A5104011230- Nanoplatforms for cancer theranostics
- Photodynamic Therapy Research Studies
- Photoacoustic and Ultrasonic Imaging
- Extracellular vesicles in disease
- Nanoparticle-Based Drug Delivery
- Biosensors and Analytical Detection
- Advanced Biosensing Techniques and Applications
- Cancer Cells and Metastasis
- Bone health and treatments
- Cancer Research and Treatments
- Ovarian cancer diagnosis and treatment
- Lanthanide and Transition Metal Complexes
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Optical Imaging and Spectroscopy Techniques
- TGF-β signaling in diseases
- Advanced biosensing and bioanalysis techniques
- Luminescence Properties of Advanced Materials
- MicroRNA in disease regulation
- HER2/EGFR in Cancer Research
- RNA Interference and Gene Delivery
- Infant Health and Development
- Bone Metabolism and Diseases
- Spectroscopy Techniques in Biomedical and Chemical Research
- RNA regulation and disease
Rutgers, The State University of New Jersey
2013-2024
Alex's Lemonade Stand Foundation
2024
Rutgers Cancer Institute of New Jersey
2010-2012
Abstract Background Transforming Growth Factor β (TGF-β) plays an important role in tumor invasion and metastasis. We set out to investigate the possible clinical utility of TGF-β antagonists a human metastatic basal-like breast cancer model. examined effects two types pathway (1D11, mouse monoclonal pan-TGF-β neutralizing antibody LY2109761, chemical inhibitor type I II receptor kinases) on sublines basal cell-like MDA-MB-231 carcinoma cells that preferentially metastasize lungs (4175TR,...
As a nascent and emerging field that holds great potential for precision oncology, nanotechnology has been envisioned to improve drug delivery imaging capabilities through precise efficient tumor targeting, safely sparing healthy normal tissue. In the clinic, nanoparticle formulations such as first-generation Abraxane® in breast cancer, Doxil® sarcoma, Onivyde® metastatic pancreatic have shown advancement while improving safety profiles. However, effective accumulation of nanoparticles at...
Realizing the promise of precision medicine in cancer therapy depends on identifying and tracking cancerous growths to maximize treatment options improve patient outcomes. This goal early detection remains unfulfilled by current clinical imaging techniques that fail detect lesions due their small size suborgan localization. With proper probes, optical can overcome this molecular phenotype tumors at both macroscopic microscopic scales. In study, first use nanophotonic short wave infrared...
We have used the human folate transporter cDNA and a genomic clone hybridizing to perform chromosomal mapping of gene. Human-rodent somatic cell hybrid analysis using as probe revealed perfect segregation with chromosome 21. In situ hybridization metaphase spreads mapped gene distal long arm 21, band q22.3. This location was confirmed by fluorescence in clone.
Therapeutic drug monitoring (TDM) in cancer, while imperative, has been challenging due to inter-patient variability pharmacokinetics. Additionally, most pharmacokinetic is done by assessments of the drugs plasma, which not an accurate gauge for concentrations target tumor tissue. There exists a critical need therapy tools that can provide real-time feedback on efficacy at site enable alteration treatment regimens early during cancer therapy. Here, we report theranostic optical imaging...
Rare-earth (RE) doped nanocomposites emit visible luminescence when illuminated with continuous wave near-infrared light, making them appealing candidates for use as contrast agents in biomedical imaging. However, the emission lifetime of these materials is much longer than pixel dwell times used scanning intravital microscopy. To overcome this limitation, we have developed a line-scanning confocal microscope high-resolution, optically sectioned imaging samples labeled RE-based...
Gene therapy is emerging as the next generation of therapeutic modality with United States Food and Drug Administration approved gene-engineered for cancer a rare eye-related disorder, but challenge real-time monitoring on-target response remains. In this study, we have designed theranostic nanoparticle composed shortwave-infrared-emitting rare-earth-doped nanoparticles (RENPs) capable delivering genetic cargo monitoring. We showed that cationic coating RENPs branched polyethylenimine (PEI)...
Abstract Background The ability to detect tumor-specific biomarkers in real-time using optical imaging plays a critical role preclinical studies aimed at evaluating drug safety and treatment response. In this study, we engineered an platform capable of targeting different tumor multi-colored library nanoprobes. These probes contain rare-earth elements that emit light the short-wave infrared (SWIR) wavelength region (900–1700 nm), which exhibits reduced absorption scattering compared visible...
Rare-earth-doped nanocomposites have appealing optical properties for use as biomedical contrast agents, but few systems exist imaging these materials. We describe the design and characterization of (i) a preclinical system whole animal in vivo (ii) an integrated coherence tomography/confocal microscopy high-resolution ex tissues. demonstrate by administering erbium-doped to murine model metastatic breast cancer. Short-wave infrared emissions were detected organ vivo. Visible upconversion...
Background Late-stage diagnosis of ovarian cancer, a disease that originates in the ovaries and spreads to peritoneal cavity, lowers 5-year survival rate from 90% 30%. Early screening tools can: i) detect with high specificity sensitivity before conventional such as transvaginal ultrasound CA-125, ii) use non-invasive sampling methods iii) longitudinally significantly increase rates cancer are needed. Studies employ blood-based using circulating tumor-cells, -DNA, most recently tumor-derived...
We report the design, calibration, and testing of a pre-clinical small animal imaging platform for use with short-wave infrared (SWIR) emitting contrast agents. Unlike materials at visible or near-infrared wavelengths, SWIR-emitting agents require detection systems sensitivity in 1-2 μm wavelength region, beyond range commercially available imagers. used collimated 980 nm laser beam to excite rare-earth-doped NaYF<sub>4</sub>:Er,Yb nanocomposites, as an example SWIR material under...
Checkpoint immunotherapy has made great strides in the treatment of solid tumors, but many patients do not respond to immune checkpoint inhibitors. Identification tumor‐infiltrating cytotoxic T cells (CTLs) potential stratify and monitor responses. In this study, design cluster differentiation (CD8 + ) cell‐targeted nanoprobes that emit shortwave infrared (SWIR) light second tissue‐transparent window for noninvasive, real‐time imaging CTLs murine models breast cancer is presented....
Abstract Although triple-negative breast cancer (TNBC) can be treated with anti-PD-1 checkpoint immunotherapy in combination chemotherapy, there remains a challenge effectively monitoring therapeutic responses. Current non-invasive clinical imaging tools to evaluate response treatment are reliant upon measurements of tumor volume and may fail distinguish true progression from increased immune cell infiltration. Invasive biopsy sampling immunohistochemistry (IHC) elucidate changes the...
Metastatic breast cancer remains a significant source of mortality amongst patients and is generally considered incurable in part due to the difficulty detection early micro-metastases. The pre-metastatic niche (PMN) tissue microenvironment that has undergone changes support colonization growth circulating tumor cells, key component which myeloid-derived suppressor cell (MDSC). Therefore, MDSC been identified as potential biomarker for PMN formation, would enable clinicians proactively treat...
Abstract Metastatic cancer is the leading cause of almost 90% cancer-related deaths. Early detection metastatic lesions in breast would lead to judicious therapeutic choices management disease. Current clinical imaging techniques are unable detect micro-metastases due limited resolution. Optical technologies challenged by high tissue scattering and absorption visible light, limiting resolution deeper lesions. While optical modalities have potential for real-time vivo monitoring, poor...
Fluorescence-guided surgery (FGS) is an emerging technique for tissue visualization during surgical procedures. Structures of interest are labeled with exogenous probes whose fluorescent emissions acquired and viewed in real-time optical imaging systems. This study investigated rare-earth-doped albumin-encapsulated nanocomposites (REANCs) as short-wave infrared emitting contrast agents FGS. Experiments were conducted using animal model 4T1 breast cancer. The signal-to-background ratio (SBR)...
We are investigating the ability of targeted rare earth (RE) doped nanocomposites to detect and track micrometastatic breast cancer lesions distant sites in pre-clinical vivo models. Functionalizing RE with AMD3100 promotes targeting CXCR4, a recognized marker for highly metastatic disease. Mice were inoculated SCP-28 (CXCR4 positive) 4175 negative) cell lines. Whole animal SWIR fluorescence imaging was performed after bioluminescence confirmed tumor burden lungs. Line-scanning confocal...
Event Abstract Back to Cancer-targeted short-wave infra-red emitting rare-earth albumin nanocomposites for lesion mapping and deep-tissue imaging Margot Zevon1, Vidya Ganapathy1, Harini Kantamneni1, Laura Higgins1, Marco P. Mingozzi1, Richard E. Riman2, Charles M. Roth1, Mark Pierce1 Prabhas Moghe1 1 Rutgers University, Biomedical Engineering, United States 2 Materials Science Introduction: Success of personalized medicine in cancer therapy depends on the ability molecularly phenotype...
We describe the development and testing of a line-scanning confocal microscope integrated / optical coherence tomography system for imaging biological cells tissues labeled with molecularly-targeted rare-earth-doped nanocomposites.
Success of personalized medicine in cancer therapy depends on the ability to identify and molecularly phenotype tumors. Current clinical imaging techniques cannot be integrated with precision molecular at level single cells or microlesions due limited resolution. In this work we use targeted infrared emitting optical probes characterize metastatic prior their detection clinically relevant modalities. These contrast agents form basis an <i>in vivo</i> system capable resolving internal...