A5074983993- Advanced Fluorescence Microscopy Techniques
- Force Microscopy Techniques and Applications
- Lipid Membrane Structure and Behavior
- Advanced biosensing and bioanalysis techniques
- Lysosomal Storage Disorders Research
- Advanced Biosensing Techniques and Applications
- Cell Adhesion Molecules Research
- Cell Image Analysis Techniques
- Nanopore and Nanochannel Transport Studies
- Advanced Electron Microscopy Techniques and Applications
- Calcium signaling and nucleotide metabolism
- Anodic Oxide Films and Nanostructures
- Cellular transport and secretion
- RNA Interference and Gene Delivery
Yale University
2023-2024
Research Network (United States)
2024
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2022
National Institutes of Health
2022
Lysosomes receive extracellular and intracellular cholesterol redistribute it throughout the cell. Cholesterol egress from lysosomes is critical for homeostasis, its failure underlies pathogenesis of genetic disorders such as Niemann-Pick C (NPC) disease. Here we report that BLOC one-related complex (BORC)-ARL8-homotypic fusion protein sorting (HOPS) ensemble required free storage esterified in lipid droplets. Depletion BORC, ARL8, or HOPS does not alter localization lysosomal transmembrane...
The oligomeric organization of membrane proteins in native cell membranes is a critical regulator their function. High-resolution quantitative measurements assemblies and how they change under different conditions are indispensable to the understanding protein biology. We report single-molecule imaging technique (Native-nanoBleach) determine distribution directly from at an effective spatial resolution ∼10 nm. achieved this by capturing target "native nanodiscs" with proximal environment...
Abstract The native membrane environment profoundly influences every aspect of protein (MP) biology. Despite this, the most prevalent method studying MPs uses detergents to disrupt and remove this vital context, impeding our ability decipher local molecular context its effect. Here we develop a proteome-wide platform that enables rapid spatially resolved extraction target directly from cellular membranes into nanodiscs maintain using library membrane-active polymers. We accompany with an...
Abstract The intricate molecular environment of the native membrane profoundly influences every aspect protein (MP) biology. Despite this, most prevalent method studying MPs uses detergent-like molecules that disrupt and remove this vital local context. This severely impedes our ability to quantitatively decipher context comprehend its regulatory role in structure, function, biogenesis MPs. Using a library membrane-active polymers we have developed platform for high-throughput analysis...
This is a protocol to conduct Native Nanobleach of native nanodiscs.