In living systems, subcellular organelles mutually cooperate and closely contact to form organelle interaction networks. Thus, the simultaneous discriminative visualization of different is extremely valuable for elucidating their distribution interplay. However, such meaningful investigations remain a great challenge due lack advanced single fluorescent probes (SF-probes) capable two-color imaging two targets. Herein, first time, we present excited-state intramolecular proton transfer...

We present two mitochondria-immobilized fluorescent probes ECPI-12 and IVPI-12 for long-term mitochondria visualization tracking regardless of MMP changes.

Herein, we reported a red-emitting probe (E)-4-(2-(8-hydroxy-julolidine-9-yl)vinyl)-1-methylpyridin-1-ium iodide (HJVPI) on rotor mechanism with an ultrahigh signal-to-noise ratio. HJVPI could give high-fidelity fluorescent images of mitochondria in living immortalized and normal cells be suitable for IR excitation source two-photon microscopy various sources confocal microscopy. As rotor, its single/two-photon fluorescence intensities directly depended environmental viscosity. And, as...

Lipid droplets (LDs) with unique interfacial architecture not only play crucial roles in protecting a cell from lipotoxicity and lipoapoptosis but also closely relate many diseases such as fatty liver diabetes. Thus, one of the important applied biomaterials, fluorescent probes ultrahigh selectivity for situ high-fidelity imaging LDs living cells tissues are critical to elucidate relevant physiological pathological events well detect related diseases. However, available utilizing LDs'...

The feedback from mitochondrial membrane potential (MMP) in different situations (normal, decreasing, and vanishing) can reflect cellular status, which be applied biomedical research diagnosis of the related diseases. Thus, efficient convenient detection for MMP is particularly important, yet operations current fluorescent probes are complex. In order to address this concern, we presented herein a spatially dependent probe composed organic cationic salt. experimental results normal...

To gain insights into the coupling of conformational and electronic variables, we exploited steric hindrance to modulate a polycyclic skeleton with bent conformation in S0 state twisted S1 under guidance photoexcited aromaticity reversals. Polycyclic 5,10-dihydrophenazine (DHP) adopted structure but involved bent-to-planar transformation due excited-state 8π-electron central ring. The N,N'-locations 1,4,6,9-sites DHP provided versatile chemical handle for fine-tuning intramolecular...

A side-chain engineering strategy can dramatically improve dye delivery by fine-tuning the adsorption and desorption abilities of transmembrane process, enabling ultralow loading bioimaging deep tissue penetration.

The viscosity distribution of micellar interiors from the very center to outer surface is dramatically varied, which has been distinguished in theoretical models, yet it remains highly challenging quantify this issue experimentally. Herein, a series fluorophore-substituted surfactants DPAC-Fn (n = 3, 5, 7, 9, 11, 13, and 15) are developed by functionalizing different alkyl-trimethylammonium bromides with butterfly motion-based sensor, N,N'-diphenyl-dihydrodibenzo[a,c]phenazine (DPAC)....

Mitochondria membrane potential (MMP) play significant roles during metabolism, signaling, and other important bioevents. Visualization of MMP levels is essential for many biological researches. However, fluorescent probes monitoring in dual emission colors are still deficient, which greatly limited the development relative research areas. In this work, a pair have been designed synthesized to monitor based on Forster resonance energy transfer (FRET) mechanism. The FRET donor (FixD)...

The mitochondrial membrane potential (MMP) definitely reflects function. Thus, it is very essential to found a physical parameter as MMP indicator. At present, available parameters are either fluorescent intensity of monochromatic probes such rhodamine 123 or ratio at different wavelengths dual-color dyes JC-1, but the inconvenience in practice well serious effect loading concentrations on experimental results limited their application. To address this concern, herein,we reliable and easily...

In situ and directly imaging mitochondria in tissues instead of isolated cells can offer more native accurate information. Particularly, the clinical diagnose mitochondrial diseases such as myopathy, it is a routine examination item to observe morphology number muscle from patients. However, still challenging task because selectivity available probes inadequate for exclusively tissue imaging. Inspired by chemical structure amphiphilic phospholipids inner membrane, we synthesized...

Based on styrylpyridine salts, a small-molecule red emitting membrane probe with large two-photon absorption cross-section has been synthesized. As molecular rotor, it enables exclusive lighting up of the plasma in live cells and particular tissues. This potential to be powerful tool for bioimaging.

Ferroptosis is a new mode of cell death with major morphological changes in mitochondria, including structural shrinkage and increased membrane density, indicating the mitochondrial abnormality during this process. Viscosity, as one crucial microenvironmental parameters for characterizing state, thought to be highly involved ferroptosis. Herein, we present single fluorescent probe (PPAC-C4) dual ratio ultrahigh-accuracy quantification viscosity. This constructed by linking...

Myelodysplastic syndromes (MDS) are a heterogeneous group of clonal stem cell disorders characterized by cytopenia and dysplasia. Anemia is the most common symptom in patients with MDS. Mitophagy mitochondrial dysfunction might be involved development In this study, we investigated change mitophagy erythroid precursors MDS patients. We found that NIX‐mediated was impaired bone marrow nucleated red blood cells (NRBC) patients, associated an increased amount damaged mitochondria ROS level...

Solvatochromic fluorescent probes are crucial molecular tools to investigate and aggregate proteins' fold, visualize fine structures in biomembranes, label different organelles dual emission colors. However, solvatochromic fluorogens often displayed a weak at high polarity, hindering their bioimaging applications. To resolve this problem, herein, we propose an intramolecular charge transfer (ICT) inhibition strategy. The probe was designed with single electronic donor two acceptors order...

The development of two-photon fluorophores remains an important issue. Dyes that possess both large excited fluorescence action cross-sections and cell membrane permeability are especially in demand to maximize the underlying virtue microscopy for bioimaging. Herein, a novel dye has been synthesized. This V-shaped exhibited high plasma permeability. Cell imaging experiments demonstrated could stain living cells with bright fluorescence. All results have indicated potential as basic platform probes.