A5001112482- Neurobiology and Insect Physiology Research
- Insect and Arachnid Ecology and Behavior
- Physiological and biochemical adaptations
- Olfactory and Sensory Function Studies
- Neural dynamics and brain function
- Animal Behavior and Reproduction
- Plant and animal studies
- Insect Utilization and Effects
- Genetics, Aging, and Longevity in Model Organisms
- Insect Pheromone Research and Control
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Antioxidant Activity and Oxidative Stress
Carleton College
2023-2024
University of Puerto Rico, Medical Sciences Campus
2023-2024
Janelia Research Campus
2017-2022
Howard Hughes Medical Institute
2017-2022
Helix (United States)
2020
Drosophila melanogaster has a rich repertoire of innate and learned behaviors. Its 100,000-neuron brain is large but tractable target for comprehensive neural circuit mapping. Only electron microscopy (EM) enables complete, unbiased mapping synaptic connectivity; however, the fly too conventional EM. We developed custom high-throughput EM platform imaged entire an adult female at resolution. To validate dataset, we traced brain-spanning circuitry involving mushroom body (MB), which been...
In pursuit of food, hungry animals mobilize significant energy resources and overcome exhaustion fear. How need motivation control the decision to continue or change behavior is not understood. Using a single fly treadmill, we show that flies persistently track food odor increase their effort over repeated trials in absence reward suggesting dominates negative experience. We further tracking regulated by two mushroom body output neurons (MBONs) connecting MB lateral horn. These MBONs,...
Associative memory formation and recall in the fruit fly Drosophila melanogaster is subserved by mushroom body (MB). Upon arrival MB, sensory information undergoes a profound transformation from broadly tuned stereotyped odorant responses olfactory projection neuron (PN) layer to narrowly nonstereotyped Kenyon cells (KCs). Theory experiment suggest that this implemented random connectivity between KCs PNs. However, hypothesis has been challenging test, given difficulty of mapping synaptic...
SUMMARY Drosophila melanogaster has a rich repertoire of innate and learned behaviors. Its 100,000–neuron brain is large but tractable target for comprehensive neural circuit mapping. Only electron microscopy (EM) enables complete, unbiased mapping synaptic connectivity; however, the fly too conventional EM. We developed custom high-throughput EM platform imaged entire an adult female fly. validated dataset by tracing brain-spanning circuitry involving mushroom body (MB), intensively studied...
Abstract Associative memory formation and recall in the adult fruit fly Drosophila melanogaster is subserved by mushroom body (MB). Upon arrival MB, sensory information undergoes a profound transformation. Olfactory projection neurons (PNs), main MB input, exhibit broadly tuned, sustained, stereotyped responses to odorants; contrast, their postsynaptic targets Kenyon cells (KCs), are nonstereotyped, narrowly only briefly responsive odorants. Theory experiment have suggested that this...
Serotonergic neurons project widely throughout the brain to modulate diverse physiological and behavioral processes. However, a single-cell resolution understanding of connectivity serotonergic is currently lacking. Using whole-brain EM dataset female <i>Drosophila</i>, we comprehensively determine wiring logic broadly projecting neuron (the CSDn) that spans several olfactory regions. Within antennal lobe, CSDn differentially innervates each glomerulus, yet surprisingly, this variability...
Mechanosensory neurons located across the body surface respond to tactile stimuli and elicit diverse behavioral responses, from relatively simple stimulus location-aimed movements complex movement sequences. How mechanosensory their postsynaptic circuits influence such behaviors remains unclear. We previously discovered that Drosophila perform a location-prioritized grooming sequence when at different locations on head are simultaneously stimulated by dust (Hampel et al., 2017; Seeds 2014)....
Mechanosensory neurons located across the body surface respond to tactile stimuli and elicit diverse behavioral responses, from relatively simple stimulus location-aimed movements complex movement sequences. How mechanosensory their postsynaptic circuits influence such behaviors remains unclear. We previously discovered that Drosophila perform a location-prioritized grooming sequence when at different locations on head are simultaneously stimulated by dust (Hampel et al., 2017; Seeds 2014)....
Serotonin plays different roles across networks within the same sensory modality. Previously, we used whole-cell electrophysiology in Drosophila to show that serotonergic neurons innervating first olfactory relay are inhibited by odorants (Zhang and Gaudry, 2016). Here network-spanning segregate information about stimulus features, odor intensity identity, using opposing coding schemes neuropil. A pair of (the CSDns) innervate antennal lobe lateral horn, which second order neuropils. CSDn...
Mechanosensory neurons located across the body surface respond to tactile stimuli and elicit diverse behavioral responses, from relatively simple stimulus location-aimed movements complex movement sequences. How mechanosensory their postsynaptic circuits influence such behaviors remains unclear. We previously discovered that
Mechanosensory neurons located across the body surface respond to tactile stimuli and elicit diverse behavioral responses, from relatively simple stimulus location-aimed movements complex movement sequences. How mechanosensory their postsynaptic circuits influence such behaviors remains unclear. We previously discovered that Drosophila perform a location-prioritized grooming sequence when at different locations on head are simultaneously stimulated by dust (Hampel et al., 2017; Seeds 2014)....
In pursuit of palatable food, hungry animals mobilize significant energy resources and overcome obstacles, exhaustion fear. Their perseverance depends on metabolic state, internal motivation the expected benefit. Sustained commitment to a trying task is crucial, however, disengagement from one behavior engage into another can be essential for optimal adaptation survival. How neural circuits allow prioritizing over withdrawal based animal's need, not understood. Using single fly spherical...
Mechanosensory neurons located across the body surface respond to tactile stimuli and elicit diverse behavioral responses, from relatively simple stimulus location-aimed movements complex movement sequences. How mechanosensory their postsynaptic circuits influence such behaviors remains unclear. We previously discovered that Drosophila perform a location-prioritized grooming sequence when at different locations on head are simultaneously stimulated by dust (Hampel et al., 2017; Seeds 2014)....
Abstract Serotonergic neurons modulate diverse physiological and behavioral processes in a context-dependent manner, based on their complex connectivity. However, connectivity has not been comprehensively explored at single-cell resolution. Using whole-brain EM dataset we determined the wiring logic of broadly projecting serotonergic neuron (the “CSDn”) Drosophila . Within antennal lobe (AL; first-order olfactory region), CSDn receives glomerulus-specific input preferentially targets...