A5057232438- Milk Quality and Mastitis in Dairy Cows
- Probiotics and Fermented Foods
- Microbial Metabolism and Applications
- Animal Genetics and Reproduction
- RNA modifications and cancer
- Digestive system and related health
- Gut microbiota and health
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Infant Nutrition and Health
- RNA regulation and disease
- Mycobacterium research and diagnosis
Quebec Research and Development Centre
2022-2024
Agriculture and Agri-Food Canada
2022-2024
Government of Canada
2023
Business Development Bank of Canada
2023
International Trachoma Initiative
2022
Université de Montréal
2022
McGill University
2022
Sherbrooke Research and Development Centre
2022
Université Laval
2022
Abstract Background Mastitis caused by multiple factors remains one of the most common and costly disease dairy industry. Multi-omics approaches enable comprehensive investigation complex interactions between layers information to provide a more holistic view pathogenesis. Therefore, this study investigated genomic epigenomic signatures possible regulatory mechanisms underlying subclinical mastitis integrating RNA sequencing data (mRNA lncRNA), small (miRNA) DNA methylation milk somatic...
Staphylococcus aureus is one of the most prevalent contagious bacterial pathogen bovine mastitis. The subclinical mastitis it causes has long-term economic implications and difficult to control. To further understanding genetic basis mammary gland defense against S. infection, transcriptomes milk somatic cells from 15 cows with persistent natural infection (S. aureus-positive, SAP) 10 healthy control (HC) were studied by deep RNA-sequencing technology. Comparing SAP HC group revealed 4,077...
Staphylococcus chromogenes (SC) is a common coagulase-negative staphylococcus described as an emerging mastitis pathogen and commonly found in dairy farms. This study investigated the potential involvement of DNA methylation subclinical caused by SC. The whole-genome patterns transcriptome profiles milk somatic cells from four cows with naturally occurring SC (SCM) healthy were characterized next-generation sequencing, bioinformatics, integration analyses. Comparisons revealed abundant...
Abstract Staphylococcus aureus (S. aureus) is an opportunistic pathogen frequently associated with subclinical mastitis and accounts for a large proportion of the economic losses due to on Canadian dairy farms. Despite plethora investigations molecular mechanisms mastitis, little information available roles regulatory noncoding RNAs (ncRNA). This study aimed uncover microRNAs (miRNAs) long (lncRNAs) in response S. cows. Transcriptome sequencing (RNA-Seq miRNA-Seq) was conducted milk somatic...
<title>Abstract</title> Background Mastitis caused by multiple factors remains one of the most common and costly disease dairy industry. Multi-omics approaches enable comprehensive investigation complex interactions between layers information to provide a more holistic view pathogenesis. Therefore, this study investigated genomic epigenomic signatures possible regulatory mechanisms underlying subclinical mastitis integrating RNA sequencing data (mRNA lncRNA), small (miRNA) DNA methylation...
Abstract Staphylococcus chromogenes (SC), a frequent non-aureus staphylococcus on Canadian herds causes both clinical and subclinical forms of mastitis contributes to large economic losses farms. However, little research attention has been directed uncovering the molecular mechanisms underlying SC pathogenesis, including roles small nucleolar RNAs (snoRNAs). SnoRNAs function regulate host ribosomal (rRNAs) biogenesis processing, are known have regulatory functions in several human diseases,...
Abstract Background Mastitis caused by different pathogens including Streptococcus uberis ( S. ) is responsible for huge economic losses to the dairy industry. In order investigate potential genetic and epigenetic regulatory mechanisms of subclinical mastitis due , DNA methylome (whole genome methylation sequencing) transcriptome (RNA milk somatic cells from cows with naturally occurring healthy control (N = 3/group) were studied. Results Globally, levels CpG sites low in promoters first...
Abstract DNA methylation involvement in the regulation of mammary gland inflammatory defense against pathogens, including Staphylococcus aureus(SA) has been documented. However, mechanisms are not clear. The possible influence first introns (DNA-MeFI) on transcriptional activities during subclinical mastitis was studied by profiling methylome (whole genome sequencing) and transcriptome (RNA-sequencing) milk somatic cells from cows(n=15) with SA (SACs) healthy cows (HCs)(n=13), followed...
Abstract Staphylococcus aureus (S. aureus) is one of the most prevalent pathogens bovine subclinical mastitis and a challenging pathogen in dairy production. DNA methylation involvement regulating mammary gland inflammatory defense has been documented. This study adopted whole genome bisulfite sequencing RNA-sequencing technologies to profile genome-wide transcriptome landscapes, respectively, milk somatic cells (MSCs) from 15 cows with S. aureus-induced (SACs) 13 healthy (HCs)....