A5043384994- Bacteriophages and microbial interactions
- Biochemical and Structural Characterization
- Bacterial Genetics and Biotechnology
- Cardiomyopathy and Myosin Studies
- Trypanosoma species research and implications
- Antimicrobial Resistance in Staphylococcus
- Research on Leishmaniasis Studies
- Cell Adhesion Molecules Research
- Monoclonal and Polyclonal Antibodies Research
- Cellular Mechanics and Interactions
- Mast cells and histamine
- Peptidase Inhibition and Analysis
- Biochemical and Molecular Research
- Acute Lymphoblastic Leukemia research
- Glycosylation and Glycoproteins Research
- T-cell and B-cell Immunology
- T-cell and Retrovirus Studies
- Vector-Borne Animal Diseases
- Neurotransmitter Receptor Influence on Behavior
- Microbial Natural Products and Biosynthesis
- Tryptophan and brain disorders
- Microbial Metabolism and Applications
- Animal Disease Management and Epidemiology
- Psychedelics and Drug Studies
- Natural product bioactivities and synthesis
University of Helsinki
2020-2024
University of Jyväskylä
2018-2024
Czech Academy of Sciences, Institute of Biotechnology
2021-2023
University of Molise
2017
Abstract Psychedelics produce fast and persistent antidepressant effects induce neuroplasticity resembling the of clinically approved antidepressants. We recently reported that pharmacologically diverse antidepressants, including fluoxetine ketamine, act by binding to TrkB, receptor for BDNF. Here we show lysergic acid diethylamide (LSD) psilocin directly bind TrkB with affinities 1,000-fold higher than those other psychedelics antidepressants distinct but partially overlapping sites within...
Epidemiological studies have shown that a reduced risk of chronic diseases such as cancer and cardiovascular is correlated with regular consumption fruits vegetable, many which are rich in polyphenols. The additive synergistic effect phytochemicals vegetables may reduce related to oxidative stress human body. Olea europaea L. leaf phenolic components, been proposed play role prevention. purpose this study was identify the main components (cv. Leccino) preserved during decoction preparation,...
Abstract Actin polymerization generates forces for cellular processes throughout the eukaryotic kingdom, but our understanding of ‘ancient’ actin turnover machineries is limited. We show that, despite > 1 billion years evolution, pathogenic Leishmania major parasite and mammalian actins share same overall fold co-polymerize with each other. Interestingly, harbors a simple actin-regulatory machinery that lacks cofilin ‘cofactors’, which accelerate filament disassembly in higher eukaryotes....
Fraxamoside, a macrocyclic secoiridoid glucoside featuring hydroxytyrosol group, was recently identified as xanthine oxidase inhibitor (XOI) comparable in potency vitro to the standard antigout drug allopurinol. However, this activity and its considerably higher value than derivatives oleuropein, oleoside 11-methyl ester, are not explained by structure-activity relationships (SARs) of known XOIs. To exclude allosteric mechanisms, we first determined inhibition kinetic fraxamoside. The...
Orchestrated action of peptidoglycan (PG) synthetases and hydrolases is vital for bacterial growth viability. Although the function several PG well understood, function, regulation, mechanism characterised as lysostaphin-like endopeptidases have remained elusive. Many these M23 family members can hydrolyse glycyl-glycine peptide bonds show lytic activity against Staphylococcus aureus whose contains a pentaglycine bridge, but their exact substrate specificity hydrolysed are still vaguely...
L-asparaginase from bacterial sources have been used in antineoplastic treatments and the food industry. A novel type II L-a sparaginase encoded by N-truncated gene ansZP21 of halotolerant Bacillus subtilis CH11 isolated Chilca salterns Peru was expressed using a heterologous system Escherichia coli BL21 (DE3)pLysS. The recombinant protein purified one-step nickel affinity chromatography exhibited activity 234.38 U mg-1 maximum catalytic at pH 9.0 60 °C. enzyme shows homotetrameric form with...
Abstract Orchestrated action of peptidoglycan (PG) synthetases and hydrolases is vital for bacterial growth viability. Although the function several PG well-understood, function, regulation, mechanism characterized as lysostaphin-like endopeptidases have remained elusive. Many these M23 family members can hydrolyse glycyl-glycine peptide bonds show lytic activity against Staphylococcus aureus whose contains a pentaglycine bridge, but their exact substrate specificity hydrolysed are still...
L-asparaginases from bacterial sources have been used in antineoplastic treatments and the food industry. A type II L-asparaginase encoded by N-truncated gene ansZP21 of halotolerant Bacillus subtilis CH11 isolated Chilca salterns Peru was expressed using a heterologous system Escherichia coli BL21 (DE3)pLysS. The recombinant protein purified one-step nickel affinity chromatography exhibited an activity 234.38 U mg−1 maximum catalytic at pH 9.0 60 °C. enzyme showed homotetrameric form with...
S. aureus resistance to antibiotics has increased rapidly. MRSA strains can simultaneously be resistant many different classes of antibiotics, including the so-called "last-resort" drugs. Resistance complicates treatment, increases mortality and substantially cost treatment. The need for new drugs against (multi)resistant is high. M23B family peptidoglycan hydrolases, enzymes that kill by cleaving glycine-glycine peptide bonds in cell wall are attractive targets drug development because...
Orchestrated action of peptidoglycan (PG) synthetases and hydrolases is vital for bacterial growth viability. Although the function several PG e.g., penicillin binding proteins well-understood, function, regulation, mechanism majority have remained elusive. Lysostaphin-like zinc-dependent metalloendopeptidases specifically hydrolyse glycyl-glycine peptide bond in notorious pathogen Staphylococcus aureus . In this work, we employed NMR spectroscopy to study substrate specificity...
Diseases caused by Leishmania and Trypanosoma parasites are a major health problem in tropical countries. Due to their complex life cycle involving both vertebrate insect hosts, >1 billion years of evolutionarily distance, the cell biology trypanosomatid exhibits pronounced differences animal cells. For example, actin cytoskeleton trypanosomatids is divergent when compared other eukaryotes. To understand how dynamics regulated parasites, we focused on central actin-binding protein profilin....
Orchestrated action of peptidoglycan (PG) synthetases and hydrolases is vital for bacterial growth viability. Although the function several PG well-understood, function, regulation, mechanism characterized as lysostaphin-like endopeptidases have remained elusive. Many these M23 family members can hydrolyse glycyl-glycine peptide bonds show lytic activity against Staphylococcus aureus whose contains a pentaglycine bridge, but their exact substrate specificity hydrolysed are still vaguely...
Orchestrated action of peptidoglycan (PG) synthetases and hydrolases is vital for bacterial growth viability. Although the function several PG well understood, function, regulation, mechanism characterised as lysostaphin-like endopeptidases have remained elusive. Many these M23 family members can hydrolyse glycyl-glycine peptide bonds show lytic activity against Staphylococcus aureus whose contains a pentaglycine bridge, but their exact substrate specificity hydrolysed are still vaguely...
Actin-rich cellular protrusions direct versatile biological processes from cancer cell invasion to dendritic spine development. The stability, morphology and specific function of these are regulated by crosstalk between three main signaling axes: integrins, actin regulators small GTPases. SHANK3 is a multifunctional scaffold protein, interacting with several actin-binding proteins, well-established autism risk gene. Recently, was demonstrated sequester integrin-activating GTPases Rap1 R-Ras...
Abstract Actin-rich cellular protrusions direct versatile biological processes from cancer cell invasion to dendritic spine development. The stability, morphology and specific function of these are regulated by crosstalk between three main signaling axes: integrins, actin regulators small GTPases. SHANK3 is a multifunctional scaffold protein, interacting with several actin-binding proteins, well-established autism risk gene. Recently, was demonstrated sequester integrin-activating GTPases...
Class I SH3 domain-binding motifs generally comply with the consensus sequence [R/K]xØPxxP, hydrophobic residue Ø being proline or leucine. We have studied unusual = Ala-specificity of SNX9 by determining its complex structure a peptide present in eastern equine encephalitis virus (EEEV) nsP3. The revealed length and composition n-Src loop as important factors specificity. also compared affinities EEEV nsP3 peptide, mutants, cellular ligands to SH3. These data suggest that has evolved...
ABSTRACT Diseases caused by Leishmania , and Trypanosoma parasites, such as leishmaniasis African sleeping sickness, are a major health problem in tropical countries. Due to their complex life cycle involving both vertebrate insect hosts, > 1 billion years of evolutionarily distance, the cell biology these trypanosomatid parasites exhibits pronounced differences animal cells. For example, actin cytoskeleton trypanosomatids is highly divergent when compared other eukaryotes. To understand...
ABSTRACT In hematopoietic tissues cell-cell communication involves immunoreceptors and specialized cell adhesion receptors that both mediate intracellular signals. Spleen tyrosine kinase (Syk) is a non-receptor involved in the downstream signaling of activation motif (ITAM) integrin family receptors. Both phosphorylated ITAM (pITAM) integrins bind to regulatory domain Syk composed two Src homology 2 (SH2) domains. The interaction with pITAM mediated by binding specific phosphotyrosine each...