A5082908711- Monoclonal and Polyclonal Antibodies Research
- Advanced Biosensing Techniques and Applications
- Advanced biosensing and bioanalysis techniques
- Glycosylation and Glycoproteins Research
- Ovarian cancer diagnosis and treatment
- Molecular Junctions and Nanostructures
- Analytical Chemistry and Chromatography
- Chromatography in Natural Products
- Microfluidic and Capillary Electrophoresis Applications
University of Kansas
2023-2025
Rowan University
2024
University of Notre Dame
2023
Background: The ovarian cancer biomarker CA125 is a peptide epitope found in multiple tandem repeat domains of the mucin MUC16. Although efforts have been undertaken to characterize interaction between and its clinically used antibodies, molecular nature epitope(s) remains undefined. A recent revision model MUC16 provides an opportunity fully binding CA125-specific antibodies region Objectives: objective this study was expressed proteins from as part longer-term effort identify epitopes with...
The high affinity of the biotin–streptavidin interaction has made this non-covalent coupling an indispensable strategy for immobilization and enrichment biomolecular reagents. However, irreversible nature bond renders surfaces functionalized using permanently modified not amenable to regeneration strategies that could increase assay reusability throughput. To utility biotinylated targets, we here introduce a method reversibly immobilizing thrombin-binding aptamers onto Ni-nitrilotriacetic...
BACKGROUND: Despite its importance in the clinical management of ovarian cancer, CA125 biomarker – located on mucin protein MUC16 is still not completely understood. Questions remain about MUC16’s function and structure, specifically identity location epitopes. OBJECTIVE: The goal this study was to characterize interaction individual recombinant repeats from tandem repeat domain with antibodies used II test. METHODS: Using E. coli expression, we isolated nine putative antigenic CA125. Amino...
Surface plasmon resonance (SPR) is a popular real-time technique for the measurement of binding affinity and kinetics, bench-top instruments combine affordability ease use with other benefits technique. Biomolecular ligands labeled 6xHis tag can be immobilized onto sensing surfaces presenting Ni2+-nitrilotriacetic acid (NTA) functional group. While Ni-NTA immobilization offers many advantages, including ability to regenerate reuse sensors, its lead signal variability between experimental...
HPLC continues to be one of the most widely used measurement techniques for chemical analysis. Capillary LC, which utilizes narrow diameter columns operated at lower flow rates than analytical-scale gain adoption based on its reduced mobile phase consumption and increased sensitivity when coupled MS detection. This tutorial offers practical insights into critical aspects translating separations capillary scale. The selection pumping systems, detectors, potential performance loss due...
Despite its importance in the clinical management of ovarian cancer, CA125 biomarker-located on mucin protein MUC16-is still not completely understood. Questions remain about MUC16's function and structure, specifically identity location epitopes.The goal this study was to characterize interaction individual recombinant repeats from tandem repeat domain MUC16 with antibodies used II test.Using E. coli expression, we isolated nine putative antigenic CA125. Amino acid composition confirmed by...