A5073770061- Genetics, Aging, and Longevity in Model Organisms
- Circadian rhythm and melatonin
- Advanced Electron Microscopy Techniques and Applications
- Neurogenesis and neuroplasticity mechanisms
- Proteins in Food Systems
- Advanced Fluorescence Microscopy Techniques
- Neuroendocrine regulation and behavior
- Molecular Biology Techniques and Applications
- Anodic Oxide Films and Nanostructures
- Neuroinflammation and Neurodegeneration Mechanisms
- Regulation of Appetite and Obesity
- Neurobiology and Insect Physiology Research
- Photosynthetic Processes and Mechanisms
- Planarian Biology and Electrostimulation
- Autophagy in Disease and Therapy
- Invertebrate Immune Response Mechanisms
- Plant Molecular Biology Research
- Smart Materials for Construction
- Microtubule and mitosis dynamics
- Wnt/β-catenin signaling in development and cancer
- Hedgehog Signaling Pathway Studies
- Ferroptosis and cancer prognosis
- Protein Hydrolysis and Bioactive Peptides
- Microencapsulation and Drying Processes
- Photoreceptor and optogenetics research
University of Lausanne
2018-2025
Université de Rennes
2012-2019
Structure Fédérative de Recherche en Biologie et Santé de Rennes
2015-2018
Centre National de la Recherche Scientifique
2009-2018
Imaging Center
2018
Institut de génétique et de développement de Rennes
2014-2018
Inserm
2009-2015
La Ligue Contre le Cancer
2015
Université de Strasbourg
2013
Institut de génétique et de biologie moléculaire et cellulaire
2009-2013
The interplay between signalling pathways and metabolism is crucial for tissue growth. Yet, it remains poorly understood. Here, we studied the consequences of modulating iron on growth Drosophila imaginal discs. We find that reducing levels ferritin heavy chain in larval wing discs leads to drastic defects, whereas light depletion causes only minor defects. Mutant cell clones lack ability compete against Minute mutant cells. Reactive oxygen species (ROS) accumulate with reduced levels,...
Using electron microscopy to localize rare cellular events or structures in complex tissue is challenging. Correlative light and procedures have been developed link fluorescent protein expression with ultrastructural resolution. Here, we present an optimized scanning (SEM) workflow for volumetric Array Tomography asymmetric samples model organisms (Caenorhabditis elegans, Drosophila melanogaster, Danio rerio). We modified a diamond knife simplify serial section arrays acquisition minimal...
CLEM (correlative live cell and electron microscopy) seeks to bridge the data acquired with different imaging strategies, typically between light microscopy microscopy. It has been successfully applied in cultures, although its use multicellular systems is hampered by difficulties locating ROI (region of interest).We developed a technique that enables easy processing small model animals adequate both for morphology immunoelectron-microscopic specimen preparations. While this method initially...
ABSTRACT To sense the outside world, some neurons protrude across epithelia, cellular barriers that line every surface of our bodies. study morphogenesis such neurons, we examined C. elegans amphid, in which dendrites through a glial channel at nose. During development, amphid extend by attaching to nose via DYF-7, type protein typically found epithelial apical ECM. Here, show and glia exhibit properties, including tight junctions apical-basal polarity, develop manner resembling other...
Tanycytes are highly specialized ependymal cells that line the bottom and lateral walls of third ventricle. In contact with cerebrospinal fluid through their cell bodies, they send processes into arcuate nucleus, ventromedial dorsomedial nucleus hypothalamus. present work, we combined transgenic immunohistochemical approaches to investigate neuroanatomical associations between tanycytes neural in hypothalamic parenchyma, particular nucleus. The specific expression tdTomato first allowed...
The small ubiquitin-like modifier (SUMO) modification alters the subcellular distribution and function of its substrates. Here we show major role SUMO during development Caenorhabditis elegans reproductive system. smo-1 deletion mutants develop into sterile adults with abnormal somatic gonad, germ line, vulva. SMO-1::GFP reporter is highly expressed in animals lack a vulval-uterine connection as result impaired ventral uterine π-cell differentiation anchor cell fusion. Mutations LIN-11 LIM...
Apical extracellular matrix (aECM) constitutes the interface between every tissue and outside world. It is patterned into diverse tissue-specific structures through unknown mechanisms. Here, we show that a male-specific genetic switch in single C. elegans glial cell patterns overlying aECM from solid sheet to an ∼200 nm pore, thus allowing male sensory neuron access environment. Using cell-specific sex reversal, find this reflects inherent difference independent of identity surrounding...
ABSTRACT Intercellular bridges are essential to connect developing germline cells. The Drosophila melanogaster egg chamber is a powerful model system study intercellular bridges, or ring canals (RCs). RCs the oocyte supporting nurse cells, and defects in their stability growth lead infertility. Despite importance, it has been technically difficult use electron microscopy-based approaches monitor changes RC structure during oogenesis. Here, we describe application of complementary set volume...
Abstract Transmission electron microscopy (TEM) is an important tool for studies in cell biology, and essential to address research questions from bacteria animals. Recent technological innovations have advanced the entire field of TEM, yet classical techniques still prevail most present-day studies. Indeed, majority developmental biology that use TEM do not require cutting-edge methodologies, but rather fast efficient data generation. Although access state-of-the-art equipment frequently...
Significance This work addresses the interplay among membrane trafficking, cell adhesion, and tissue integrity maintenance in Drosophila female germline. The clathrin adaptor protein 1 (AP-1) complex is shown to regulate trafficking of E-cadherin ring canals (RCs), a structure resulting from incomplete cytokinesis allowing intercellular communication. assembles adhesive clusters that, as revealed by EM analyses, organize dense microvilli meshwork wrapping around RCs. Although dispensable for...
Glucokinase (GCK) is critical for glucosensing. In rats, GCK expressed in hypothalamic tanycytes and appears to play an essential role feeding behavior. this study, we investigated the distribution of GCK-expressing mice their regulation energy balance. situ hybridization, reporter gene assay, immunohistochemistry were used assess expression along third ventricle mice. To evaluate impact on arcuate neuron function mouse physiology, Gck deletion was achieved using loxP/Cre recombinase...
Here, we describe a new muscle LIM domain protein, UNC-95, and identify it as novel target for the RING finger protein RNF-5 in Caenorhabditis elegans body wall muscle. unc-95(su33) animals have disorganized actin myosin-containing filaments result of failure to assemble normal adhesion structures. UNC-95 is active downstream PAT-3/β-integrin assembly pathways dense M-line attachments, upstream DEB-1/vinculin pathway. The translational UNC-95::GFP fusion construct expressed bodies, M-lines,...
Throughout metazoans, germ cells undergo incomplete cytokinesis to form syncytia connected by intercellular bridges. Gamete formation ultimately requires bridge closure, yet how bridges are reactivated close is not known. The most conserved component centralspindlin, a complex of the Rho family GTPase-activating protein (GAP) CYK-4/MgcRacGAP and microtubule motor ZEN-4/kinesin-6. Here, we show that oocyte production syncytial Caenorhabditis elegans germline CYK-4 but ZEN-4, which contrasts...
Abstract To compare vulva development mechanisms in the nematode Oscheius sp. 1 to those known Caenorhabditis elegans, we performed a genetic screen for mutants CEW1. Here present one large category of mutations that call cov, which affect specification Pn.p ventral epidermal cells along antero-posterior axis. The are numbered from 12 anterior posterior. In wild-type CEW1, P(4-8).p competent form and progeny P(5-7).p actually vulva, with descendants P6.p adopting central vulval fate. Among...
(Macro)autophagy is a major lysosome-dependent degradation mechanism which engulfs, removes and recycles unwanted cytoplasmic material, including damaged organelles toxic protein aggregates. Although few studies implicate autophagy in CNS demyelinating pathologies, its role, particularly mature oligodendrocytes myelin, remains poorly studied. Here, using both pharmacological genetic inhibition of the autophagic machinery, we provide evidence that an essential for oligodendrocyte maturation...