A5067392105- Sperm and Testicular Function
- Bacterial Identification and Susceptibility Testing
- Reproductive Biology and Fertility
- Biosensors and Analytical Detection
- Epigenetics and DNA Methylation
- Genomics and Phylogenetic Studies
- Antibiotic Resistance in Bacteria
- Fish Ecology and Management Studies
- Reproductive Physiology in Livestock
- Toxic Organic Pollutants Impact
- Reproductive biology and impacts on aquatic species
- Gold and Silver Nanoparticles Synthesis and Applications
- Effects and risks of endocrine disrupting chemicals
- Spectroscopy Techniques in Biomedical and Chemical Research
- Pharmaceutical and Antibiotic Environmental Impacts
- Helminth infection and control
- Milk Quality and Mastitis in Dairy Cows
- Apelin-related biomedical research
- Cell Image Analysis Techniques
- Dermatological diseases and infestations
- Testicular diseases and treatments
- Streptococcal Infections and Treatments
- Identification and Quantification in Food
- Antimicrobial Resistance in Staphylococcus
- Per- and polyfluoroalkyl substances research
University of Inland Norway
2019-2023
Norwegian University of Life Sciences
2017-2021
Antimicrobial resistance (AMR) is a major health threat worldwide and the culture-based bacterial detection methods are slow. Surface-enhanced Raman spectroscopy (SERS) can be used to identify target analytes in real time with sensitivity down single-molecule level, providing promising solution for culture-free detection. We report fabrication of SERS substrates having tightly packed silver (Ag) nanoparticles loaded onto long silicon nanowires (Si NWs) grown by metal-assisted chemical...
Sperm DNA integrity is considered essential for successful transmission of the paternal genome, fertilization and normal embryo development. fragmentation index (DFI, %) has become a key parameter in swine artificial insemination industry to assess sperm integrity. Recently, some elite Norwegian Landrace boars (boars with excellent field fertility records), higher level DFI been observed. In order obtain better understanding this, study complexity integrity, liquid preserved semen samples...
Emerging new sequencing technologies have provided researchers with a unique opportunity to study factors related microbial pathogenicity, such as antimicrobial resistance (AMR) genes and virulence factors. However, the use of whole-genome sequence (WGS) data requires good knowledge bioinformatics involved, well necessary techniques. In this study, total nine Escherichia coli Klebsiella pneumoniae isolates from Norwegian clinical samples were sequenced using both MinION Illumina platforms....
Rapid and accurate diagnosis of causative pathogens in mastitis would minimize the imprudent use antibiotics and, therefore, reduce spread antimicrobial resistance. Whole genome sequencing offers a unique opportunity to study microbial community resistance (AMR) mastitis. However, complexity milk samples presence high amount host DNA from infected udders often make this very challenging.Here, we tested 24 bovine (18 six non-mastitis) using four different commercial kits (Qiagens' DNeasy®...
Persistent organic pollutants (POPs) are widespread in the environment and some may be neurotoxic. As we exposed to complex mixtures of POPs, aimed investigate how a POP mixture based on Scandinavian human blood data affects behaviour neurodevelopment during early life zebrafish. Embryos/larvae were series sub-lethal doses was examined at 96 h post fertilization (hpf). In order determine sensitivity window mixture, exposure models 6 48 hpf used. The expression genes related neurological...
Current state-of-the-art infection and antimicrobial resistance (AMR) diagnostics are based on culture-based methods with a detection time of 48-96 h. Therefore, it is essential to develop novel that can do real-time diagnoses. Here, we demonstrate the complimentary use label-free optical assay whole-genome sequencing (WGS) enable rapid diagnosis AMR. Our microscopy exploiting label-free, highly sensitive quantitative phase (QPM) followed by deep convolutional neural networks-based...
Norway is known for being one of the countries with lowest levels antimicrobial resistance (AMR). AMR, through acquired genes located on transposons or conjugative plasmids, horizontal transmission required a given bacteria to withstand antibiotics. In this work, bioinformatic analysis whole-genome sequences and hybrid assembled data from Escherichia coli, Klebsiella pneumoniae isolates Norwegian patients was performed. For detection putative plasmids in isolates, plasmid assembly mode...
In the dairy breeding industry, prediction of bull fertility in artificial insemination (AI) is important for efficient and economically sustainable production. However, it challenging to identify bulls with superior applying conventional vitro sperm assays. present study, functionality was investigated a multivariate model that could predict fertility. Two groups young Norwegian Red were selected, one inferior (18 bulls) (19 based on non-return rate after 56 days (NR56). Frozen-thawed semen...
Abstract In this study, the complexity of chromatin integrity was investigated in frozen‐thawed semen samples from 37 sires with contrasting fertility, expressed as 56‐day non‐return rates (NR56). Protamine deficiency, thiols, and disulfide bonds were assessed compared previously published data for DNA fragmentation index (DFI) high stainability (HDS). addition, vitro embryo development sperm methylation using 16 these bulls. The percentages DFI HDS negatively associated NR56 cleavage rate...
Male and female mice pups were exposed to a low high dose of human relevant mixture persistent organic pollutants (POPs) during pregnancy lactation. Most compounds detected in the dams found offspring brains. The exhibited changed expression hippocampal genes involved cognitive function (Adora2a, Auts2, Crlf1, Chrnb2, Gdnf, Gnal, Kcnh3), neuroinflammation (Cd47, Il1a), circadian rhythm (Per1, Clock), redox signalling (Hmox2) aryl hydrocarbon receptor activation (Cyp1b1). A few differentially...
Testicular torsion/detorsion is one of the important emergencies that requires fast surgical intervention. This study aimed to investigate effects Salvia miltiorrhiza hydroalcoholic extract combined with verapamil on testicular ischaemia/reperfusion damage in Wistar albino rats. All animals were distributed 3 groups (n = 8), including sham-operated group, (TD) group and + pretreatment 200 mg/kg 0.3 (SMV) group. Oxidative stress biomarkers (MDA, GPx, CAT TAC) both plasma tissue, sperm...
Aim: To our knowledge, the relationship between simple renal cysts, hypertension and three significant genes of renin-angiotensin system ( AGT, AT1R ACE1) has not been studied. The present study was designed to search for possible relationships these polymorphic components, cysts in Shiraz province (Iran). Methods: A total 160 participants were recruited from Motahari Clinic at University Medical Sciences. subjects divided into four main groups. Detection ACE1 genotype performed with a...
Genomic selection in modern farming demands sufficient semen production young bulls. Factors affecting quality and capacity bulls are not well understood; DNA methylation, a complicated phenomenon sperm cells, is one such factors. In this study, fresh frozen-thawed samples from the same Norwegian Red (NR) at both 14 17 months of age were examined for chromatin integrity parameters, ATP content, viability, motility. Furthermore, reduced representation bisulfite libraries constructed according...
Abstract The current state-of-the-art infection and antimicrobial resistance diagnostics (AMR) is based mainly on culture-based methods with a detection time of 48-96 hours. Slow diagnoses lead to adverse patient outcomes that directly correlate the taken administer optimal antimicrobials. Mortality risk doubles 24-hour delay in providing appropriate antibiotics cases bacteremia. Therefore, it essential develop novel can promptly accurately diagnose microbial infections at both species...
ABSTRACT The world health organization considers antimicrobial resistance (AMR) to be a critical global public problem. Conventional culture-based methods that are used detect and identify bacterial infection slow. Thus, there is growing need for the development of robust, cost-effective, fast diagnostic solutions identification pathogens. Surface-enhanced Raman spectroscopy (SERS) can target analytes with sensitivity down single-molecule level. Here, we developed SERS chip by optimizing...
We have investigated the enhanced Raman spectra of AMR bacteria strains E. coli using silver coated silicon nanowires SERS assay. Three different strains, <i>E. CCUG17620, NCTC 13441</i>, and <i>A239</i>, were detected two excitation laser wavelengths. found stable spectrum 785 nm as opposed to 532 nm. Future development SERS-chip could offer a reliable platform for direct identification pathogen in bio-fluid samples at level.
Cold storage and freezing/thawing of milt may affect sperm functionality the subsequent fertilization ability milt. This study aimed to investigate quality parameters potential Atlantic salmon milt, stored cold subsequently cryopreserved, using different conditions. The objective was also assess if analysis metabolites DNA methylation signatures could be applicable further elucidate following preservation. Milt samples were collected from eight mature males for 4 days at 2°C 8°C. Samples...