A5080547230- HIV Research and Treatment
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA Research and Splicing
- Cytomegalovirus and herpesvirus research
- Bacteriophages and microbial interactions
- HIV/AIDS drug development and treatment
The Ohio State University
2021-2025
The average eukaryotic transfer ribonucleic acid (tRNA) contains 13 post-transcriptional modifications; however, their functional impact is largely unknown. Our understanding of the complex tRNA aminoacylation machinery in metazoans also remains limited. Herein, using a series high-resolution cryo-electron microscopy (cryo-EM) structures, we provide mechanistic basis for recognition and fully modified cellular tRNALys3 by human lysyl-tRNA synthetase (h-LysRS). anticodon loop modifications...
Interactions between lysyl-tRNA synthetase (LysRS) and HIV-1 Gag facilitate selective packaging of the reverse transcription primer, tRNA
Abstract Background During HIV-1 maturation, Gag and Gag-Pol polyproteins are proteolytically cleaved the capsid protein polymerizes to form honeycomb lattice. integrase (IN) binds viral genomic RNA (gRNA) impairment of IN-gRNA binding leads mis-localization nucleocapsid (NC)-condensed ribonucleoprotein complex outside core. IN NC were previously demonstrated bind gRNA in an orthogonal manner virio; however, effect alone or simultaneous both proteins on structure is not yet well understood....
ABSTRACT The average eukaryotic tRNA contains 13 posttranscriptional modifications; however, their functional impact is largely unknown. Our understanding of the complex aminoacylation machinery in metazoans also remains limited. Herein, using a series high-resolution cryo-electron microscopy (cryo-EM) structures, we provide mechanistic basis for recognition and fully-modified cellular Lys3 by human lysyl-tRNA synthetase (h-LysRS). anticodon loop modifications S34 (mcm 5 s 2 U) R37 (ms t 6...