- Viral Infectious Diseases and Gene Expression in Insects
- Insect Resistance and Genetics
- Invertebrate Immune Response Mechanisms
- Insect symbiosis and bacterial influences
- Insect Utilization and Effects
- Insect behavior and control techniques
- Entomopathogenic Microorganisms in Pest Control
- Neurobiology and Insect Physiology Research
- Animal Virus Infections Studies
- Insect and Pesticide Research
- Mosquito-borne diseases and control
- Virus-based gene therapy research
- Silkworms and Sericulture Research
- Plant Virus Research Studies
- Viral Infections and Immunology Research
- Insect-Plant Interactions and Control
- RNA Interference and Gene Delivery
- Silk-based biomaterials and applications
- Autophagy in Disease and Therapy
- Antimicrobial Peptides and Activities
- Environmental Toxicology and Ecotoxicology
- Studies on Chitinases and Chitosanases
Anhui Agricultural University
2021-2024
Southwest University
2015-2020
Abstract MicroRNAs (miRNAs) are small non‐coding RNAs that play pivotal roles in the host response to invading pathogens. Among these pathogens, Bombyx mori nucleopolyhedrovirus (BmNPV) is one of main causes substantial economic losses sericulture, and there relatively few studies on specific functions miRNAs B. –BmNPV interaction. Therefore, we conducted transcriptome sequencing identify differentially expressed (DE) messenger (mRNAs) midgut 2 strains (BmNPV‐susceptible strain P50...
The oriental fruit fly, Bactrocera dorsalis (Hendel), is one of the most destructive agricultural pests and hosts diverse intestinal bacterial communities. We used 16S rRNA gene sequencing to investigate microbial communities associated with intestines females males from two B. populations collected Hainan Guizhou Provinces China. A total 260,615 high-quality reads an average length 253 bp were obtained. Highly observed across individuals, containing between 691 1,262 operational taxonomic...
The oriental fruit fly, Bactrocera dorsalis (Hendel), is an important agricultural pest and has developed resistance to many insecticides. To investigate vital genes participating in metamorphosis for development of additional control strategies, a comprehensive transcriptome analysis covering ten developmental stages B. was performed.There were 2132, 952, 1062, 2301 1333 differentially expressed identified during hatching, 1st-instar larval molting, 2nd-instar pupariation emergence,...
The RNA interference pathway mediated by microRNAs (miRNAs) is one of the methods to defend against viruses in insects. Recent studies showed that miRNAs participate viral infection binding target genes regulate their expression. Here, we found Bombyx mori miRNA, miR-6498-5p was down-regulated, whereas its predicted gene pyridoxal phosphate phosphatase PHOSPHO2 (BmPLPP2) up-regulated upon nucleopolyhedrovirus (BmNPV) infection. Both vivo and vitro experiments targets BmPLPP2 suppresses...
β-1,3-glucan recognition proteins (βGRPs) as pattern receptors (PRRs) play an important role in recognizing various pathogens and trigger complicated signaling pathways insects. In this study, we identified a Bombyx mori protein gene named BmβGRP4, which showed differential expression, from previous transcriptome database. The full-length cDNA sequence was 1244 bp, containing open reading frame (ORF) of 1128 bp encoding 375 amino acids. BmβGRP4 strongly expressed the larval stages highly...
Abstract Bombyx mori L. (Lepidoptera: Bombycidae) nucleopolyhedrovirus (BmNPV) is a serious pathogen causing huge economic losses to sericulture. There growing evidence that the gut microbiota of silkworms plays critical role in shaping host responses and interactions with viral infection. However, little known about differences composition diversity intestinal microflora, especially respect silkworm strain BmNPV infection-induced changes. Here, we aim explore between BmNPV-resistant A35...
The citrus red mite, Panonychus citri, a major pest distributed worldwide, has evolved severe resistance to various classes of chemical acaricides/insecticides including pyrethroids. It is well known that the pyrethroids mainly caused by point mutations voltage-gated sodium channel gene in wide range pests. However, increasing number evidences support might also be resulted from integrated mechanisms metabolic mechanisms. In this study, firstly, comparative analysis RNA-seq data showed...
The oriental fruit fly (Bactrocera dorsalis) is a pest that causes large economic losses in the and vegetable industry, so its control major challenge. Nuclear receptors (NRs) are superfamily of ligand-dependent transcription factors directly combine with DNA to regulate expression downstream target genes. NRs closely associated multiple physiological processes such as metabolism, reproduction, development. Through sequence searches analysis, we identified 21 B. dorsalis NR genes, all which...
Phormicins belong to defensin family, which are important antimicrobial peptides (AMPs) in insects. These AMPs inducible upon challenging by immune triggers. In the present study, we identified cDNA of a phormicin gene (BdPho) oriental fruit fly, Bactrocera dorsalis (Hendel), ruinous agricultural pest causing great economic losses fruits and vegetables. The BdPho contains 282 bp open reading frame encoding 93 amino acid residues, predicted molecular weight isoelectric point peptide were 9.83...
Ferritin heavy chain (FerHCH) is a major component of ferritin and plays an important role in maintaining iron homeostasis redox equilibrium. Our previous studies have demonstrated that the Bombyx mori homolog (BmFerHCH) could respond to B. nucleopolyhedrovirus (BmNPV) infection. However, mechanism by which BmNPV regulates expression BmFerHCH remains unclear. In this study, increased after infection enhanced nuclear factor kappa B (NF-κB) activity BmN cells. An NF-κB inhibitor (PDTC) reduced...
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