A5002601950- Steroid Chemistry and Biochemistry
- Phytochemistry and Bioactive Compounds
- Phytochemical Studies and Bioactivities
- Chromatography in Natural Products
- Natural product bioactivities and synthesis
- Cancer therapeutics and mechanisms
- Marine Sponges and Natural Products
- Enzyme-mediated dye degradation
- Pharmaceutical and Antibiotic Environmental Impacts
- Analytical chemistry methods development
- Bioactive Compounds and Antitumor Agents
- Microbial Natural Products and Biosynthesis
Anhui University
2024
Jinan University
2018
A permissive steroid glycosyltransferase (UGT74AN1) from Asclepias curassavica exhibited robust capabilities for the regiospecific C3 glycosylation of cardiotonic steroids and C21 precursors, unprecedented promiscuity toward 53 structurally diverse natural unnatural compounds to form O-, N-, S-glycosides, along with catalytic reversibility a one-pot transglycosylation reaction. These findings highlight UGT74AN1 as first catalyst exhibit significant potential bioactive molecules in drug discovery.
This study presents an efficient strategy based on liquid–liquid extraction with three-phase solvent system and high speed counter-current chromatography for rapid enrichment separation of epimers minor bufadienolide from toad meat. The reflux conditions were optimized by response surface methodology first, a novel composed n-hexane/methyl acetate/acetonitrile/water (3:6:5:5, v/v) was developed the crude extract. integrative process could enrich complex matrix efficiently minimize loss...
The glycosyltransferase OleD variant as a catalyst for the glycosylation of four pairs epimers cardiotonic steroids (CTS) are assessed. results this study demonstrated that OleD-catalyze CTS is significantly influenced by configuration at C-3 and A/B fusion mode. 3β-OH ring cis favoured (ASP). An epoxide C-14 C-15 further increases bioconversion rate; while an acetyl group C-16 lactone type C-17 did not influence biotransformation. A high conversion rate corresponded to low K m value....