A5076037101- Metabolomics and Mass Spectrometry Studies
- Advanced Chemical Sensor Technologies
- Laser Applications in Dentistry and Medicine
- Oral microbiology and periodontitis research
- Edible Oils Quality and Analysis
- Salivary Gland Disorders and Functions
- Electron Spin Resonance Studies
- NMR spectroscopy and applications
- Gout, Hyperuricemia, Uric Acid
- Dental Anxiety and Anesthesia Techniques
- Fatty Acid Research and Health
- Phytochemicals and Antioxidant Activities
- Lysosomal Storage Disorders Research
- Advanced MRI Techniques and Applications
- Free Radicals and Antioxidants
- Antioxidant Activity and Oxidative Stress
- Spectroscopy and Chemometric Analyses
- Dental Erosion and Treatment
- Trace Elements in Health
- Biochemical effects in animals
- Meat and Animal Product Quality
- Dental Health and Care Utilization
- Exercise and Physiological Responses
- Electrochemical Analysis and Applications
- Advanced NMR Techniques and Applications
De Montfort University
2016-2025
University of Maiduguri
2022
Tulane University
2022
St. Mary's Health System
2022
University of Warwick
2012-2021
Leicester College
2021
The Gate
2021
University of Nevada, Las Vegas
2020-2021
University of Kang Ning
2016
University of Bolton
2009-2013
The nature of non-transferrin-bound iron in the plasma or serum iron-overloaded hemochromatosis patients was studied by high performance liquid chromatography (HPLC) and resolution nuclear magnetic resonance (NMR).500-MHz proton Hahn spinecho NMR spectra serum, combined with use chelator desferrioxamine, suggests complexation ions citrate a possible involvement acetate.Addition FeCL to samples broadened signals from citrate.HPLC analysis rigorously confirmed presence an iron-citrate complex...
Allopurinol is a scavenger of the highly reactive hydroxyl radical ( k 2 approx. 10 9 M −1 s ). One product attack upon allopurinol oxypurinol, which major metabolite allopurinol. Oxypurinol better than 4 × ) and it also reacts with myeloperoxidase‐derived oxidant hypochlorous acid. Hence protective actions against reperfusion damage after hypoxia need not be entirely due to xanthine oxidase inhibition.
Attack by .OH radicals, generated a Fenton system, upon salicylate produces 2,3-dihydroxybenzoate and 2,5-dihydroxybenzoate as major products catechol minor product. H.p.l.c. separation combined with electrochemical detection was used to identify quantify in human plasma synovial fluid. We propose that conversion of into 2,3-dihydroxybenzoate, or other aromatic compounds specific hydroxylated products, may be useful assay for formation the body.
Free-radical attack upon uric acid generates allantoin [Ames, Cathcart, Schwiers & Hochstein (1981) Proc. Natl. Acad. Sci. U.S.A. 78, 6858-6862]. Methods are described for the accurate measurement of and in human body fluids. The concentrations serum synovial fluid reported. It is suggested that changes concentration may be a useful index free-radical reactions taking place vivo.
Ascorbic acid, at physiological concentrations, can scavenge the myeloperoxidase‐derived oxidant hypochlorous acid rates sufficient to protect α 1 ‐antiprotease against inactivation by this molecule. The rapid depletion of ascorbic sites inflammation, as in inflamed rheumatoid joint, may therefore facilitate proteolytic damage.
Proton Hahn spin-echo n.m.r. spectroscopy was employed to detect abnormal metabolites present in rheumatoid synovial fluid that are derived from the deleterious generation of reactive oxygen radical species during exercise inflamed joint. A resonance attributable a low-molecular-mass N-acetylglucosamine-containing oligosaccharide formed by oxygen-radical-mediated depolymerization synovial-fluid hyaluronate clearly demonstrable when subjects with inflammatory joint disease were exercised....
Thiourea and dimethylthiourea are powerful scavengers of hydroxyl radicals (.OH), has been used to test the involvement .OH in several animal models human disease. It is shown that both thiourea HOCl, a oxidant produced by neutrophil myeloperoxidase. Hence ability protect against neutrophil-mediated tissue damage cannot be as evidence for role causing such damage. Dimethyl sulphoxide also reacts with but at rate probably too low biologically significant dimethyl concentrations up 10 mM....
Thermal stressing of polyunsaturated fatty acid (PUFA)- rich culinary oils according to routine frying or cooking practices generates high levels cytotoxic aldehydic products (predominantly trans-2-alkenals, trans,trans-alka-2,4-dienals, cis,trans-alka-2, 4-dienals, and n-alkanals), species arising from the fragmentation conjugated hydroperoxydiene precursors. In this investigation we demonstrate that typical trans-2-alkenal compounds known be produced thermally induced autoxidation PUFAs...
Novel sensing technologies for liquid biopsies offer promising prospects the early detection of metabolic conditions through omics techniques. Indeed, high-field nuclear magnetic resonance (NMR) facilities are routinely used metabolomics investigations on a range biofluids in order to rapidly recognise unusual patterns patients suffering from diseases. However, these techniques restricted by prohibitively large size and cost such facilities, suggesting possible role smaller, low-field NMR...
Abstract Human ingestion of cytotoxic and genotoxic aldehydes potentially induces deleterious health effects, high concentrations these secondary lipid oxidation products (LOPs) are generated in polyunsaturated fatty acid (PUFA)-rich culinary oils during temperature frying practices. Here, we explored the peroxidative resistance a novel monounsaturate-rich algae oil (MRAFO) laboratory-simulated shallow- domestically-based repetitive deep-frying episodes (LSSFEs DBRDFEs respectively), latter...
We have explored the ability of high-resolution NMR techniques to (1) index salivary biomolecules and (2) provide valuable data regarding intra- inter-subject variability in concentrations a series components readily determinable by this technique (organic acids malodorous amines). Experiments were conducted on ‘whole’ saliva samples collected from 20 patients, either randomly during their daily activities, or, for investigations involving quantification biomolecules, immediately after they...
The oxidative deterioration of glycerol‐bound polyunsaturated fatty acids (PUFAs) in culinary oils and fats during episodes heating associated with normal usage (30–90 min at 180°C) has been monitored by high field 1 H NMR spectroscopy. Thermal stressing PUFA‐rich generated levels n ‐alkanals, trans ‐2‐alkenals, alka‐2,4‐dienals 4‐hydroxy‐ ‐2‐alkenals via decomposition their conjugated hydroperoxydiene precursors, whereas only low concentrations selected aldehydes were produced a PUFA...
The 1H Hahn spin-echo NMR profiles of rheumatoid synovial fluids have been investigated and compared with those matched serum samples. In addition to markedly elevated lactate diminished glucose concentrations, inflammatory contained (i) substantially lower levels NMR-detectable chylomicron-and very-low-density-lipoprotein-associated triacylglycerols which appear a shortened mean chain-length, (ii) high concentrations ketone bodies (predominantly 3-D-hydroxybutyrate), relative corresponding...