A5073024588- Brucella: diagnosis, epidemiology, treatment
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- Monoclonal and Polyclonal Antibodies Research
- Cytokine Signaling Pathways and Interactions
- interferon and immune responses
- Escherichia coli research studies
- Identification and Quantification in Food
- RNA and protein synthesis mechanisms
- Cancer Cells and Metastasis
- Viral Infections and Immunology Research
- Toxin Mechanisms and Immunotoxins
- T-cell and B-cell Immunology
- Blood disorders and treatments
- Antibiotic Resistance in Bacteria
- Salmonella and Campylobacter epidemiology
- Genetics, Bioinformatics, and Biomedical Research
- Gene Regulatory Network Analysis
- Genomics and Phylogenetic Studies
- Neutropenia and Cancer Infections
- Burkholderia infections and melioidosis
- Cell Image Analysis Techniques
- bioluminescence and chemiluminescence research
- Synthesis and Characterization of Heterocyclic Compounds
- Galectins and Cancer Biology
Malek Ashtar University of Technology
2016-2020
Tarbiat Modares University
2010
An aequorin-based Escherichia coli strain JM109 biosensor was constructed and characterized for its potential to detect toluene related compounds in aqueous solutions. The based on a PGL2 plasmid carrying the lower pathway promoter (Pu) of xyl operon Pseudomonas putida mt-2, which incorporated with transcriptional activator xylR fused aequorin cDNA named pGL2-aequorin. Binding protein subset toluene-like activates transcription at Pu promoter, thus expression is controlled by Pu. In this...
Background: Zot (Zonula occludens toxin) is one of the secretion toxins Vibrio cholerae in small intestine that binds to certain receptors epithelial cells and causes a change structure tight junction.The purpose this research rapid detection zot enterotoxin gene using PCR. Materials Methods:The genomic DNA was extracted by isolation kit amplification carried out gene-specific primers.Then, PCR products were investigated electrophoresis on 1.2% agarose gel stained ethidium bromide.Also,...
Background: Human IFN-α is a family of structurally related proteins that exhibit wide range antiproliferative and antiviral activities. IFNs bind to specific cell receptor as the IFN-α/β (IFNAR) compose IFNAR1 IFNAR2 chains. Interferon’s affinity for chains changes type its activity. Objective: To understand structural basis these different activities, some important amino acid residues in this study binding region interferon were replaced more effective ones. Method: In order suggestion...
Introduction of a site-directed mutation can be effective method to evaluate properties various genes. Brucellosis is one the most common zoonotic infectious diseases, which causes great economic losses. Thus, determination pathogenicity factors in genus Brucella lead control this health problem. Due importance mutations identification genomic structure, overlap extension polymerase chain reaction (PCR) has been introduced as an improved technique for removal and replacement gene targets. In...
Background: Using Brucella abortus Strain 19 (S19) to control bovine brucellosis is restricted due induce antibodies the O-side chain of smooth lipopolysaccharide (LPS) which may be difficult differentiate vaccinated and infected animals. Furthermore, it virulent for humans can abortion cattle. Objectives: The aim this study was employ gene knockout B. S19 first time eliminate diagnostic defects obtain attenuated mutant strain. Material Methods: wbkA gene, one LPS O-chain coding genes,...
Type I interferons (IFNs) are homologous cytokines that bind to a cell surface receptor and establish signaling pathways motivate immune responses. The purpose of the current study is assess activity novel-engineered IFN-α2b. crystallographic structure IFN-α2b its receptors was acquired from Protein Data Bank. Various amino acid substitutions were designed based on structural properties other biological characteristics residues find most effective IFN affinity advanced activities. mutants...