A5065438894- Pain Mechanisms and Treatments
- Ion Channels and Receptors
- Neurobiology and Insect Physiology Research
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Herbal Medicine Research Studies
- Nicotinic Acetylcholine Receptors Study
- Ion channel regulation and function
- Exercise and Physiological Responses
Max Planck Institute of Experimental Medicine
2015-2016
Nephrologisches Zentrum Goettingen
2015
Max Planck Society
2014
Chronic pain is a complex disease with limited treatment options. Several profiling efforts have been employed the aim to dissect its molecular underpinnings. However, generated results are often inconsistent and nonoverlapping, which largely because of inherent technical constraints. Emerging data-independent acquisition (DIA)-mass spectrometry (MS) has potential provide unbiased, reproducible quantitative proteome maps - prerequisite for standardization among experiments. Here, we designed...
The transient receptor potential A1 (TRPA1) channel is essential for vertebrate pain. Even though TRPA1 activation by ligands has been studied extensively, the molecular machinery regulating only poorly understood. Using an unbiased proteomics-based approach we uncovered physical association of Annexin A2 (AnxA2) with native in mouse sensory neurons. AnxA2 enriched a subpopulation neurons and coexpressed TRPA1. Furthermore, observe increase membrane levels cultured from AnxA2-deficient mice....
The ability of sensory neurons to detect potentially harmful stimuli relies on specialized molecular signal detectors such as transient receptor potential (TRP) A1 ion channels. TRPA1 is critically implicated in vertebrate nociception and different pain states. Furthermore, channels are subject extensive modulation regulation - processes which consequently affect nociceptive signaling. Here we show that the neuropeptide Nocistatin sensitizes TRPA1-dependent calcium influx upon application...