A5048646352- HIV Research and Treatment
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- T-cell and Retrovirus Studies
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Immune Cell Function and Interaction
- CRISPR and Genetic Engineering
- CAR-T cell therapy research
- HIV/AIDS drug development and treatment
- HIV/AIDS Research and Interventions
- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Immunotherapy and Immune Responses
- Advanced biosensing and bioanalysis techniques
- T-cell and B-cell Immunology
- Hematopoietic Stem Cell Transplantation
- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Single-cell and spatial transcriptomics
- HIV-related health complications and treatments
- Nanoparticle-Based Drug Delivery
- interferon and immune responses
- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
University of California, Los Angeles
2016-2025
Black AIDS Institute
2014-2024
Broad Center
2015-2022
UCLA Jonsson Comprehensive Cancer Center
1990-2014
UCLA Health
2007-2013
California NanoSystems Institute
2012
Portola Pharmaceuticals (United States)
2011
Laboratory of Molecular Genetics
2010
Pediatrics and Genetics
2007
Bipar
2007
Genome-wide genetic approaches have proven useful for examining pathways of biological significance in model organisms such as Saccharomyces cerevisiae, Drosophila melanogastor, and Caenorhabditis elegans, but similar techniques difficult to apply mammalian systems. Although manipulation the murine genome has led identification genes their function, this approach is laborious, expensive, often leads lethal phenotypes. RNA interference (RNAi) an evolutionarily conserved process gene silencing...
Our laboratory has recently characterized a population of cells from adipose tissue, termed processed lipoaspirate (PLA) cells, which have multi-lineage potential similar to bone-marrow-derived mesenchymal stem (MSCs). This study is the first comparison PLA and MSCs isolated same patient. No significant differences were observed for yield adherent stromal growth kinetics, cell senescence, differentiation capacity, gene transduction efficiency. Adipose tissue an abundant easily procured...
Human immunodeficiency virus (HIV) is the causative agent of acquired immune deficiency syndrome (AIDS). A large number AIDS patients show evidence neurologic involvement, known as AIDS-related subacute encephalopathy, which has been correlated with presence HIV in brain. In this study, two genetically distinct but related viruses were isolated from one patient different sources central nervous system: brain tissue and cerebrospinal fluid. Both found to replicate peripheral blood...
Double-stranded RNAs ≈21 nucleotides long [small interfering RNA (siRNA)] are recognized as powerful reagents to reduce the expression of specific genes. To use them protect cells against viral infection, effective methods for introducing siRNAs into primary required. Here, we describe success in constructing a lentivirus-based vector introduce HIV-1 coreceptor, CCR5, human peripheral blood T lymphocytes. With high-titer stocks, >40% lymphocytes could be transduced, and potent CCR5-siRNA...
Some strains of human immunodeficiency virus type 1 (HIV-1) can infect primary monocytes and monocyte-derived macrophages in vitro. In this report, the effect cytokines on production one these that shows a tropism for mononuclear phagocytes, designated HIV-1 JR-FL , was studied. Primary peripheral blood phagocytes infected with were treated hematopoietic factors: granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage (GM-CSF), interleukin-3 (IL-3), macrophage (M-CSF),...
Confirmed infection with HTLV-II (human T cell leukemia virus type II) has been described only in rare cases. The major limitation to serological diagnosis of the difficulty distinguishing from HTLV-I I) infection, because substantial cross-reactivity between viruses. A sensitive modification polymerase chain reaction method was used provide unambiguous molecular evidence that a significant proportion intravenous drug abusers are infected HTLV, and majority these individuals rather than...
Cancer immunotherapy has reshaped the landscape of cancer treatment. However, its efficacy is still limited by tumor immunosuppression associated with excessive production lactate cells. Although extensive efforts have been made to reduce concentrations through inhibition dehydrogenase, such inhibitors disrupt metabolism healthy cells, causing severe nonspecific toxicity. We report herein a nanocapsule enzyme therapeutic based on oxidase, which reduces and releases immunostimulatory hydrogen...
THE human T-cell lymphotropic viruses Type I (HTLV-I) and II (HTLV-II) the bovine leukemia virus, which are members of a family leukemogenic mammalian retroviruses, share some same structural functional characteristics. HTLV-I has been implicated as causative agent adult leukemia, lymphoid malignant disease endemic to southern Japan Caribbean area.1 2 3 4 5 6 7 8 Bovine virus recognized cause specific diseases cattle.9 10 11 The role HTLV-II in cancer is unclear. This retrovirus with nucleic...
The human T-cell leukemia viruses HTLV-I and HTLV-II are unique among the transforming retroviruses of vertebrates in their ability to transform T cells vitro close association with malignancies (T-cell lymphomas leukemia). Their genomes relatively simple, containing genes gag , pol env a 3′ region termed "X." This may be responsible for potential viruses. existence proteins encoded by has been postulated on basis multiple open reading frames. In present study this is shown contain gene...
Expression of human immunodeficiency virus-type 1 (HIV-1) Vpr after productive infection T cells induces cell cycle arrest in the G2 phase cycle. In absence de novo expression, HIV-1 packaged into virions still induced arrest. Naturally noninfectious virus or rendered defective for by reverse transcriptase protease inhibitors were capable inducing Vpr-mediated These results suggest a model whereby both infectious and vivo, such as those surrounding follicular dendritic cells, participate...
We describe a child who was identified shortly after birth as infected with the human immunodeficiency virus type 1 (HIV-1), but whose infection appears to have completely cleared. Asymptomatic HIV-1 diagnosed in mother during fourth month of pregnancy. The infant delivered vaginally at 36 weeks, received no blood products, and not breast-fed.HIV-1 detected by culture infant's peripheral-blood mononuclear cells 19 51 days age. Plasma from also culture-positive for age DNA polymerase chain...
The human T-cell leukemia viruses (HTLV) are associated with malignancies in man and will transform normal T cells vitro. mechanism of malignant transformation by HTLV is unknown but appears to be distinct from that other classes retroviruses, which induce through viral or cellular oncogenes. Recently a new gene, termed x , was identified HTLV. This gene has been hypothesized the transforming because its conservation within class retroviruses. By vitro mutagenesis HTLV-II it now demonstrated...
ABSTRACT We recently demonstrated that a soluble protein, Gas6, can facilitate viral entry by bridging envelope phosphatidylserine to Axl, receptor tyrosine kinase expressed on target cells. The interaction between phosphatidylserine, and Axl was originally shown be molecular mechanism through which phagocytes recognize exposed dead Since our initial report, several groups have confirmed Axl/Gas6, as well other receptors, of dengue, West Nile, Ebola viruses. Virus binding is now generalized...
RNAi is a powerful method for suppressing gene expression that has tremendous potential therapeutic applications. However, because endogenous plays role in normal cellular functions, delivery and of siRNAs must be balanced with safety. Here we report successful stable primates directed to chemokine (c-c motif) receptor 5 (CCR5) introduced through CD34+ hematopoietic stem/progenitor cell transplant. After reconstitution, date 14 months after transplant, observe stably marked lymphocytes...