A5081030176- Single-cell and spatial transcriptomics
- CRISPR and Genetic Engineering
- Developmental Biology and Gene Regulation
- Zebrafish Biomedical Research Applications
- HER2/EGFR in Cancer Research
- Lung Cancer Treatments and Mutations
- Statistical Methods in Clinical Trials
- Lung Cancer Research Studies
- Reliability and Agreement in Measurement
- Service-Oriented Architecture and Web Services
- Statistical Methods and Bayesian Inference
- Customer churn and segmentation
- Advanced biosensing and bioanalysis techniques
- Cell Image Analysis Techniques
Columbia University
2022-2025
Daiichi Sankyo (United States)
2021
Fred Hutch Cancer Center
2007
The evolution of advanced cognition in vertebrates is associated with two independent innovations the forebrain: six-layered neocortex mammals and dorsal ventricular ridge (DVR) sauropsids (reptiles birds). How these arose vertebrate ancestors remains unclear. To reconstruct forebrain tetrapods, we built a cell-type atlas telencephalon salamander Pleurodeles waltl . Our molecular, developmental, connectivity data indicate that parts sauropsid DVR trace back to tetrapod ancestors. By...
Unlike sequencing-based methods, which require cell lysis, optical pooled genetic screens enable investigation of spatial phenotypes, including morphology, protein subcellular localization, cell–cell interactions and tissue organization, in response to targeted CRISPR perturbations. Here we report a multimodal screening method, call CRISPRmap. CRISPRmap combines situ guide-identifying barcode readout with multiplexed immunofluorescence RNA detection. Barcodes are detected read out through...
Pooled genetic screens are powerful tools to study gene function in a high-throughput manner. Typically, sequencing-based require cell lysis, which limits the examination of critical phenotypes such as morphology, protein subcellular localization, and cell-cell/tissue interactions. In contrast, emerging optical pooled screening methods enable investigation these spatial response targeted CRISPR perturbations. this study, we report multi-omic method, have named CRISPRmap. Our method combines...
Abstract The evolution of advanced cognition in vertebrates is associated with two independent innovations the forebrain: six-layered neocortex mammals and dorsal ventricular ridge (DVR) sauropsids (reptiles birds). How these novelties arose vertebrate ancestors remains unclear. To reconstruct forebrain tetrapods, we built a cell type atlas telencephalon salamander Pleurodeles waltl . Our molecular, developmental, connectivity data indicate that parts sauropsid DVR trace back to tetrapod...
With the improvement of consumers' personalized demand, each website provides consumers with a more convenient way aggregation and information technology, which challenges traditional enterprise-oriented e-commerce model creates new customer-centered unbounded contact model. This paper analyzes background application mode, discusses its possible development in future.