A5101905457- Trypanosoma species research and implications
- Calcium signaling and nucleotide metabolism
- Sirtuins and Resveratrol in Medicine
- Research on Leishmaniasis Studies
- Coenzyme Q10 studies and effects
- Cardiomyopathy and Myosin Studies
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Hepatocellular Carcinoma Treatment and Prognosis
- Adenosine and Purinergic Signaling
- Mitochondrial Function and Pathology
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- Ectopic Pregnancy Diagnosis and Management
- Liver Disease Diagnosis and Treatment
- Erythrocyte Function and Pathophysiology
- Synthesis and Biological Evaluation
- Gestational Trophoblastic Disease Studies
- Biochemical effects in animals
- Synthesis of Organic Compounds
- Apelin-related biomedical research
- Redox biology and oxidative stress
- Biomedical Research and Pathophysiology
- Blood groups and transfusion
- Genomics, phytochemicals, and oxidative stress
- Viral Infections and Immunology Research
The University of Texas Medical Branch at Galveston
2012-2021
Eastern Hepatobiliary Surgery Hospital
2014-2018
Second Military Medical University
2014-2018
Galveston College
2016
Women's Hospital, School of Medicine, Zhejiang University
2006
Chronic chagasic cardiomyopathy (CCM) is presented by increased oxidative/inflammatory stress and decreased mitochondrial bioenergetics. SIRT1 senses the redox changes integrates metabolism inflammation; deficiency may be a major determinant in CCM. To test this, C57BL/6 mice were infected with Trypanosoma cruzi (Tc), treated agonists (resveratrol or SRT1720), monitored during chronic phase (~150 days post-infection). Resveratrol treatment was partially beneficial controlling pathologic...
Metabolism provides substrates for reactive oxygen species (ROS) and nitric oxide (NO) generation, which are a part of the macrophage (mφ) anti-microbial response. Mφs infected with Trypanosoma cruzi (Tc) produce insufficient levels oxidative lower glycolysis compared to classically-activated mφs. How mφs fail elicit potent ROS/NO response during infection its link is unknown. Herein, we evaluated ROS, NO, cytokine production in presence metabolic modulators Krebs cycle. Metabolic status was...
Trypanosoma cruzi is the causative agent of chronic chagasic cardiomyopathy. Why macrophages (mφs), early responders to infection, fail achieve parasite clearance not known. Mouse (RAW 264.7) and human (THP-1 primary) mφs were infected for 3 h 18 with T. TcI isolates, SylvioX10/4 (SYL, virulent) TCC (nonpathogenic), which represent mφ stimulation infection states, respectively. Mφs incubated lipopolysaccharide gamma interferon (LPS/IFN-γ) interleukin-4 (IL-4) used as controls. We monitored...
Background Mitochondrial dysfunction is a key determinant in chagasic cardiomyopathy development mice; however, its relevance human C hagas disease not known. We determined if defects mitochondrial biogenesis and dysregulation of peroxisome proliferator‐activated receptor gamma ( PPAR γ) coactivator‐1 PGC ‐1)–regulated transcriptional pathways constitute mechanism or mechanisms underlying oxidative‐phosphorylation OXPHOS ) deficiency disease. Methods Results utilized cardiomyocytes...
Background We utilized genetically modified mice equipped with a variable capacity to scavenge mitochondrial and cellular reactive oxygen species investigate the pathological significance of oxidative stress in C hagas disease. Methods Results C57 BL /6 (wild type, Mn SOD tg , +/− GP x1 −/− ) were infected Trypanosoma cruzi harvested during chronic disease phase. Chronically exhibited substantial increase plasma levels inflammatory markers (nitric oxide, myeloperoxidase), lactate...
Recently immunoglobulin G (IgG) was found to be produced by neoplasms and promote tumor growth in cancer cell lines animal models. To investigate the pathophysiological significance of cancerproduced IgG breast cancer, we examined expressions 68 cancers including 40 primary without metastasis 28 with axillary lymph node metastases. gene expression detected all these samples. We that IgG-expressing cells were predominantly located periphery nest showed more cellular atypia nuclear...
Trypanosoma cruzi (Tc) causes Chagas disease (CD) that is the most frequent cause of heart failure in Latin America. TNF-α+ monocytes/macrophages (Mo/Mφ) are associated with inflammatory pathology chronic CD. In this study, we determined progenitor lineage Mo/Mφ contributing to inflammation and examined regulatory role SIRT1 modulating response disease.C57BL/6 mice were infected Tc, treated agonist (SRT1720) after control acute parasitemia, monitored during phase (150 days post-infection)....
Molecular mechanisms of Trypanosoma cruzi (Tc)-induced Chagasic cardiomyopathy (CCM) are not well understood. The NO-cGMP-PKG1α pathway maintains cardiac homeostasis and inotropy may be disturbed due to phosphodiesterase (PDE5)-mediated cGMP catabolism in CCM. To test this, C57BL/6 mice were infected with T. cruzi, after the control acute parasitemia (∼45 days post-infection), given sildenafil (SIL) (1 mg/kg) treatment for 3 weeks that ended long before chronic disease phase (∼150...
Background Chagas disease, caused by Trypanosoma cruzi, is endemic in Latin America and an emerging infectious disease the US Europe. We have shown TcG1, TcG2, TcG4 antigens elicit protective immunity to T. cruzi mice dogs. Herein, we investigated antigenicity of recombinant proteins humans determine their potential utility for development next generation diagnostics screening infection disease. Methods Results Sera samples from inhabitants areas Argentina-Bolivia Mexico-Guatemala were...
The mechanism of aberrant mitochondrial genome and function in hepatocellular carcinoma (HCC) remains largely unknown. Our previous study demonstrated an increased expression aspartate β-hydroxylase (ASPH) HCC tissues, which was associated with tumor invasiveness a worse prognosis. Currently, we unexpectedly observed the presence ASPH purified protein fraction. In addition, immunostaining both exogenously endogenously expressed showed colocalization biomarkers. This aimed to investigate...
Extrauterine choriocarcinoma is a rare form of gestational trophoblastic tumor. the greater omentum extremely in literature. A 24-year-old female with irregular vaginal bleeding, mimicking as ectopic pregnancy, underwent emergency exploratory laparotomy and local excision mass. The serum beta-human chorionic gonadotropin level decreased rapidly after operation; however, it rose up again before long. Pathology showed omentum. No evidence lesions on uterus. other metastasis was found. Multiple...
Chronic chagasic cardiomyopathy (CCM) is presented with ventricular hypertrophy and contractile dysfunction that can lead to heart failure. I have found a substantial decline in mitochondrial biogenesis SIRT1/PGC-1α activity ensue chronic mice. It was evidenced by the DNA content as well mRNA levels of encoded genes mtDNA replication machinery. Further, SIRT1 (required for PGC-1α activation) decreased associated nuclear PGC-1-regulated NRF1 transcription factor hearts. The size number were...