A5009472929- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Viral Infections and Immunology Research
- COVID-19 epidemiological studies
- Animal Virus Infections Studies
- COVID-19 diagnosis using AI
- COVID-19 and Mental Health
- Advanced Chemical Sensor Technologies
- Vaccine Coverage and Hesitancy
- Animal Disease Management and Epidemiology
- Infection Control and Ventilation
- Data-Driven Disease Surveillance
Stanford University
2021-2025
Palo Alto University
2022
Polo d’Innovazione di Genomica
2021
Abstract Background Preventing vaccine-derived polio is crucial, yet evolution and transmission mechanisms remain unclear. Our previous study in Mayan infants found that preexisting OPV-induced antibodies did not affect S3 immunogenicity. We now explore if viral mutations differ those with without antibodies. Using NGS, we identify characterize vaccine poliovirus’ mutation patterns from stool sample isolates post one two OPV doses. hypothesize intestinal immunity will correlate increased...
<h3>Importance</h3> There is an urgent need to assess the feasibility of COVID-19 surveillance measures in educational settings. <h3>Objective</h3> To whether young children can feasibly self-collect SARS-CoV-2 samples for testing over course academic year. <h3>Design, Setting, and Participants</h3> This prospective pilot cohort study was conducted from September 10, 2020, June 2021, at a K-8 school San Mateo County, California. The research consisted quantitative data collection efforts:...
Importance The COVID-19 pandemic has led to 775 million documented cases and over 7 deaths worldwide as of March 2024 is an ongoing health crisis. To limit viral spread within households in the community, public officials have recommended self-isolation, self-quarantine exposed household contacts, mask use. Yet, risk transmission (HHT) may be underestimated due low frequency sampling, factors for HHT are not well understood. Objectives estimate secondary attack rate SARS-CoV-2 define new...
The limited variation observed among severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) consensus sequences makes it difficult to reconstruct transmission linkages in outbreak settings. Previous studies have recovered within individual SARS-CoV-2 infections but not yet measured the informativeness of within-host for inference.
There is an increasing burden of circulating vaccine-derived polioviruses (cVDPVs) due to the continued use oral poliovirus vaccine (OPV). However, informativeness routine OPV VP1 sequencing for early identification viruses carrying virulence-associated reversion mutations has not been directly evaluated in a controlled setting. We prospectively collected 15,331 stool samples track shedding from children receiving and their contacts ten weeks following immunization campaign Veracruz State,...
Abstract Background The limited variation observed among SARS-CoV-2 consensus sequences makes it difficult to reconstruct transmission linkages in outbreak settings. Previous studies have recovered within individual infections but not yet measured the informativeness of within-host for inference. Methods We performed tiled amplicon sequencing on 307 samples from four prospective and combined sequence data with household membership data, a proxy linkage. Results Consensus households had...
Abstract COVID-19 testing is not accessible for millions during this pandemic despite our best efforts. Without greatly expanded of asymptomatic individuals, contact tracing and subsequent isolation spreaders remains as a means control. In an effort to increase RT-PCR assay the presence novel beta-coronavirus SARS-CoV-2 well improve sample collection safety, GenTegra LLC has introduced two products saliva viral RNA stabilization: GTR-STM™ (GenTegra Saliva Transport Medium) GTR-STMdk™ Medium...
Abstract Background In order to mitigate the spread of SARS-CoV-2 and COVID-19 pandemic, public health officials have recommended self-isolation, self-quarantine exposed household contacts (HHC), mask use limit viral within households communities. While transmission is common, risk factors for HHC are poorly understood. Methods this prospective cohort study, we enrolled 37 with at least one reverse transcription polymerase chain reaction-confirmed (RT-PCR) index case from March 2020 - 2021,...
There is an increasing burden of circulating vaccine-derived polioviruses (cVDPVs) due to the continued use oral poliovirus vaccine (OPV). However, informativeness routine OPV VP1 sequencing for early identification viruses carrying virulence-associated reversion mutations has not been directly evaluated in a controlled setting. We prospectively collected 15,331 stool samples track shedding from vaccinated children and their contacts ten weeks following immunization campaign Veracruz State,...
Abstract Background As wild poliovirus is eradicated, preventing circulation of vaccine-derived top priority. Our lab developed real-time multiplex PCR and deep sequencing methods to detect characterize OPV strains directly from stool samples. This method requires gel purification product created viral RNA in However, causes significant genetic material loss time consuming. Here, we explore combinations different NGS optimize sequencing. Methods 554 positive samples a previous study were...
Abstract Background Breath samples collected from patients infected with respiratory viruses are necessary for viral detection using breath analyzer devices. Given the highly transmissible nature of many these illnesses, sample collection requires a multi-layered approach to ensure safety research staff responsible obtaining and transporting samples. Our team established protocol minimize exposure transmission COVID-19 when collecting Methods We 64 participants, which 31 (48.4%) were...
Abstract Background Understanding how COVID-19 vaccination affects transmission of SARS-CoV-2 within households may affect policy and healthcare decisions. We hypothesized that reduces viral load in vaccinated household members. Methods prospectively enrolled participants during March 2020 – October 2021. Index cases (IC) were eligible if they tested positive for the previous 10 days did not have contacts (HC) who had or symptoms COVID-19. Participants self-collected anterior nares swabs...
Abstract Background As wild poliovirus is eradicated, preventing circulation of vaccine-derived top priority. Our lab developed real-time multiplex PCR assays and deep sequencing methodology to detect characterize OPV strains from stool samples. The method requires gel purification product created viral RNA in However, the process filters out a significant portion Here, we compare gel- vs. DNA- sample retainment for downstream analysis. Methods 554 samples qPCR positive at least one serotype...
Abstract Background While pediatric cases of COVID-19 are at low risk for adverse events, schoolchildren should be considered surveillance as they can become infected school and serve sources household or community transmission. Our team assessed the feasibility young children self-collecting SARS-CoV-2 samples testing in an educational setting. Methods Students a K-8 were tested weekly from September 2020 - June 2021. Error rates collected January Clinical staff provided all students with...