A5038771747- Milk Quality and Mastitis in Dairy Cows
- Probiotics and Fermented Foods
- Infant Nutrition and Health
- Gene expression and cancer classification
- Bioinformatics and Genomic Networks
- Fibroblast Growth Factor Research
- Veterinary Oncology Research
- Rheumatoid Arthritis Research and Therapies
- Hormonal and reproductive studies
- Advanced Proteomics Techniques and Applications
- TGF-β signaling in diseases
- NF-κB Signaling Pathways
- Biopolymer Synthesis and Applications
- Chromosomal and Genetic Variations
- Treatment of Major Depression
- Glycosylation and Glycoproteins Research
- Birth, Development, and Health
- Research on Leishmaniasis Studies
- Gene Regulatory Network Analysis
- Prostate Cancer Treatment and Research
- Skin and Cellular Biology Research
- Machine Learning in Bioinformatics
- Medical Imaging and Analysis
- Cancer, Hypoxia, and Metabolism
- Cancer, Lipids, and Metabolism
University of Glasgow
2012-2020
Glasgow Life
2016
Département Santé Animale
2016
University of Dundee
2011-2014
B.J. Medical College
2014
The polarization of macrophages into a regulatory-like phenotype and the production IL-10 plays an important role in resolution inflammation. We show this study that PGE2, combination with LPS, is able to promote anti-inflammatory characterized by high expression regulatory markers SPHK1 LIGHT via protein kinase A–dependent pathway. Both TLR agonists PGE2 phosphorylation transcription factor CREB on Ser133. However, although regulates transcription, mutation Ser133 Ala endogenous gene did...
We present the first comparison of global transcriptional changes in canine and human diffuse large B-cell lymphoma (DLBCL), with particular reference to nuclear factor-kappa B (NF-κB) pathway. Microarray data generated from DLBCL normal lymph nodes were used for differential expression, co-expression pathway analyses, compared analysis microarray healthy nodes. The comparisons at gene level performed by mapping probesets microarrays orthologous genes humans vice versa. A considerable number...
Proteomics-based approaches for biomarker discovery are promising strategies used in cancer research. We present state-of-art label-free quantitative proteomics method to assess proteome of renal cell carcinoma (RCC) compared with noncancer tissues. Fresh frozen tissue samples from eight primary RCC lesions and autologous adjacent normal tissues were obtained surgically resected tumour-bearing kidneys. Proteins extracted by complete solubilisation using filter-aided sample preparation (FASP)...
The differentiation of mouse embryonic stem cells (ESCs) is controlled by the interaction multiple signaling pathways, typically mediated post-translational protein modifications. addition O-linked N-acetylglucosamine (O-GlcNAc) to serine and threonine residues nuclear cytoplasmic proteins one such modification (O-GlcNAcylation), whose function in ESCs only now beginning be elucidated. Here, we demonstrate that specific inhibition O-GlcNAc hydrolase (Oga) causes increased levels...
Remyelination failure plays an important role in the pathophysiology of multiple sclerosis, but underlying cellular and molecular mechanisms remain poorly understood. We now report actively demyelinating lesions patients with sclerosis are associated increased glial expression fibroblast growth factor 9 (FGF9), which we demonstrate inhibits myelination remyelination vitro. This inhibitory activity is appearance multi-branched 'pre-myelinating' MBP+/PLP+ oligodendrocytes that interact axons...
Amphotericin B has emerged as the therapy of choice for use against leishmaniases. Administration drug in its liposomal formulation a single injection is being promoted campaign to bring leishmaniases under control. Understanding risks and mechanisms resistance therefore great importance. Here we select amphotericin B-resistant Leishmania mexicana parasites with relative ease. Metabolomic analysis demonstrated that ergosterol, sterol known bind drug, prevalent wild-type cells, but diminished...
Longitudinal proteomic analysis of bovine milk shows consistent changes over time across cows after intramammary challenge with<italic>Streptococcus uberis</italic>.
A peptidomic investigation of milk from an experimental model Streptococcus uberis mastitis in dairy cows has incorporated a study high abundance and acute phase (APP) proteins as well analysis low molecular weight peptide biomarkers. Intramammary infection (IMI) with S. caused shift caseins, β-lactoglobulin α-lactalbumin to albumin, lactoferrin IgG the increase occurring last. The APP response haptoglobin, mammary associated serum amyloid A3 C-reactive protein occurred between 30-48 hours...
Metabolomic investigation of milk from cows with bovine mastitis has revealed major changes in carbohydrates, lipids, amino acids, nucleotides and bile acids.
Changes in the plasma metabolic profile were characterised newly diagnosed rheumatoid arthritis (RA) patients upon commencement of conventional disease-modifying anti-rheumatic drug (cDMARD) therapy. Plasma samples collected an early RA randomised strategy study (NCT00920478) that compared clinical (DAS) disease activity assessment with musculoskeletal ultrasound (MSUS) to drive treatment decisions subjected untargeted metabolomic analysis. Metabolic profiles at pre- and three months...
Non-negative matrix factorization is a useful tool for reducing the dimension of large datasets. This work considers simultaneous non-negative multiple sources data. In particular, we perform first study that involves more than two We discuss algorithmic issues required to convert approach into practical computational and apply technique new gene expression data quantifying molecular changes in four tissue types due different dosages an experimental panPPAR agonist mouse. interest toxicology...
Dietary restriction (DR) is the most widely studied non-genetic intervention capable of extending lifespan across multiple taxa.Modulation genes, primarily within insulin/insulin-like growth factor signalling (IIS) and mechanistic target rapamycin (mTOR) pathways also act to extend in model organisms.For example, mice lacking insulin receptor substrate-1 (IRS1) are long-lived protected against several age-associated pathologies.However, it remains unclear how these particular interventions...
Biomarker identification, using network methods, depends on finding regular co-expression patterns; the overall connectivity is of greater importance than any single relationship. A second requirement a simple algorithm for ranking patients how relevant gene-set is. For both these requirements discretized data helps to first identify gene cliques, and then stratify patients. We explore biologically intuitive discretization technique which codes genes as up- or down-regulated, with values...
Background It is unclear whether a short-term change in circulating androgens associated with changes the transcriptome of peripheral blood mononuclear cells (PBMC). Aims and methods To explore effect hCG stimulation on PBMC transcriptome, 12 boys median age (range) 0.7 years (0.3, 11.2) who received intramuscular 1500u 3 consecutive days as part their investigations underwent transcriptomic array analysis RNA extracted from before after stimulation. Results Median pre- post-hCG testosterone...
Target selection is crucial in pharmaceutical drug discovery, directly influencing clinical trial success. Despite its importance, development remains resource-intensive, often taking over a decade with significant financial costs. High failure rates highlight the need for better early-stage target selection. We present GATher, graph attention network designed to predict therapeutic gene-disease links by integrating data from diverse biomedical sources into 4.4 million edges. GATher...
Abbreviated new drug application is required to submit United States Food and Drug Administration for approval of the generic version an innovator drug. This (DuLocap) ® duloxetine hydrochloride delayed release (DR) capsules 60 mg manufactured by alpha pharmaceuticals. abridged claiming essential similarity brand product that is, Cymbalta (duloxetine HCL DR capsules) mg, mfg Lily S.A, Spain marketed Eli Lilly, filed on basis para III (patent expires June 11, 2013).
Abstract Objective To characterize changes in the plasma metabolic profile newly diagnosed rheumatoid arthritis (RA) patients upon commencement of conventional disease modifying anti-rheumatic drug (cDMARD) therapy. Methods Plasma samples collected an early RA randomized strategy study ( NCT00920478 ) that compared clinical (DAS) activity assessment with musculoskeletal ultrasound (MSUS) to drive treatment decisions were subjected untargeted metabolomic analysis. Metabolic profiles at pre-...