A5036784736- Advanced Fluorescence Microscopy Techniques
- Cellular Mechanics and Interactions
- Angiogenesis and VEGF in Cancer
- Lipid Membrane Structure and Behavior
- Hippo pathway signaling and YAP/TAZ
- Cytokine Signaling Pathways and Interactions
- Cell Adhesion Molecules Research
- Cellular transport and secretion
- Cardiovascular Health and Disease Prevention
- Nuclear Structure and Function
- Blood properties and coagulation
- Near-Field Optical Microscopy
- Monoclonal and Polyclonal Antibodies Research
- Protein Structure and Dynamics
- CRISPR and Genetic Engineering
- Muscle Physiology and Disorders
- Microtubule and mitosis dynamics
- Cell Image Analysis Techniques
- Zebrafish Biomedical Research Applications
- Orbital Angular Momentum in Optics
- Erythrocyte Function and Pathophysiology
- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Planarian Biology and Electrostimulation
- Endoplasmic Reticulum Stress and Disease
University of Lisbon
2015-2022
Champalimaud Foundation
2020-2022
Korea Advanced Institute of Science and Technology
2019
Leiden University
2009-2015
Morphogenesis of hierarchical vascular networks depends on the integration multiple biomechanical signals by endothelial cells, cells lining interior blood vessels. Expansion arises through sprouting angiogenesis, a process involving extensive cell rearrangements and collective migration. Yet, mechanisms controlling angiogenic behavior remain poorly understood. Here, we show this is regulated non-canonical Wnt signaling. We identify that Wnt5a specifically activates Cdc42 at junctions...
Abstract Despite promising preclinical results, average response rates to anti-VEGF therapies, such as bevacizumab, are reduced for most cancers, while incurring in remarkable costs and side effects. Currently, there no biomarkers available select patients that can benefit from this therapy. Depending on the individual tumor, therapies either block or promote metastasis. In context, an assay able predict responses prior treatment, including impact metastasis would prove of great value guide...
Skeletal muscle myofibers are large and elongated cells with multiple evenly distributed nuclei. Nuclear distribution suggests that each nucleus influences a specific compartment within the myofiber implies functional role for nuclear positioning. Compartmentalization of mRNAs proteins has been reported at neuromuscular myotendinous junctions, but mRNA in non-specialized regions remains largely unexplored. We report bulk enriched around origin this perinuclear accumulation depends on...
Blood-vessel formation generates unique vascular patterns in each individual. The principles governing the apparent stochasticity of this process remain to be elucidated. Using mathematical methods, we find that transition between two fundamental morphogenetic programs—sprouting angiogenesis and remodeling—is established by a shift collective front-to-rear polarity endothelial cells mouse retina. We demonstrate competition biochemical (VEGFA) mechanical (blood-flow-induced shear stress) cues...
In this article, we present PolNet, an open-source software tool for the study of blood flow and cell-level biological activity during vessel morphogenesis. We provide image acquisition, segmentation, analysis protocol to quantify endothelial cell polarity in entire vivo vascular networks. combination, use computational fluid dynamics characterize hemodynamics networks under study. The enables, our knowledge first time, a network-level individual cells. To date, PolNet has proven invaluable...
Abstract Nuclear position is central to cell polarization, and its disruption associated with various pathologies. The nucleus moved away from the leading edge of migrating cells through connection moving dorsal actin cables, absence connections immobile ventral stress fibers. It unclear how these asymmetric nucleo-cytoskeleton are established. Here, using an in vitro wound assay, we find that remodeling endoplasmic reticulum (ER) impacts nuclear positioning formation a barrier shields ER...
Summary Cell migration is essential during development, regeneration, homeostasis, and disease. Depending on the microenvironment, cells use different mechanisms to migrate. Yet, all modes of require establishment an intracellular front–rear polarity axis for directional movement. Although can be easily identified in vitro conditions, its assessment vivo by live‐imaging challenging due tissue complexity lack reliable markers. Here, we describe a novel unique double fluorescent reporter mouse...
Research on trypanosomes as a model organism has provided substantial contribution to detailed understanding of basic cellular processes within the last few years. At same time, major advances in super-resolution microscopy have been achieved, facilitating resolution biological structures living cells at scale nm. However, motility prevented access high live cells. Here, we present hydrogel based poly(ethylene glycol) functionalized with either norbornene or thiol moieties for UV induced...
Blood vessel formation generates unique vascular patterns in each individual. The principles governing the apparent stochasticity of this process remain to be elucidated. Using mathematical methods, we find that transition between two fundamental morphogenetic programs – sprouting angiogenesis and remodeling is established by a shift on collective front-rear polarity endothelial cells. We demonstrate competition biochemical (VEGFA) mechanical (blood flow-induced shear stress) cues controls...
Abstract In this paper, we present PolNet, an open source software tool for the study of blood flow and cell-level biological activity during vessel morphogenesis. We provide image acquisition, segmentation, analysis protocol to quantify endothelial cell polarity in entire vivo vascular networks. combination, use computational fluid dynamics characterise haemodynamics networks under study. The enables, first time, network-level individual cells. To date, PolNet has proven invaluable...
Abstract Blood vessel formation generates unique vascular patterns in each individual. The principles governing the apparent stochasticity of this process remain to be elucidated. Using mathematical methods, we find that transition between two fundamental morphogenetic programs – sprouting angiogenesis and remodeling is established by a shift on collective front-rear polarity endothelial cells. We demonstrate competition biochemical (VEGFA) mechanical (blood flow-induced shear stress) cues...