A5036742303- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Reproductive Health and Technologies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Hormonal and reproductive studies
- Prostate Cancer Treatment and Research
- Ovarian function and disorders
- Renal and related cancers
- Animal Genetics and Reproduction
- Reproductive biology and impacts on aquatic species
- Estrogen and related hormone effects
- Selenium in Biological Systems
- Cancer, Lipids, and Metabolism
- Prostate Cancer Diagnosis and Treatment
- Ubiquitin and proteasome pathways
- Effects and risks of endocrine disrupting chemicals
- Toxoplasma gondii Research Studies
- Reproductive System and Pregnancy
- Urological Disorders and Treatments
- Testicular diseases and treatments
- Cancer-related molecular mechanisms research
- Microfluidic and Bio-sensing Technologies
- Hypothalamic control of reproductive hormones
- Botanical Research and Applications
- Cancer, Stress, Anesthesia, and Immune Response
University of Florence
2015-2024
Azienda Ospedaliero-Universitaria Careggi
2023-2024
University of Siena
2014
Meyer Children's Hospital
2013
Sperm DNA fragmentation (sDF) represents a threat to male fertility, human reproduction and the health of offspring. The causes sDF are still unclear, even if apoptosis, oxidative assault defects in chromatin maturation hypothesized. Using multicolor flow cytometry sperm sorting, we challenged three hypothesized mechanisms by simultaneously evaluating signs damage (8-hydroxy, 2′-deoxyguanosine (8-OHdG) malondialdehyde (MDA)), apoptosis (caspase activity cleaved poly(ADP-ribose) polymerase...
Does CatSper have a role in the achievement of human sperm motility and Progesterone (P)-induced acrosome reaction (AR)? CatSper1 expression is associated with progressive P-induced AR; it may pathogenesis asthenozoospermia. Knockout mice for any Catsper family genes fail to acquire hyperactivated are infertile. channels mediate Ca2+ influx sperm. The hyperactivated/activated asthenospermia less clear. A few men mutations been described but phenotype regarding has not well established....
Predicting the outcome of in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) is one main goal present research on assisted reproduction. To understand whether density gradient centrifugation (DGC), used to select sperm, can affect DNA integrity and impact pregnancy rate (PR), we prospectively evaluated fragmentation (sDF) by TUNEL/PI, before after DGC. sDF was studied a cohort 90 infertile couples same day IVF/ICSI treatment. After DGC, increased 41 samples (Group A, median...
Sperm from many species share the sperm-specific Ca2+ channel CatSper that controls intracellular concentration and, thereby, swimming behaviour. A growing body of evidence suggests mechanisms controlling activity and its role during fertilization differ among species. lack suitable pharmacological tools has hampered elucidation function CatSper. Known inhibitors exhibit considerable side effects also inhibit Slo3, principal K+ mammalian sperm. The compound RU1968 was reported to suppress...
Sperm DNA fragmentation is a possible predictive parameter for male fertility status. The occurrence of M540 bodies in semen subfertile subjects affects flow cytometric investigations sperm. We set up new method to evaluate excluding bodies.DNA was evaluated by cytometry 75 both terminal deoxynucleotidyl transferase-mediated fluorescein-dUTP nick end labeling (TUNEL, traditional method) and double staining with TUNEL propidium iodide (PI, method).The use the revealed that underestimates...
The treatment of advanced prostate cancer (CaP) with androgen deprivation therapy inevitably renders the tumours castration resistant and incurable. Under these conditions, neuroendocrine differentiation (NED) CaP cells occurs neuropeptides released by facilitate tumour progression. Pharmacological strategies aiming to prevent or delay NED during ablation could, therefore, increase effectiveness therapy. Mechanisms pathways inducing in are poorly understood data often discordant. In present...
ABSTRACT: Techniques for assessing sperm DNA damage are numerous and various. There 2 main types of assay: direct indirect. The former directly detects the amount damage, whereas latter reveals effects an exogenous insult on chromatin. In addition, even considering same type technique, different strategies to reveal or quantify both, used. Finally, these techniques, except chromatin structure assay (SCSA), lack standardized protocols which all users can adhere minimize interlaboratory...
Oxidative stress (OS) is involved in many disoders including male infertility. Human spermatozoa are very sensitive targets of reactive oxygen species (ROS) and most sperm functions impaired the case OS. In addition unbalanced production ROS considered one important causes DNA fragmentation, a semen trait infertile men. The relationship between oxidative damage quality partially controversial, probably due to different methods and/or used reveal this study, by fluorescence microscopy flow...
STUDY QUESTIONIs CatSper1 expression in human spermatozoa related to semen parameter values and sperm functions?
Summary The aim of this study was to provide a comprehensive genetic/phenotypic characterization subjects suffering infertility owing sperm macrocephaly ( n = 3) or globozoospermia 9) and investigate whether the patients’ genetic status correlated with alteration various parameters. AURKC sequenced in case while DPY 19L2 has been analyzed by multiple approaches including novel qPCR –based copy number assay globozoospermia. Globozoospermic patients were also for SPACA 1 , candidate gene...
Our group has recently identified, in human semen, round bodies of different size and density which were termed M540 due to their staining with the fluorochrome merocyanine 540. Here, we investigate hypothesis that such structures represent apoptotic bodies. To this aim, by both fluorescence-activated cell sorting (FACS) fluorescence microscopy, examined occurrence markers as caspase activity, Fas, p53 Bcl-x In addition, evaluated ultrastructure transmission electron microscopy. We found...
Sumoylation is a post-translational modification involved in the regulation of several cell functions. Recent studies suggest its involvement spermatogenesis, but occurrence and function SUMO (small ubiquitin-like modifier) mature spermatozoa remain unknown. We report SUMO1-conjugated proteins, range 20–85 kDa, ejaculated spermatozoa. By cytofluorimetric analysis, we evaluated percentage SUMO1-positive 58 subjects undergoing semen analysis our laboratory correlated obtained values with...
What are the associations between semen apoptotic M540 bodies and other parameters of quality sonographic alterations male genital tract in a cohort infertile subjects? In subjects, M450 highly correlated with ultrasound clinical signs testis abnormalities but not parts tract, suggesting testicular origin bodies. We have reported presence round anucleate elements, named 'M540 bodies', resembling as they contain several markers. A consecutive series 130 males couple infertility were...
Epidemiological studies reported a negative relationship between concentrations of heavy metals and phthalates in seminal fluid semen quality, likely compromising male fertility potential. The aim this study was to investigate the vitro effects cadmium chloride (CdCl 2 ), common metal, diisobutyl phthalate (DIBP), ester, on human sperm functions necessary for fertilization. After incubation spermatozoa with 10 µM CdCl or 100 200 DIBP 24 h, significant decrease progressive hyperactivated...
Several clinical laboratories assess sperm DNA fragmentation (sDF) in addition to semen analysis male infertility diagnosis. Among tests evaluating sDF, TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) and SCD (Sperm Chromatin Dispersion) are widely used. Our lab developed a modified version of (TUNEL/PI) able distinguish two populations (PI Brighter PI Dimmer) differently associated with viability reproductive outcomes. The aim this study was compare sDF levels detected...