A5102994599- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Bio-sensing Technologies
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Cellular Mechanics and Interactions
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- Cell Image Analysis Techniques
- Cancer, Stress, Anesthesia, and Immune Response
- Polymer Surface Interaction Studies
- Nanoparticle-Based Drug Delivery
- Estrogen and related hormone effects
- Immune cells in cancer
- Nanoplatforms for cancer theranostics
University of Gothenburg
2022
Wisconsin Institutes for Discovery
2019-2020
University of Wisconsin–Madison
2016-2020
University of Wisconsin Carbone Cancer Center
2020
Madison Group (United States)
2020
The University of Texas Health Science Center at Houston
2018
Abstract The estrogen receptor (ER) regulates the survival and growth of breast cancer cells, but it is less clear how components tissue microenvironment affect ER-mediated responses. We set out to test human mammary fibroblasts (HMFs) modulate ER signaling downstream cellular exposed an organotypic model consisting a collagen-embedded duct structure lined with MCF7 cells 17-β estradiol (E2), without HMFs in surrounding matrix. grown as ductal structures were polarized proliferated at rates...
The tumour microenvironment (TME) has recently drawn much attention due to its profound impact on development, drug resistance and patient outcome. There is an increasing interest in new therapies that target the TME. Nonetheless, most established vitro models fail include essential cues of Microfluidics can be used reproduce TME hence provide valuable insight evolution sensitivity. However, microfluidics remains far from well-established mainstream molecular cell biology methods. Therefore,...
Extracellular matrix (ECM)-mimicking hydrogel scaffolds have greatly improved the physiological relevance of in vitro assays but introduce another dimension that creates variability cell-related readouts when compared to traditional two-dimensional cells-on-plastic assays. We developed a synthetic poly(ethylene glycol) (PEG)-based ECM-mimicking and tested it against two gold standard animal-based naturally derived MCF7 cell response. used percent coefficient variation (CV) as metric evaluate...
Abstract The prostate tumor microenvironment (TME) is strongly immunosuppressive; it largely driven by alteration in cell phenotypes (i.e. tumor-associated macrophages and exhausted cytotoxic T cells) that result pro-tumorigenic conditions growth. A greater understanding into how these altered immune are developed could potentially be reversed would provide important insights improved treatment efficacy for cancer. Here, we report a microfluidic model of the TME mimics ducts across various...
Luminal geometries are common structures in biology, which challenging to mimic using conventional vitro techniques based on the use of Petri dishes. In this context, microfluidic systems can lumen geometry, enabling a large variety studies. However, most models still rely polydimethylsiloxane (PDMS), material that is not amenable for high-throughput fabrication and presents some limitations compared with other materials such as polystyrene. Thus, we have developed device array generate...
B cells interact with T follicular helper (Tfh) in germinal centers (GCs) to generate high-affinity antibodies. Much less is known about how cognate T-B-cell interactions influence Th that enter circulation and peripheral tissues. Therefore, we generated mice lacking MHC-II expressing and, by thoracic duct cannulation, analyzed the efferent lymph at defined intervals post-immunization. Focusing on gut-draining mesenteric nodes (MLNs), show antigen-specific α4β7 + gut-homing effector prior...