A5085349632- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- Light effects on plants
- Plant tissue culture and regeneration
- Plant Reproductive Biology
- Sirtuins and Resveratrol in Medicine
- Redox biology and oxidative stress
- Chromosomal and Genetic Variations
Sichuan Agricultural University
2020-2023
Abstract MYB transcription factors play important roles in plant responses to cold stress, but the associated underlying mechanisms remain unclear. In this study, a cold-induced factor, DgMYB2, was isolated from chrysanthemum (Chrysanthemum morifolium Ramat). DgMYB2 localized nucleus and exhibited transactivational activity. Overexpression of improved tolerance chrysanthemum, while antisense suppression lines decreased compared that wild type. Additionally, electrophoretic mobility shift...
Long noncoding RNAs (lncRNAs) are widely involved in the regulation of plant growth and development, but their mechanism action response to cold stress plants remains unclear. Here, we found an lncRNA transcribed from antisense strand DgTCP1 (class I Teosinte branched1/Cycloidea/Proliferating [TCP] transcription factor) chrysanthemum (Chrysanthemum morifolium Ramat.), named DglncTCP1. During stress, overexpression improved tolerance chrysanthemum, while editing line (dgtcp1) showed decreased...
The bZIP transcription factor plays a very important role in abiotic stresses, e.g. drought, salt, and low-temperature stress, but the mechanism of action at low temperature is still unclear. In this study, overexpression DgbZIP3 led to increased tolerance chrysanthemum (Chrysanthemum morifolium Ramat.) cold whereas antisense suppression resulted decreased tolerance. Electrophoretic mobility shift assay (EMSA), chromatin immunoprecipitation (ChIP), luciferase complementary imaging analysis...
Abstract Cold affects the growth and development of plants. MYB transcription factors histone H3K4me3 transferase ARABIDOPSIS TRITHORAXs (ATXs) play important regulatory functions in process plant resistance to low-temperature stress. In this study, DgMYB expression was responsive low temperature, overexpression led increased tolerance, whereas dgmyb mutant resulted decreased tolerance Chrysanthemum morifolium (Dendranthema grandiflorum var. Jinba) cold stresses. Interestingly, we found that...
Lysine crotonylation of proteins is a recently identified post-translational modification (PTM) in plants. However, the function lysine-crotonylated response to abiotic stress plants has not been reported. In this study, we temperature-induced lipocalin-1-like gene (DgTIL1) from chrysanthemum and showed that it was notably induced cold stress. Overexpression DgTIL1 enhanced tolerance transgenic chrysanthemum. Ubiquitin membrane yeast two-hybrid (MYTH) system bimolecular fluorescence...
Lysine crotonylation is a protein post-translational modification that has been newly discovered in recent years. There are few studies on the lysine of proteins plants, and their functions response to cold stress still unclear. In this study, chrysanthemum (Chrysanthemum morifolium Ramat.) glutathione peroxidase (GPX) gene was selected named DgGPX1, found be responsive low temperature. Overexpression DgGPX1 improved resistance transgenic by increasing GPX activity reduce accumulation...
Abstract Lysine malonylation (Kmal) is a recently discovered posttranslational modification, and its role in the response to abiotic stress has not been reported plants. In this study, we isolated nonspecific lipid transfer protein, DgnsLTP1, from chrysanthemum (Dendranthema grandiflorum var. Jinba). Overexpression CRISPR-Cas9–mediated gene editing of DgnsLTP1 demonstrated that protein endows with cold tolerance. Yeast 2-hybrid, bimolecular fluorescence complementation, luciferase...