A5020577329- Advanced MRI Techniques and Applications
- Magnetic and Electromagnetic Effects
- Lanthanide and Transition Metal Complexes
- Synthesis and biological activity
- Photoreceptor and optogenetics research
- Ultrasound and Hyperthermia Applications
- Advanced Neuroimaging Techniques and Applications
- Characterization and Applications of Magnetic Nanoparticles
- MRI in cancer diagnosis
- Cancer therapeutics and mechanisms
- Geomagnetism and Paleomagnetism Studies
- Software Engineering Techniques and Practices
- Microtubule and mitosis dynamics
- Diamond and Carbon-based Materials Research
- Nuclear Physics and Applications
- Mechanical and Optical Resonators
- Image Processing and 3D Reconstruction
- Electron Spin Resonance Studies
- thermodynamics and calorimetric analyses
- Neural dynamics and brain function
- Bioactive Compounds and Antitumor Agents
- Advanced NMR Techniques and Applications
- Protist diversity and phylogeny
- Heat shock proteins research
- NMR spectroscopy and applications
Harvard University
2025
Tennessee Technological University
2023
California Institute of Technology
2016-2023
Division of Chemistry
2016
Microbial rhodopsin–derived genetically encoded voltage indicators (GEVIs) are powerful tools for mapping bioelectrical dynamics in cell culture and live animals. Förster resonance energy transfer (FRET)–opsin GEVIs use voltage-dependent quenching of an attached fluorophore, achieving high brightness, speed, sensitivity. However, the sensitivity most FRET-opsin has been reported to decrease or vanish under two-photon (2P) excitation. Here, we investigated photophysics Voltron1 Voltron2. We...
Magnetic resonance imaging (MRI) is a widely used biomedical modality that derives much of its contrast from microscale magnetic field gradients in biological tissues. However, the connection between these sub-voxel patterns and MRI has not been studied experimentally. Here, we describe new method to map subcellular fields mammalian cells tissues using nitrogen vacancy diamond magnetometry connect maps voxel-scale contrast, providing insights for vivo agent design.
Dendrites on neurons support electrical excitations, but the computational significance of these events is not well understood. We developed molecular, optical, and tools for all-optical electrophysiology in dendrites. mapped sub-millisecond voltage dynamics throughout dendritic trees CA1 pyramidal under diverse optogenetic synaptic stimulus patterns, acute brain slices. Our data show history-dependent spike back-propagation distal dendrites, driven by locally generated Na+ spikes (dSpikes)....
Abstract Non-invasive imaging of gene expression in live, optically opaque animals is important for multiple applications, including monitoring genetic circuits and tracking cell-based therapeutics. Magnetic resonance (MRI) could enable such with high spatiotemporal resolution. However, existing MRI reporter genes based on metalloproteins or chemical exchange probes are limited by their reliance metals relatively low sensitivity. Here we introduce a new class reporters the human water...
Measuring cellular and tissue mechanics inside intact living organisms is essential for interrogating the roles of force in physiological disease processes. Current agents studying mechanobiology intact, are limited by poor light penetration material stability. Magnetomotive ultrasound an emerging modality real-time vivo imaging mechanics. Nonetheless, it has sensitivity spatiotemporal resolution. Here we describe magneto-gas vesicles (MGVs), protein nanostructures based on gas magnetic...
Topoisomerases, common targets for anti-tumor therapeutics, are crucial enzymes DNA replication, transcription, and many other aspects of metabolism. The potential anti-cancer effect thiosemicarbazones (TSC) metal-TSC complexes has been demonstrated by targeting several biological processes, including Human topoisomerases were discovered among the molecular TSCs, metal-chelated TSCs specifically displayed significant inhibition. processes which metal-TSCs or inhibit still being studied. In...
Abstract Making cells magnetic is a long‐standing goal of chemical biology, aiming to enable the separation from complex biological samples and their visualization in vivo using resonance imaging (MRI). Previous efforts towards this goal, focused on engineering biomineralize superparamagnetic or ferromagnetic iron oxides, have been largely unsuccessful due stringent required conditions. Here, we introduce an alternative approach making magnetic, biochemically maximizing cellular...
Abstract Making cells magnetic is a long‐standing goal of chemical biology, aiming to enable the separation from complex biological samples and their visualization in vivo using resonance imaging (MRI). Previous efforts towards this goal, focused on engineering biomineralize superparamagnetic or ferromagnetic iron oxides, have been largely unsuccessful due stringent required conditions. Here, we introduce an alternative approach making magnetic, biochemically maximizing cellular...
Abstract Measuring cellular and tissue mechanics inside intact living organisms is essential for interrogating the roles of force in physiological disease processes, a major goal field mechanobiology. However, existing biosensors 3D mechanics, primarily based on fluorescent emissions deformable materials, are limited vivo measurement due to light penetration poor material stability intact, organisms. While magneto-motive ultrasound (MMUS), which uses superparamagnetic nanoparticles as...
We demonstrate the possibility of reconstructing images from fNIRS brain activity and start building a prototype to match required specs. By training an image reconstruction model on downsampled fMRI data, we discovered that cm-scale spatial resolution is sufficient for generation. obtained 71% retrieval accuracy with 1-cm resolution, compared 93% full-resolution fMRI, 20% 2-cm resolution. With simulations high-density tomography, found time-domain can achieve continuous-wave fNIRS. Lastly,...
ABSTRACT The ability to monitor gene expression in intact, optically opaque animals is important for a multitude of applications including longitudinal imaging transgene and long term tracking cell based therapeutics. Magnetic resonance (MRI) could enable such monitoring with high spatial temporal resolution. However, existing MRI reporter genes, primarily on metal-binding proteins or chemical exchange saturation transfer probes, are limited by their reliance metal ions relatively low...