A5074941305- Medical Imaging and Pathology Studies
- Trypanosoma species research and implications
- Parvovirus B19 Infection Studies
- Sarcoma Diagnosis and Treatment
- MRI in cancer diagnosis
- Insect symbiosis and bacterial influences
- Biochemical and Molecular Research
- T-cell and Retrovirus Studies
- Ubiquitin and proteasome pathways
- Parasitic Diseases Research and Treatment
- Studies on Chitinases and Chitosanases
- Immune Cell Function and Interaction
- Glycosylation and Glycoproteins Research
- CRISPR and Genetic Engineering
- Cancer-related Molecular Pathways
- Cytomegalovirus and herpesvirus research
- Research on Leishmaniasis Studies
- Religion, Society, and Development
- Cardiomyopathy and Myosin Studies
- Autophagy in Disease and Therapy
University of Oxford
2018-2025
MRC Protein Phosphorylation and Ubiquitylation Unit
2018
University of Dundee
2018
Google (United States)
2017
Australian Institute of Health and Welfare
2001
Clustered regularly interspaced short palindromic repeats (CRISPR), CRISPR-associated gene 9 (Cas9) genome editing is set to revolutionize genetic manipulation of pathogens, including kinetoplastids. CRISPR technology provides the opportunity develop scalable methods for high-throughput production mutant phenotypes. Here, we report development a CRISPR-Cas9 toolkit that allows rapid tagging and knockout in diverse kinetoplastid species without requiring user perform any DNA cloning. We...
Deubiquitinating enzymes (DUBs) are important regulators of ubiquitin signaling. Here, we report the discovery deubiquitinating activity in ZUFSP/C6orf113. High-resolution crystal structures ZUFSP complex with reveal several distinctive features recognition and catalysis. Our analyses that is a novel DUB no homology to any known DUBs, leading us classify as seventh family. Intriguingly, minimal catalytic domain does not cleave polyubiquitin. We identify two binding domains ZUFSP: ZHA (ZUFSP...
Trypanosoma brucei is a model trypanosomatid, an important group of human, animal and plant unicellular parasites. Understanding their complex cell architecture life cycle challenging because, as with most eukaryotic microbes, ~50% genome-encoded proteins have completely unknown functions. Here, using fluorescence microscopy lines expressing endogenously tagged proteins, we mapped the subcellular localization 89% T. proteome, resource call TrypTag. We provide clues to function define...
The kinetoplastids Trypanosoma brucei and Leishmania mexicana are eukaryotes with a highly structured cellular organisation that is reproduced great fidelity in each generation. pattern of signal from fluorescently tagged protein can define the specific structure/organelle this localises to, be extremely informative phenotype analysis experimental perturbations, life cycle tracking, post-genomic assays functional organelles. Using vast coverage subcellular localisations provided by TrypTag...
Abstract Trypanosoma brucei is a prototypical trypanosomatid, an important group of human, animal and plant unicellular parasites. Understanding their complex cell architecture life cycle hindered since, as with most eukaryotic microbes, ∼50% the proteins encoded in genome have completely unknown function. Using fluorescence microscopy lines expressing endogenously tagged we mapped subcellular localisation 89% proteome, giving clues to function, defining lineage-specific organelle...