Background: Oxidative stress affects sperm quality negatively.To maintain the pro/antioxidant balance, some metal ions (e.g.copper, zink, iron, selenium), which are co-factors of antioxidant enzymes, essential.However, iron and copper could act as prooxidants inducing oxidative damage spermatozoa.Aims: To reveal a possible correlation between concentrations (iron, copper, zinc, selenium) in human seminal plasma, stress, assessed by malondialdehyde total glutathione levels, semen quality,...

Stress and adaptation are inevitable components of the gymnastics training in competitive gymnasts. Salivary biomarkers, such as alpha-amylase activity (sAAA), concentration protein (sP) potassium (sK+) useful non-invasive stress indicators. The aim this study was to assess levels before competitions rhythmic gymnasts by using biochemical salivary markers anxiety questionnaires. included 10 national level (mean age: 14.7±1.57 years). Saliva collected salivates at three time points: nine days...

The difficult-to-heal wounds continue to be a problem for modern medicine. Chitosan and diosgenin possess anti-inflammatory antioxidant effects making them relevant substances wound treatment. That is why this work aimed study the effect of combined application chitosan on mouse skin model. For purpose, (6 mm diameter) were made mice’s backs treated 9 days with one following: 50% ethanol (control), polyethylene glycol (PEG) in ethanol, PEG (Chs), (Dg) chitosan, (ChsDg). Before first...

Abstract In vivo effects of N ‐benzyloxycarbonyl (Cbz)‐Leu–Leu‐leucinal (MG132) on chymotryptic‐like (ChT‐L), tryptic‐like, and post‐glutamyl peptide hydrolytic‐like proteasome activities, protein oxidation, lipid peroxidation (LP), glutathione (GSH) level, as well the activity antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), peroxidase (GSH‐Px), glutathione‐reductase) in rat liver were studied. The possibility MG132 provoking formation free oxygen radicals was also assayed...

Previous studies have shown that proteasome inhibitors exerted protective effects against ischemia/reperfusion injury (IRI) of brain, heart, kidney and intestine. The aim the present study was to investigate: (i) whether inhibitor MG132 protects rat liver IRI; (ii) modulates prooxidant/antioxidant status subjected warm IRI.The left lateral medial lobes (approximately 70% total volume) livers male Wistar rats were 30-min ischemia followed by 60-min reperfusion. Lactate dehydrogenase (LDH),...

Physical fitness has been shown to be a major health factor in both children and adolescents. One of the most widely applied physical test batteries Europe is Alpha-fit. However, there reference gap between some ages. The aim this study was propose interpolated percentile scores adolescents for main tests Alpha-fit battery, which can temporarily until filled by experimental research. recently available existing European normative values were linearly order close from 9.9 12.9 years age...

Rhythmic gymnastics is a sport in which special consideration given to diet and body appearance. The purpose of this study was assess the nutrient intake composition elite rhythmic gymnasts comparison with guidelines for female gymnasts. Twenty-one from Bulgaria were divided into three groups: First National Team, mean age 24.2; Second 17.8; Junior 12.6. Body fat muscle mass estimated by skinfold methods, food frequency questionnaire applied intake. showed low per cent (13.9% 17.0% 15.6%...

Background and Study Aim: One of the most widely applied methods to assess upper-body strength in children adolescents is handgrip test. While it has been determined which elbow position, type dynamometer are appropriate for achieving maximal grip strength, still not clear. Therefore, purpose this study was investigate whether position affect children. Material Methods: Grip measured by TKK DynX dynamometers, their validity reliability were also analysed. A total 60 children, 6 11 years old,...

The somatostatin analogs octreotide and lanreotide, selective to receptor subtypes 2 5, failed clinical efficacy for the prevention of restenosis after percutaneous transluminal angioplasty. These findings might have been result targeting a wrong subset receptors. In rat arteries, 1 4 are expressed 3-4 times more prominently than subtype is nearly exclusive in atherosclerotic human vessels. Here, we demonstrate that daily s.c. injections (50-500 microg/kg/d) CH275...

Abstract Copper toxicity is associated with formation of reactive oxygen species, which are capable to oxidize proteins. The selective removal the latter by 20S proteasome considered an essential part cell antioxidant defense system. aim present study was investigate whether peptidase activities rat liver proteasomes were affected chronic (40 mg CuSO 4 /rat/daily drinking water for 2 weeks) and acute (20 mg/kg , s.c.) copper treatment. To evaluate role proteasome, its inhibitor MG132 also...

Oxidative stress underlies the pathogenesis of many diseases, which determines interest in natural substances with antioxidant properties. Ginkgo biloba L. leaves are well known and widely used pharmaceutical industry, but therapeutic properties seeds less studied. This study aimed to identify chromatographic profile evaluate methanol extract from G. (GBSE). In GBSE, flavonoids terpenes were found as predominated. The GBSE capacity determined by 2,2 azino-bis...

Nowadays, an increased interest in natural compounds with preventive or therapeutic potential for various diseases has been observed. Given the involvement of oxidative stress pathogenesis gastric ulcer (GU) and wide range bioactive isolated from snails, this study aimed to investigate protective effect Cornu aspersum (Müller, 1774) mucus on ethanol-induced GUs. Male albino mice were divided into Control, Ethanol, Mucus + Ethanol Omeprazole treated groups. The GUs induced by administration...

Acute heart allograft rejection is characterized by leukocyte infiltration and myocyte damage, key elements in the histological grading of rejection. The induction selectins their ligands on graft postcapillary venular endothelium increases tethering to, rolling on, extravasation through into parenchyma. We have peptide mimicking selectin screening phage libraries using anti-Lewis A antibodies E-selectin as target molecules. effect this selectin- binding peptide, IELLQAR, prevention...

Background and Study Aim : Karate is going to take part in the Olympic games, for first time Tokyo 2020. The aim of this study was analyse changes heart rate (HR) blood lactate concentration karate practitioners performing different katas competitive conditions. Material Methods This consisted five elite male athletes (26.80±5.97 years), members Bulgarian national team Shotokan karate, competing kata discipline. conducted conditions during competitions, which three were performed by each...

Abstract The effects of nociceptin(1–13)NH2 (N/OFQ(1–13)NH2) and its structural analogue [Orn9]N/OFQ(1–13)NH2 on acute carrageenan (CG)-induced peripheral inflammation paw antioxidant status were studied. CG was injected intraplantarly in the right hind rats volume inflamed measured each 30 min for a period 4h. When administered simultaneously with CG, N/OFQ(1–13)NH2 decreased volume, whereas if 15 before it had no effect. produced opposite at same time-intervals administration. We also...

Abstract The production of reactive oxygen species (ROS) in cells is well balanced with their elimination by the antioxidant defence system. This balance essential for maintenance physiological conditions, and its disturbance (oxidative stress) has been suggested as a potential pathogenic mechanism variety diseases, accompanied inflammation. In this study, in-vivo effects nociceptin (N/OFQ(1–13)NH2) structure analogue [Orn9]N/OFQ(1–13)NH2 were studied on markers oxidative stress erythrocytes...

Abstract

Abstract In‐vivo effects of nociceptin (N/OFQ(1‐13)NH 2 ) on the levels lipid peroxidation and cell enzyme (superoxide dismutase, glutathione peroxidase reductase) non‐enzyme (glutathione) antioxidants in brain control kainic acid‐treated rats were studied. N/OFQ(1‐13)NH compared with those its structural analogue [Orn 9 ]N/OFQ(1‐13)NH . Kainic acid (25 µg, i.c.v) increased (4 24 h after treatment) decreased level (1 injection). We failed to find, any changes antioxidant activities,...

Young gymnasts should not have excessive body mass because that might lead to underperformance. Therefore, children practicing gymnastics consume a balanced diet, which will promote their physical abilities and optimal mass. The aim of this study was compare the nutrient intake young Bulgarian artistic matched groups from National Nutrition Survey in Bulgaria (NNSB2003). consisted 76 (5 14 years age) four cities Bulgaria, participants were divided into three accordance with age gender...

Successful performance in the Kumite discipline of Karate-do requires well developed aerobic and anaerobic cell-systems for supplying energy. Therefore, more often over recent years, preparation martial arts athletes has included intensive interval training sessions following Tabata method. The objective this survey was to create a highly-intensive Tabata-based circuit that is suitable functional Karate competitors defining physiological biochemical characteristics load through measuring...