A5019845242- Phytochemicals and Antioxidant Activities
- Receptor Mechanisms and Signaling
- Phytochemistry and Biological Activities
- Drug Transport and Resistance Mechanisms
- Essential Oils and Antimicrobial Activity
- Cancer-related gene regulation
- Wnt/β-catenin signaling in development and cancer
- Neuropeptides and Animal Physiology
- Epigenetics and DNA Methylation
- Pancreatic function and diabetes
- RNA modifications and cancer
- Traditional and Medicinal Uses of Annonaceae
- Diabetes and associated disorders
- Vitamin D Research Studies
- Inflammatory mediators and NSAID effects
- Natural Compound Pharmacology Studies
- Chemical synthesis and alkaloids
- MicroRNA in disease regulation
- RNA Research and Splicing
- Vitamin K Research Studies
- Natural product bioactivities and synthesis
- Thyroid Disorders and Treatments
- Flowering Plant Growth and Cultivation
- Vitamin C and Antioxidants Research
- Alkaloids: synthesis and pharmacology
Karolinska Institutet
2022-2023
Medical University of Warsaw
2016-2023
Centrum Kopernika Badań Interdyscyplinarnych
2019-2020
The polyphenol content and antioxidant capacity of hyperforin hypericin-standardized H. perforatum L. extracts may vary due to the harvest time. In this work, ethanol ethanol-water air-dried lyophilized flowers L., collected throughout a vegetation season in central Poland, were studied. Air-dried had higher (371 mg GAE/g) flavonoid (160 CAE/g) content, DPPH radical scavenging (1672 DPPH/g), ORAC (5214 µmol TE/g) FRAP (2.54 mmol Fe2+/g) than (238 GAE/g, 107 CAE/g, 1287 DPPH/g, 3313 TE/g 0.31...
Geissospermum species are medically important plants due to their health-promoting effects. The objective of this study was determine the antioxidant ability and antiproliferative cytotoxic effects infusions, tinctures, ethanolic extracts reticulatum barks in relation contents total phenolics flavonoids. Seven samples were collected various regions Peruvian Amazonia. We found that amount studied products varied from 212.40 ± 0.69 1253.92 11.20 mg GAE/kg. In our there is a correlation (R2 =...
Background and Purpose Wnt binding to Frizzleds (FZD) is a crucial step that leads the initiation of signalling cascades governing multiple processes during embryonic development, stem cell regulation adult tissue homeostasis. Recent efforts have enabled us shed light on Wnt–FZD pharmacology using overexpressed HEK293 cells. However, assessing ligand at endogenous receptor expression levels important due differential behaviour in native environment. Here, we study FZD paralogue, 7 , analyse...
GPR61 is an orphan receptor that belongs to Class A of G-protein-coupled receptors. It has been reported a constitutive activity and couples Gα<sub>s</sub>. In the present study, we characterized function ligand binding by experimental molecular docking studies. We demonstrated heterologous expression in HEK293 cells enhanced cAMP synthesis response forskolin, whereas basal was unaffected. 5-(Nonyloxy)tryptamine inhibited forskolin-stimulated production GPR61-expressing cells....
RNA regulation can be performed by a second targeting molecule, such as in the microRNA mechanism. Selective 2′-hydroxyl acylation analyzed primer extension (SHAPE) probes structure of molecules and resolve RNA:protein interactions, but RNA:RNA interactions have not yet been addressed with this technique. Here, we apply SHAPE to investigate RNA-mediated binding processes RNA:RNA-RBP complexes. We use R NA:RN A b inding S HAPE (RABS) microRNA-34a ( miR-34a ) its mRNA target, silent...
Geissospermum species are widely used in folk medicine the Amazon region.This study was conducted to determine total phenolic and flavonoid contents of three tinctures reticulatum barks from Peruvian correlate these antioxidant activities stability.Total content compounds (from 694.91 1430.67 mg GAE/kg) flavonoids (575.23-815.65 CAE/kg) were found by spectrophotometric methods.The obtained values interpreted artificial neural networks describe most beneficial conditions for tinctures.All...
Biologicznie czynna forma witaminy D, czyli 1,25-dwuhydroksywitamina D3, przez lata była uważana jedynie za czynnik zapewniający odpowiedni rozwój układu kostnego i stosowana w profilaktyce zachorowań na krzywicę. Dziś coraz więcej badań wskazuje, że jest to związek mający wpływ ponad 200 genów większość narządów ludzkiego organizmu. Termin witamina D odnosi się zarówno do (substancja egzogenna dostarczana z zewnątrz wraz pożywieniem), jak również prohormonu (związek endogenny produkowany...
A new alkaloid, geissospermiculatine was characterized in Geissospermum reticulatum A. H. Gentry bark (Apocynaceae). Here, following a simplified isolation protocol, the structure of alkaloid elucidated through GC-MS, LC-MS/MS, 1D, and 2D NMR (COSY, ROESY, HSQC, HMBC, 1H-15N HMBC). Cytotoxic properties were evaluated vitro on malignant THP-1 cells, results demonstrated that cytotoxicity (30 μg/mL) comparable with staurosporine (10 μM). Additionally, toxicity tested zebrafish (Danio rerio)...
Tom 75 • nr 8 2019 klasę receptorów, F, stanowią Frizzleds [8].Receptory te są głównie kojarzone ze ścieżką sygnałową WNT/β-katenina i często klasyfikowane wręcz jako tylko "podobne do GPCR" (ang.GPCR-like) [9].Jednak w ostatnim czasie opublikowanych zostało kilka ważnych badań
Review of the drugs currently used in treatment pain osteoarthritisPain associated with musculoskeletal system disorders is very often reason why patients seek medical advice.This also case for osteoarthritis (OA) where first and dominant symptom which greatly reduces quality life.OA most common disorder system.In fact, this disease affects approximately 17% Polish society, therefore early diagnosis correct are utmost significance.Along these lines, it well-known that untreated can lead to...
ABSTRACT WNT binding to Frizzleds (FZD) is a crucial step that leads the initiation of signalling cascades governing multiple processes during embryonic development, stem cell regulation and adult tissue homeostasis. Recent efforts have enabled us shed light on WNT-FZD pharmacology in overexpressed HEK293 systems. However, it important assess ligand at endogenous receptor levels as there might be differential behaviour native environment. Here, we focus one FZD paralogue: 7 , study its...