A5050046573- Liver Disease Diagnosis and Treatment
- Liver physiology and pathology
- Immune cells in cancer
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- Immunotherapy and Immune Responses
- Cell Adhesion Molecules Research
- Sepsis Diagnosis and Treatment
- Phagocytosis and Immune Regulation
- Immune Cell Function and Interaction
- Wound Healing and Treatments
- Ultrasound and Hyperthermia Applications
- Chemokine receptors and signaling
- Circular RNAs in diseases
- 3D Printing in Biomedical Research
- Nanoplatforms for cancer theranostics
- Innovative Microfluidic and Catalytic Techniques Innovation
- Hepatitis C virus research
- Endoplasmic Reticulum Stress and Disease
- Immune Response and Inflammation
- Tissue Engineering and Regenerative Medicine
- Liver Disease and Transplantation
- Advanced biosensing and bioanalysis techniques
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
DWI – Leibniz Institute for Interactive Materials
2023-2025
RWTH Aachen University
2015-2025
Universitätsklinikum Aachen
2015-2025
Institute of Rheumatology
2025
Justus-Liebig-Universität Gießen
2022
Freie Universität Berlin
2022
Charité - Universitätsmedizin Berlin
2022
Case Western Reserve University
2019
Cornell University
2019
657 Oslo
2016
Abstract Background Recent experimental approaches have unraveled essential migratory and functional differences of monocyte subpopulations in mice. In order to possibly translate these findings into human physiology pathophysiology, subsets need be carefully revisited health disease. analogy murine studies, we hypothesized that dynamically change during ageing, potentially influencing their functionality contributing immunosenescence. Results Circulating subsets, surface marker chemokine...
Abstract Non‐alcoholic fatty liver disease ( NAFLD ) represents the most common in Western countries and often progresses to non‐alcoholic steatohepatitis NASH leading ultimately fibrosis cancer. The occurrence of hepatocyte cell death—so far characterized as apoptosis—represents a fundamental step from benign steatosis toward progressive steatohepatitis. In contrast, function RIP 3‐dependent “necroptosis” ‐induced is currently unknown. We show that 3 upregulated human dietary mouse model...
<h3>Objectives</h3> In chronic liver injury, angiogenesis, the formation of new blood vessels from pre-existing ones, may contribute to progressive hepatic fibrosis and development hepatocellular carcinoma. Although hypoxia-induced expression vascular endothelial growth factor (VEGF) occurs in advanced fibrosis, we hypothesised that inflammation endorse angiogenesis already at early stages fibrosis. <h3>Design</h3> Angiogenesis livers c57BL/6 mice upon carbon tetrachloride- or bile duct...
Macrophages constitute a major proinflammatory component during chronic liver diseases and are considered key factor in promoting hepatic fibrosis. However, there is increasing evidence that distinct monocyte macrophage subsets exert critical functions regression from organ fibrosis as well. Experimental mouse models of have identified “restorative” macrophages Ly-6C (Ly6C, Gr1) low-expressing, monocyte-derived cells. We investigated molecular pathways balancing restorative well...
Nanoparticle-based in vivo applications should consider the omnipresence of phagocytes bloodstream and tissue. We have studied nanoparticle uptake capacities most important human primary leukocyte populations using a library encompassing both rod-shaped spherical gold nanoparticles with diameters between 15 50 nm variety surface chemistries. Cetyltrimethylammoniumbromide (CTAB)-stabilized were internalized rapidly within min large amounts by macrophages to lower extent also monocytes....
It has recently been discovered that human immune cells, especially neutrophil granulocytes, form extracellular traps (NETs) abolish pathogens. Our study provides evidence formed by neutrophils, monocytes and macrophages act as physical barriers for nanoparticles, thus presenting a new nanomaterial clearance mechanism of the system. While particle shape is minor importance, positive charges significantly enhance trapping.
Chemokines critically control the infiltration of immune cells upon liver injury, thereby promoting hepatic inflammation and fibrosis. The chemokine receptor CCR8 can affect trafficking monocytes/macrophages, monocyte-derived dendritic (DCs) T-helper cell (Th) subsets, but its role in diseases is currently unknown. To investigate functional diseases, ccr8 −/− wild-type (WT) mice were subjected to chronic experimental injury models carbon tetrachloride (CCl4) administration surgical bile duct...
Targeted nanomedicine holds enormous potential for advanced diagnostics and therapy. Although it is known that nanoparticles accumulate in liver vivo, the impact of cell-targeting particles on liver, especially disease conditions, largely obscure. We had previously demonstrated peptide-conjugated differentially macrophage activation vitro. thus comprehensively studied distribution gold nanorods (AuNR) mice vivo assessed their hepatotoxicity systemic hepatic immune cells healthy animals...
Background & AimsHepatocellular carcinoma (HCC) typically arises in fibrotic or cirrhotic livers, which are characterized by pathogenic angiogenesis. Myeloid immune cells, specifically tumor-associated macrophages (TAMs), may represent potential novel therapeutic targets HCC, complementing current ablative therapies. However, the detailed functions of TAM subsets hepatocarcinogenesis have remained obscure.MethodsTAM were analyzed in-depth human HCC samples and a combined fibrosis–HCC mouse...
Pathogen‐ and injury‐related danger signals as well cytokines released by immune cells influence the functional differentiation of macrophages in chronic inflammation. Recently, liver‐derived plasma protein, histidine‐rich glycoprotein (HRG), was demonstrated, mouse tumor models, to mediate transition alternatively activated (M2) proinflammatory (M1) macrophages, which limit growth metastasis. We hypothesized that HRG is a critical endogenous modulator hepatic macrophage functionality...
Receptor-interacting protein kinase 3 (RIPK3) mediates necroptosis, a form of programmed cell death that promotes inflammation in various pathological conditions, suggesting it might be privileged pharmacological target. However, its function glucose homeostasis and obesity has been unknown. Here we show RIPK3 is over expressed the white adipose tissue (WAT) obese mice fed with choline-deficient high-fat diet. Genetic inactivation Ripk3 increased Caspase-8-dependent adipocyte apoptosis WAT...
Lymphopenia and functional defects in lymphocytes may impact the prognosis patients with critical illness or sepsis. Therefore, we prospectively analyzed peripheral blood leukocytes from 63 healthy volunteers, 50 non-critically ill standard care (SC) infections, 105 intensive unit (ICU) (52 sepsis, 53 without sepsis) using flow cytometry. Compared to SC ICU showed significant leukocytosis, especially while lymphocyte numbers were significantly decreased. All major populations (B, T, natural...
Hepatic clearance of lipid nanoparticles (LNP) with encapsulated nucleic acids restricts their therapeutic applicability. Therefore, tools for regulating hepatic are high interest acid delivery. To this end, work employs wild-type (WT) and low-density lipoprotein receptor (Ldlr)
Injectable poly-L-lactic acid (PLLA-SCA) is used for the correction of shallow to deep nasolabial fold contour deficiencies, cheek wrinkles, and other facial wrinkles. In contrast hyaluronan (HA) fillers, PLLA-SCA has a biostimulatory effect by activating resident fibroblasts produce collagen, but mechanisms are not known in detail at molecular level. Therefore, our aim was investigate effects comprehensive vitro study. Since PLLA-SCA-dependent collagen production depends on interaction with...
Circulating levels of soluble urokinase plasminogen activation receptor (suPAR) have been proposed as a prognostic biomarker in patients with critical illness and sepsis. However, the origin suPAR sepsis has remained obscure. We investigated potential cellular sources by analyzing membrane-bound activator (uPAR, CD87) evaluated its clinical relevance critically ill patients. studied 87 (44 sepsis, 43 without sepsis) from medical intensive care unit (ICU) comparison to 48 standard infections...