A5042966626- Immune Response and Inflammation
- Complement system in diseases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cell Adhesion Molecules Research
- Sepsis Diagnosis and Treatment
- S100 Proteins and Annexins
- Blood Coagulation and Thrombosis Mechanisms
- Neonatal Respiratory Health Research
- Respiratory Support and Mechanisms
- Macrophage Migration Inhibitory Factor
- Nitric Oxide and Endothelin Effects
- Inflammasome and immune disorders
- Heme Oxygenase-1 and Carbon Monoxide
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cardiac Ischemia and Reperfusion
- Monoclonal and Polyclonal Antibodies Research
- Immune cells in cancer
- Inflammatory mediators and NSAID effects
- Inflammation biomarkers and pathways
- Chemokine receptors and signaling
- Antibiotics Pharmacokinetics and Efficacy
- Receptor Mechanisms and Signaling
- Immune Cell Function and Interaction
- Eicosanoids and Hypertension Pharmacology
- Erythrocyte Function and Pathophysiology
University of Warwick
2025
Dorset County Hospital
2025
University of Michigan
2015-2024
Massachusetts General Hospital
1973-2024
The University of Texas at Austin
1996-2023
Royal North Shore Hospital
1997-2023
Google (United States)
2022
British Dental Association
2019
Ann Arbor Center for Independent Living
1994-2018
British Antarctic Survey
1996-2017
Neutrophil-endothelial cell interactions are mediated by interacting sets of adhesion molecules (CAMs) and chemoattractant/activator to form an “adhesion cascade.” The initial phase inflammation, a transient slowing neutrophils in postcapillary venules, is selectins. Subsequently, firm the vessel wall occurs via interaction CD11/GD18 (β2) integrins endothelial ligands such as intercellular molecule-1 (ICAM-1). This binding requires activation exposure neutrophil variety...
Human endothelial cells produced a neutrophil chemotactic factor (NCF) upon stimulation with tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), or lipopolysaccharide (LPS). The expression of cell-derived NCF messenger RNA and biological activity was both time- concentration-dependent. Maximal mRNA occurred at 10 2 nanograms per milliliter for TNF IL-1β, respectively; first observed 1 hour after maintained least 24 hours. In situ hybridization analysis showed that peaked in treated by...
In the present paper, we characterize an antibody, mAb BV13, directed to mouse vascular endothelial (VE)-cadherin, a major adhesive protein of interendothelial adherens junctions. When added cultured cells, BV13 induces redistribution VE-cadherin from intercellular did not change localization platelet cell adhesion molecule or tight junction markers such as zonula occludens 1, cingulin, and junctional molecule. Intravenous administration induced concentration- time-dependent increase in...
Intravascular activation of the complement system with cobra venom factor results in acute lung injury, which has been quantitated by increases vascular permeability. Cobra preparations devoid phospholipase A2 activity retain full lung-damaging capacity. The injury is associated preceding appearance chemotactic serum coincident development a profound neutropenia. immunochemically related to human C5a. Morphologic studies have revealed discontinuities endothelial cell lining alveolar...
Chemotactic factor-enriched butanol extracts from Escherichia coli culture filtrates were fractionated and purified by high pressure liquid chromatography. The yield individual fractions of biological activity (lysosomal enzyme secretion) antigenic (competition with [3H]fMet-Leu-Phe for binding to rabbit anti-fMet-Leu-Phe) revealed an average 50% recovery original material. Five peaks separated as demonstrated enzyme-releasing activity. Three these coincided exactly activity, suggesting that...
Sepsis remains a serious cause of morbidity and mortality, the pathophysiology disease is not clear. The definition clinical manifestations sepsis ever evolving. This review discusses search for effective therapeutic interventions, hurdles in translational research, new therapies development current trials.
Human umbilical vein endothelial cells have recently been shown to respond C5a with increases in intracellular Ca2+, production of D-myo-inositol 1,4,5-triphosphate and superoxide anion generation. In the current studies, had found cause a time- dose-dependent manner rapid expression P-selectin, secretion von Willebrand factor, adhesiveness for human neutrophils. The effects P-selectin neutrophils were similar histamine thrombin on cells. C5a-stimulated endothelium was blocked by...
During inflammation, neutrophils migrate from the vascular lumen into extravascular sites. In vitro assays have suggested that platelet-endothelial cell adhesion molecule-1 [PECAM-1 (CD31)], a member of immunoglobulin superfamily, is required for transmigration across endothelial monolayers. Antibody to human PECAM-1, which cross-reacts with rat was found block not only in vivo accumulation peritoneal cavity and alveolar compartment lung but also neutrophil skin grafts transplanted onto...