaterials and applications
- Biochemical and Structural Characterization
- Biosimilars and Bioanalytical Methods
- Neurobiology and Insect Physiology Research
- Phytochemical compounds biological activities
- Glycosylation and Glycoproteins Research
- Heme Oxygenase-1 and Carbon Monoxide
- RNA and protein synthesis mechanisms
- Hemoglobin structure and function
- Nanofabrication and Lithography Techniques
- Computational Drug Discovery Methods
- Invertebrate Immune Response Mechanisms
- Erythrocyte Function and Pathophysiology
Boehringer Ingelheim (Germany)
2019-2024
Biocat
2021
University of Bayreuth
2016-2018
University of Duisburg-Essen
2010
Abstract Dragline silk is the most prominent amongst spider silks and comprises two types of major ampullate spidroins (MaSp) differing in their proline content. In natural spinning process, conversion soluble MaSp into a tough fiber is, other factors, triggered by dimerization conformational switching helical amino-terminal domains (NRN). Both processes are induced protonation acidic residues upon acidification along duct. Here, structure monomer-dimer-equilibrium domain NRN1 Latrodectus...
Major ampullate spidroin (MaSp) assembly starts in the abdomen of spider, where spidroins are stored as a liquid dope at high concentration. The is squeezed into spinning duct, and finished upon drawing fibers. Unwanted aggregation solution gland suppressed by prestructuring micelle-like assemblies, with their hydrophobic stretches being hidden from solvent hydrophilic nonrepetitive amino (NRN) carboxy (NRC) terminal domains exposed on micelle surface. Conversion fluid solid fiber induced...
Spider silk proteins comprise a repetitive core domain with polyalanine and glycine/proline-rich stretches flanked by highly conserved nonrepetitive N- C-terminal domains. The termini are responsive to assembly triggers, sensing changes in the ionic (H+, phosphate) mechanical (shear stress) environment along spinning duct. N-terminal dimerizes pH-dependent manner induced protonation of acidic residues. To date, dimerization spider domains has been individually investigated absence large In...
A vital part of biopharmaceutical research is decision making around which lead candidate should be progressed in early-phase development. When multiple antibody candidates show similar biological activity, developability aspects are taken into account to ease the challenges manufacturing potential drug candidate. While current strategies for assessment mainly focus on product stability, only limited information available how with minimal differences their primary structure behave during...
Asparagine (Asn) deamidation and aspartic acid (Asp) isomerization are common degradation pathways that affect the stability of therapeutic antibodies. These modifications can pose a significant challenge in development biopharmaceuticals. As such, early engineering selection chemically stable monoclonal antibodies (mAbs) substantially mitigate risk subsequent failure. In this study, we introduce novel silico approach for predicting sites by analyzing structural environment surrounding...
The physico-chemical suitability of perfluorocarbon-filled capsules as artificial oxygen carriers for blood substitutes is assessed on the example biodegradable poly(lactide-co-gylcolide) micro- and nanocapsules with a liquid content perfluorodecalin. morphology studied by confocal laser scanning microscopy using Nile red fluorescent marker. mechanical stability wall flexibility are examined atomic force microscopy. permeability capsule walls in connection uptake detected nuclear magnetic...
Biomimetic spidroin self-assembly allows formation of substrate-independent one-pot nanothin silk coatings.
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Abstract Monoclonal antibodies bind with high specificity to a wide range of diverse antigens, primarily mediated by their hypervariable complementarity determining regions (CDRs). The defined antigen binding loops are supported the structurally conserved β-sandwich framework light chain (LC) and heavy (HC) variable regions. LC genes encoded two separate loci, subdividing entity into kappa (LCκ) lambda (LCλ) isotypes that exhibit distinct sequence conformational preferences. In this work,...
Adequate stability, manufacturability, and safety are crucial to bringing an antibody-based biotherapeutic the market. Following concept of holistic in silico developability, we introduce a physicochemical description 91 market-stage biotherapeutics based on orthogonal molecular properties variable regions (Fvs) embedded different simulation environments, mimicking conditions experienced by antibodies during manufacturing, formulation, vivo. In this work, evaluation includes conformational...