A5025135852- Pluripotent Stem Cells Research
- Advanced Fluorescence Microscopy Techniques
- Neuroscience and Neural Engineering
- 3D Printing in Biomedical Research
- Cell Image Analysis Techniques
- Neurogenesis and neuroplasticity mechanisms
- Autism Spectrum Disorder Research
- 14-3-3 protein interactions
- Cellular Mechanics and Interactions
- Dementia and Cognitive Impairment Research
- Alzheimer's disease research and treatments
- Genetics, Aging, and Longevity in Model Organisms
- Advanced Electron Microscopy Techniques and Applications
- RNA Research and Splicing
University Hospital Bonn
2020-2023
University of Bonn
2017-2022
Miller-Dieker syndrome (MDS) is caused by a heterozygous deletion of chromosome 17p13.3 involving the genes LIS1 and YWHAE (coding for 14.3.3ε) leads to malformations during cortical development. Here, we used patient-specific forebrain-type organoids investigate pathological changes associated with MDS. Patient-derived are significantly reduced in size, change accompanied switch from symmetric asymmetric cell division ventricular zone radial glia cells (vRGCs). Alterations microtubule...
The human cortex is highly expanded and exhibits a complex structure with specific functional areas, providing higher brain function, such as cognition. Efforts to study cerebral development have been limited by the availability of model systems. Translating results from rodent studies system restricted species differences on primary tissues are hampered lack tissue well ethical concerns. Recent in pluripotent stem cell (PSC) technology include generation three-dimensional (3D)...
ABSTRACT Organoids are stem cell-derived three-dimensional cultures offering a new avenue to model human development and disease. Brain organoids allow the study of various aspects brain in finest details vitro tissue-like context. However, spatial relationships subcellular structures, such as synaptic contacts between distant neurons, hardly accessible by conventional light microscopy. This limitation can be overcome systems that quickly image entire organoid three dimensions...
The human cortex is highly expanded and exhibits a complex structure with specific functional areas, providing higher brain function, such as cognition. Efforts to study cerebral development have been limited by the availability of model systems. Translating results from rodent studies system restricted species differences on primary tissues are hampered lack tissue well ethical concerns. Recent in pluripotent stem cell (PSC) technology include generation three-dimensional (3D)...
Abstract We combine tissue expansion and light sheet fluorescence microscopy to analyze brain organoids. It is possible zoom from a mesoscopic overview super-resolution in single imaging session, revealing cellular subcellular structural details, such as the positions spacing of pre- postsynaptic proteins. Light (LSFEM) greatly facilitates qualitative quantitative use organoids developmental disease-related studies.
Abstract Organoids are human stem cell-derived three-dimensional cultures offering a new avenue to model development and disease. Brain organoids allow studying various aspects of brain in the finest details vitro tissue-like context. However, spatial relationships subcellular structures such as synaptic contacts between distant neurons hardly accessible by conventional light microscopy. This limitation can be overcome systems that quickly image entire organoid three dimensions...