A5035157068- Reproductive Biology and Fertility
- Genetic Mapping and Diversity in Plants and Animals
- Animal Genetics and Reproduction
- Meat and Animal Product Quality
- Genetic and phenotypic traits in livestock
- Ruminant Nutrition and Digestive Physiology
- Reproductive Physiology in Livestock
- MicroRNA in disease regulation
- Molecular Biology Techniques and Applications
- Sperm and Testicular Function
- Animal Behavior and Welfare Studies
- Microbial infections and disease research
- Animal Virus Infections Studies
- Agroforestry and silvopastoral systems
- Cancer-related molecular mechanisms research
- Agriculture Sustainability and Environmental Impact
- Pluripotent Stem Cells Research
- Sustainable Agricultural Systems Analysis
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Animal Disease Management and Epidemiology
- TGF-β signaling in diseases
- Medicinal Plant Pharmacodynamics Research
- Muscle Physiology and Disorders
- Plant and fungal interactions
- Pasture and Agricultural Systems
Chiang Mai University
2010-2023
University of Bonn
2007-2014
Institute of Animal Science
2009
Research Institute for Farm Animal Biology (FBN)
2008
The accumulation of maternal mRNA and protein during oogenesis for supporting oocyte maturation the newly fertilised zygote marks beginning developmental process in mammals. MicroRNAs (approximately 18-22 nt long) which are known post-transcriptional gene regulation evidenced their essential role animal development. We, therefore, aimed to investigate expression miRNAs immature vitro matured bovine oocytes, using heterologous miRNA array platform. To attain this, we used a mercury locked...
MicroRNAs are the major class of gene-regulating molecules playing diverse roles through sequence complementarity to target mRNAs at post-transcriptional level. Tightly regulated expression and interaction a multitude genes for ovarian folliculogenesis could be by these miRNAs. Identification them is first step towards understanding miRNA-guided gene regulation in different biological functions. Despite increasing efforts miRNAs identification across various species tissue types, little...
Oocyte selection based on glucose-6-phosphate dehydrogenase (G6PDH) activity has been successfully used to differentiate between competent and incompetent bovine oocytes. However, the intrinsic molecular subcellular characteristics of these oocytes have not yet investigated. Here, we aim identify functional markers associated with oocyte developmental potential when selected G6PDH activity. Immature compact cumulus–oocyte complexes were stained brilliant cresyl blue (BCB) for 90 min. Based...
This study was conducted to investigate the gene expression profile of in vivo -derived bovine embryo biopsies based on pregnancy outcomes after transferring recipients. For this, 30–40% embryos were taken from grade I blastocysts (International Embryo Transfer Society Manual) and remaining 60–70% intact transferred Frozen pooled into three distinct groups outcome corresponding parts, namely those resulting no (NP), loss (PL), calf delivery (CD). Array analysis revealed a total 41 43 genes...
Abstract Background Leakage of water and ions soluble proteins from muscle cells occurs during prolonged exercise due to ischemia causing damage. Also post mortem anoxia conversion meat is marked by loss components the cell. There considerable variation in holding capacity affecting economy production. Water depends on numerous genetic environmental factors relevant structural biochemical fibre properties a well as ante slaughter metabolic processes. Results Expression microarray analysis M....
The Well of the (WOW) system has been developed to culture embryos in small groups or track development single embryos. In present study, we aimed examine effects microenvironment provided by WOW and embryo density on developmental rates, quality preimplantative gene expression profile resulting Embryos cultured a group 16 reached blastocyst stage at significantly lower level than zygotes 50 (22.2 vs 30.3%), whereas were able compensate against low densities, reaching rate as high (31.3...
To identify biological processes as well molecular markers for drip loss, a parameter water holding capacity of meat, the M. longissimus dorsi transcriptomes six divergent sib pairs were analyzed using Affymetrix Porcine Genome Array. Functional categories differentially regulated transcripts determined by single-gene analysis and gene set analysis. The being up-regulated at high loss belong to groups genes functionally categorized membrane proteins, signal transduction, cell communication,...
Contents Phospholipase C zeta ( PLCz ) and cyclooxygenase isoenzyme type 2 COX‐2 are important in spermatogenesis, but their effect has not yet confirmed pigs. Therefore, this study was aimed to analyse association with sperm quality fertility identify the mRNA protein expression boars reproductive tissues. DNA samples from 231 Pietrain (PI) 109 × Hampshire (PIHA) pigs records of [sperm concentration (SCON), motility, semen volume, plasma droplet abnormal spermatozoa rate] (non‐return rate...
Summary Mannose‐binding lectin (MBL) mediates activation of the complement system via pathway. Two forms MBL, MBL‐A and MBL‐C, were characterized in rodents, rabbits, bovine rhesus monkeys, whereas only one form was identified humans, chimpanzees chickens. The two are encoded by distinct genes named MBL1 MBL2 , which have been many species including pig. In this report, we studied porcine . MBL had higher identities to rather than primate rodent sequences. Both assigned chromosome 14...
To overcome developmental problems as a consequence of single embryo culture, the Well (WOW) culture system has been developed. In this study, we aimed to examine effect densities with respect both microenvironment and macroenvironment on rates quality get deeper insight into developmentally important mechanisms. WOW diameter depth significantly affected (p < 0.05). WOWs 500 μm reached higher blastocyst (32.5 vs 21.1% 20.3%) compared embryos cultured in 300 or plain controls. Embryos 700...
The aim of the present study was to detect quantitative trait loci (QTL) for serum levels cytokines and Toll-like receptors as traits related innate immunity in pig. For this purpose, concentration interleukin 2 (IL2), 10 (IL10), interferon-gamma (IFNG), receptor (TLR2) 9 (TLR9) were measured blood samples obtained from F(2) piglets (n = 334) a Duroc × Piétrain resource population (DUPI) after Mycoplasma hypopneumoniae (Mh), tetanus toxoid (TT) Porcine Reproductive Respiratory Syndrome Virus...
The present study was undertaken to perform an inventory of pig production systems, at farm level. main aim document the variety existing systems in European countries. Information collected from available sources, mostly national experts and literature, regarding target markets information management, apparent degree intensification, technical economic performance, environmental impact animal welfare. Eighty-four were identified 23 countries, which 40 reported as conventional remaining 44...
Abstract Background Leg weakness issues are a great concern for the pig breeding industry, especially with regard to animal welfare. Traits associated leg partly influenced by genetic background of animals but basis these traits is not yet fully understood. The aim this study was identify quantitative trait loci (QTL) affecting in pigs. Methods Three hundred and ten F 2 pigs from Duroc × Pietrain resource population were genotyped using 82 markers. Front rear legs feet scores based on...
Contents ESR2 is involved in oestrogen‐related apoptosis cell cycle spermatogenesis but their effects have not yet confirmed pig. Therefore, this study was aimed to investigate the association of polymorphism with sperm quality and boar fertility traits analyse mRNA protein expressions reproductive tissues. DNA samples from 203 Pietrain (PI) 100 × Hampshire (PIHA) pigs records [sperm concentration (SCON), motility (MOT), semen volume (VOL), plasma droplet rate (PDR) abnormal spermatozoa...
This study was conducted to investigate the effect of suppressing transcription factor gene MSX1 on development in vitro produced bovine oocytes and embryos, identify its potential target genes regulated by this gene. Injection long double-stranded RNA (LdsRNA) small interfering (siRNA) at germinal vesicle stage oocyte reduced mRNA expression 73 37% respectively metaphase II compared with non-injected controls. Similarly, injection same anti-sense oligomers zygote 52 33% 8-cell Protein also...