A5041136996- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Antimicrobial Resistance in Staphylococcus
- Molecular Biology Techniques and Applications
- Bacteriophages and microbial interactions
- Entomopathogenic Microorganisms in Pest Control
- Insect Resistance and Genetics
- Insect and Pesticide Research
- Yersinia bacterium, plague, ectoparasites research
- Ecology and Vegetation Dynamics Studies
- Alcoholism and Thiamine Deficiency
- Biochemical and Structural Characterization
- Plant Micronutrient Interactions and Effects
- Trace Elements in Health
- Autoimmune Neurological Disorders and Treatments
- Clostridium difficile and Clostridium perfringens research
- Insect Pest Control Strategies
- Genomics and Phylogenetic Studies
- Iron Metabolism and Disorders
- Fire effects on ecosystems
- Forest ecology and management
Agrobiotechnology Institute
2017-2022
Gobierno de Navarra
2017-2022
Consejo Superior de Investigaciones Científicas
2022
Universidad Publica de Navarra
2019
Museo de Historia Natural Noel Kempff Mercado
2013
Background: The Amazon to Andes transition zone provides large expanses of relatively pristine forest wilderness across environmental gradients. Such elevational gradients are an excellent natural laboratory for establishing long-term interactions between ecosystems and parameters, which is valuable understanding ecosystem responses change. Aims: This study presents data on trends structure (biomass, basal area, height, stem density), species richness, composition from six transects in the...
RNA-binding proteins (RBPs) are essential to fine-tune gene expression. RBPs containing the cold-shock domain RNA chaperones that have been extensively studied. However, targets and specific functions for many of them remain elusive. Here, combining comparative proteomics RBP-immunoprecipitation-microarray profiling, we determined regulon chaperone CspA Staphylococcus aureus. Functional analysis revealed involved in carbohydrate ribonucleotide metabolism, stress response virulence expression...
Aedes albopictus is a species of mosquito, originally from Southeast Asia, that belongs to the Culicidae family and Dipteran insect order. The distribution this vector has rapidly changed over past decade, making most temperate territories in world vulnerable important human vector-borne diseases such as dengue, yellow fever, zika or chikungunya. Bacillus thuringiensis var. israeliensis (Bti)-based insecticides represent realistic alternative common synthetic for control mosquito larvae....
Abstract The evolution of gene expression regulation has contributed to species differentiation. 3′ untranslated regions (3′UTRs) mRNAs include regulatory elements that modulate expression; however, our knowledge their implications in the divergence bacterial is currently limited. In this study, we performed genome-wide comparative analyses encoding orthologous proteins from genus Staphylococcus and found mRNA conservation was lost mostly downstream coding sequence (CDS), indicating presence...
Thermoregulation of virulence genes in bacterial pathogens is essential for environment-to-host transition. However, the mechanisms governing cold adaptation when outside host remain poorly understood. Here, we found that production shock proteins CspB and CspC from Staphylococcus aureus controlled by two paralogous RNA thermoswitches. Through silico prediction, enzymatic probing site-directed mutagenesis, demonstrated cspB cspC 5'UTRs adopt alternative structures shift one another upon...
Bacterial genomes encode several families of protein paralogs. Discrimination between functional divergence and redundancy among paralogs is challenging due to their sequence conservation. Here, we investigated whether the amino acid differences present in cold shock (CSP) Staphylococcus aureus were responsible for specificity. Since deletion cspA reduces synthesis staphyloxanthin (STX), used it as an vivo reporter CSP functionality. Complementation a ΔcspA strain with different S. variants...
Bacterial genomes are pervasively transcribed, generating a wide variety of antisense RNAs (asRNAs). Many them originate from transcriptional read-through events (TREs) during the transcription termination process. Previous transcriptome analyses revealed that lexA gene Staphylococcus aureus, which encodes main SOS response regulator, is affected by presence an asRNA. Here, we show RNA (lexA-asRNA) generated TRE on intrinsic terminator (TTsbrB) sbrB gene, located downstream lexA, in opposite...
Chemical products still represent the most common form of controlling crop pests and diseases. However, their extensive use has led to selection resistances. This makes finding new solutions paramount countering economic losses that diseases in modern agriculture. Bacillus thuringiensis (Bt) is one reliable alternatives chemical-based solutions. In this study, we aimed further expand global applicability Bt strains beyond spores crystals. To end, selected a strain (BST-122) with relevant...
Molecular beacons (MBs) are oligonucleotide probes with a hairpin-like structure that typically labelled at the 5′ and 3′ ends fluorophore quencher dye, respectively. The conformation of MB acts as switch for fluorescence emission. When is in close proximity to quencher, emission cannot be detected, meaning an OFF state. However, if modified, separating from turns ON allowing This property has been extensively used wide variety applications including real-time PCR reactions, study...
ABSTRACT Pathogenic bacteria must rapidly adapt to ever-changing environmental signals or nutrient availability resulting in metabolism remodeling. The carbon catabolite repression represents a global regulatory system, allowing the express genes involved utilization and metabolization of preferred source. In Staphylococcus aureus , regulation repressing is mediated by protein A (CcpA). Here, we have identified CcpA-dependent small non-coding RNA, RsaI that inhibited high glucose...
This is part 3.1 of the "Fluorescent Molecular Beacons Mimicking RNA Secondary Structures to Study Chaperone Activity" collection protocols. Collection Abstract: beacons (MBs) are oligonucleotide probes with a hairpin-like structure that typically labelled at 5' and 3' ends fluorophore quencher dye, respectively. The conformation MB acts as switch for fluorescence emission. When in close proximity quencher, emission cannot be detected, meaning an OFF state. However, if modified, separating...
This is part 3.2 of the "Fluorescent Molecular Beacons Mimicking RNA Secondary Structures to Study Chaperone Activity" collection protocols. Collection Abstract: beacons (MBs) are oligonucleotide probes with a hairpin-like structure that typically labelled at 5' and 3' ends fluorophore quencher dye, respectively. The conformation MB acts as switch for fluorescence emission. When in close proximity quencher, emission cannot be detected, meaning an OFF state. However, if modified, separating...
Molecular beacons (MBs) are oligonucleotide probes with a hairpin-like structure that typically labelled at the 5' and 3' ends fluorophore quencher dye, respectively. The conformation of MB acts as switch for fluorescence emission. When is in close proximity to quencher, emission cannot be detected, meaning an OFF state. However, if modified, separating from turns ON allowing This property has been extensively used wide variety applications including real-time PCR reactions, study...
Iron acquisition and modulation of its intracellular concentration are critical for the development all living organisms. So far, several proteins have been described to be involved in iron homeostasis. Among them, ferritins act as major storage proteins, sequestering internalized modulating inside bacterial cells. We previously that deletion 3’-untranslated region (3’UTR) ftnA gene, which codes ferritin Staphylococcus aureus , increased mRNA levels. Here, we show levels affected by RNase...