A5052658115- Ion Transport and Channel Regulation
- Cutaneous Melanoma Detection and Management
- Birth, Development, and Health
- Melanoma and MAPK Pathways
- Pregnancy and preeclampsia studies
- vaccines and immunoinformatics approaches
- Gestational Diabetes Research and Management
- Pancreatic function and diabetes
- Advanced Proteomics Techniques and Applications
- Ion channel regulation and function
- ATP Synthase and ATPases Research
- Actinomycetales infections and treatment
- Mass Spectrometry Techniques and Applications
- Mitochondrial Function and Pathology
- Sulfur Compounds in Biology
- Metalloenzymes and iron-sulfur proteins
- Molecular Biology Techniques and Applications
- Contact Dermatitis and Allergies
- Nonmelanoma Skin Cancer Studies
- Chronic Kidney Disease and Diabetes
- Digital Imaging in Medicine
- Pancreatic and Hepatic Oncology Research
- Mycobacterium research and diagnosis
- Hydrogen Storage and Materials
- AI in cancer detection
The University of Sydney
2016-2025
Westmead Institute for Medical Research
2021-2025
Westmead Hospital
2021-2025
Westmead Institute
2025
Royal North Shore Hospital
2017
Sir Charles Gairdner Hospital
2013
Multiple rodent models have been used to study diabetic kidney disease (DKD). The purpose of the present was compare diabetes and obesity-induced metabolic syndrome determine differences in renal outcomes. C57BL/6 male mice were fed either normal chow or high fat diet (HFD). At postnatal week 8, chow-fed randomly assigned low-dose streptozotocin (STZ, 55 mg/kg/day, five consecutive days) vehicle control, whereas HFD-fed given one high-dose STZ (100 mg/kg) control. Intraperitoneal glucose...
Aims/Hypothesis Developmental programming induced by maternal obesity influences the development of chronic disease in offspring. In present study, we aimed to determine whether exaggerates obesity-related kidney disease. Methods Female C57BL/6 mice were fed high-fat diet (HFD) for six weeks prior mating, during gestation and lactation. Male offspring weaned normal chow or HFD. At postnatal Week 8, HFD-fed administered one dose streptozotocin (STZ, 100 mg/kg i.p.) vehicle control. Metabolic...
Abstract Maternal obesity is known to increase the risk of and diabetes in offspring. Though a key factor for development chronic kidney disease (CKD), relationship between maternal CKD has not been clearly defined. In this study, mouse model was employed determine impact on diabetic nephropathy Female C57BL/6 mice were fed high-fat diet (HFD) six weeks prior mating, during gestation lactation. Male offspring weaned normal chow diet. At postnatal Week 8, randomly administered low dose...
FXYD3 is a Na/K-ATPase modulator which upregulated in pancreatic ductal adenocarcinoma (PDAC), but its prognostic role unknown. This study evaluated expression chemo-naive patients with surgically-resected PDAC at single centre (1993-2014). was assessed tumour specimens using immunohistochemistry. 145 of 180 were FXYD3-immunopositive (80.5%). There no difference median overall survival between the negative (27.60 months) and positive groups (25.00 (log-rank p = 0.9718). correlated positively...
Despite recent developments in managing metastatic melanomas, patients' overall survival remains low. Therefore, the current study aims to understand better proteome-wide changes associated with melanoma metastasis that will assist identifying targeted therapies. The latest development mass spectrometry-based proteomics, together extensive bioinformatics analysis, was used investigate molecular 60 formalin-fixed and paraffin-embedded samples of primary lymph nodes (LN) distant organ...
The seven mammalian FXYD proteins associate closely with α/β heterodimers of Na+/K+-ATPase. Most them protect the β1 subunit against glutathionylation, an oxidative modification that destabilizes heterodimer and inhibits Na+/K+-ATPase activity. A specific cysteine (Cys) residue is critical for such protection. One proteins, FXYD3, confers treatment resistance when overexpressed in cancer cells. We developed two FXYD3 peptide derivatives. FXYD3-pep CKCK retained Cys can undergo...
The collection of human stratum corneum (SC) samples by tape-stripping promises to deliver a non-invasive method for biomarker analysis skin diseases. current study examines the effect storage temperature and SC layer depth on proteome profile samples.
Abstract Background Only a small per cent of new melanocytic lesions developing in adults are expected to represent melanomas. Total body photography (TBP) has been widely incorporated clinical practice, especially for follow‐up high‐risk individuals with multiple naevi. However, dynamic changes detected TBP need be interpreted caution avoid unnecessary excisions. Objectives To identify and dermoscopic predictors malignancy presenting clinically as on TBP. Methods Melanomas naevi excised...
The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via PRIDE [1] partner repository with dataset identifier PXD025791. Please note: publisher is not responsible for content or functionality of any supporting information supplied by authors. Any queries (other than missing content) should be directed corresponding author article.
Rationale: Cardiotonic steroids (CTS), such as digoxin, have been used to treat heart failure (HF) for over 200 years. They inhibit the sodium-potassium pump (NKP), and increase cardiac contractility by inhibiting efflux of sodium through (“digitalis hypothesis”). CTS possess three structural components: a saturated/unsaturated lactone ring, steroid core, sugar moiety, each which may be involved in NKP inhibition/stimulation. It is now known that inhibition patients with HF increases...
Abstract Background FXYD proteins associate closely with- and protect plasmalemmal Na + /K -ATPase against oxidative inhibition. One of them, FXYD3, is often overexpressed in cancers, including those breast pancreas. Down-regulation overexpression MCF-7 cancer cells with siRNA augments doxorubicin-induced cytotoxicity. Because down-regulation not readily translated therapeutically, we developed a peptide as an alternative for suppression FXYD3. Methods A shortened derivative the wild-type...