A5015479913- Connective Tissue Growth Factor Research
- Bone health and treatments
- Dermatological and Skeletal Disorders
- RNA Interference and Gene Delivery
- Cellular transport and secretion
- Lysosomal Storage Disorders Research
- Lipid Membrane Structure and Behavior
- Pancreatitis Pathology and Treatment
- Biomedical Research and Pathophysiology
- Advanced biosensing and bioanalysis techniques
- Studies on Chitinases and Chitosanases
- Protease and Inhibitor Mechanisms
- Mosquito-borne diseases and control
- Hormonal Regulation and Hypertension
- Peptidase Inhibition and Analysis
- Kruppel-like factors research
Molecular Discovery (United Kingdom)
2024
Max Planck Institute of Biochemistry
2020-2021
Universidad de Los Andes
2013-2015
Matrix metalloproteinases (MMPs) degrade several ECM components and are crucial modulators of cell invasion tissue organization. Although much has been reported about their function in remodeling health disease, trafficking across the Golgi apparatus remains poorly understood. Here we report that cis-Golgi protein nucleobindin-1 (NUCB1) is critical for MMP2 MT1-MMP along apparatus. This process Ca2+-dependent required invasive MDA-MB-231 migration as well gelatin degradation primary human...
<p>Supplementary Figure S1 shows the validation of ESTIMATE stromal and immune gene signatures mRNA expression in tumour-rich TME-rich samples.</p>
<p>Supplementary Figure S4 shows LPAR expression in PDAC cell lines and ATX-mediated pro-tumorigenic stimulation by 0082T conditioned media.</p>
<p>Supplementary Figure S5 shows RNA-seq analysis of 0082T CAFs after treatment with ATX inhibitors.</p>
<div>Abstract<p>Autotaxin (ATX), encoded by <i>ENPP2</i>, is a clinical target in pancreatic ductal adenocarcinoma (PDAC). ATX catalyzes the production of lysophosphatidic acid (LPA), an important regulator within tumor microenvironment (TME), yet protumorigenic action ATX/LPA axis PDAC remains unclear. In this study, interrogating patient samples and cell line datasets, we show that TME, rather than cancer cells, responsible for majority <i>ENPP2</i>...
<p>Supplementary Figure S2 shows the validation of 0082T CAF phenotype.</p>
<p>Supplementary Figure S6 shows LLPs expression in PDAC cancer and CAFs cells.</p>
<p>Supplementary Methods</p>
<p>Supplementary Figure S3 shows ATX antibody validation and detection in cancer CAF co-cultures.</p>
Abstract Autotaxin (ATX), encoded by ENPP2, is a clinical target in pancreatic ductal adenocarcinoma (PDAC). ATX catalyzes the production of lysophosphatidic acid (LPA), an important regulator within tumor microenvironment (TME), yet pro-tumorigenic action ATX/LPA axis PDAC remains unclear. Here, interrogating patient samples and cell line datasets, we show that TME, rather than cancer cells, responsible for majority ENPP2 expression, highlight key role associated fibroblast (CAF)-derived...
β-Galactosidase (BGal) is the first enzyme involved in catabolism of sphingolipids. Two pathologies have been directly associated with its deficiency: GM1 gangliosidosis and Morquio B. B among rarest types mucopolysaccharidosis (MPS). We aim to document β-galactosidase deficiency Colombia. evaluated leukocytes from 1492 healthy Colombian individuals 923 patients, referred between 2005 August 2014. Dried blood spot (DBS) samples same number patients were evaluated. was measured...
Monitoring vesicle trafficking is an excellent tool for the evaluation of protein dynamics in living cells. Such study key understanding sorting and secretion. Recent developments microscopy, as well new methodologies developed to synchronized proteins, allowed a better signaling, regulation at secretory pathway. One most helpful tools so far Retention Using Selective Hooks (RUSH) system, methodology that facilitates cargo by monitoring fluorescent vesicles cells upon biotin addition. Here...
More than 30% of the total amount proteins synthesized in mammalian cells follow secretory pathway order to mature and be properly sorted their final destinations. Among several methodologies that describe live-cell monitoring vesicles, Retention Using Selective Hooks (RUSH) system is a powerful one allows visualize cargo trafficking under physiological conditions. The present protocol describes method use RUSH microscopy subsequent quantitative analysis vesicles dissect protein trafficking....