A high-performance thin-layer chromatographic method for simultaneous determination of nadifloxacin, mometasone furoate, and miconazole nitrate was developed validated as per International Conference on Harmonization guidelines. High-performance separation performed aluminum plates precoated with silica gel 60F254 methanol:ethyl acetate:toluene: acetonitrile:3M ammonium formate in water (1:2.5:6.0:0.3:0.2, % v/v) optimized mobile phase at detection wavelength 224 nm. The retardation factor...

We report herein, an accurate, precise, specific, and robust high-performance thin layer chromatographic technique along with forced degradation testing further validate it for the analysis of Bisoprolol fumarate Telmisartan in formulation. Robustness study was conducted by fractional factorial design (24−1) examined at both high level (+1) low (−1). Four factors were selected matrix: chamber saturation time, solvent front, wavelength, Toluene volume mobile phase. Base, acid, oxidation, dry...

ABSTRACT High‐performance liquid chromatography method for the estimation of chlorthalidone, cilnidipine, and bisoprolol fumarate in synthetic mixture simultaneously was developed validated using analytical quality by design. The mobile phase optimized Box–Behnken design selecting three factors optimization chromatographic conditions: flow rate, acetonitrile volume, pH buffer. International Council on Harmonisation Q2(R1) guideline used validation. Linearity fumarate, cilnidipine obtained...

Allergic diseases, such as allergic asthma, are hypersensitivity reactions initiated by immunological mechanisms. Myrica esculenta (M. esculenta) is known traditionally in Ayurveda to possess anti-asthmatic activity. The present investigation was undertaken evaluate the effect of crude extract stem bark M. (Family Myricaceae, commonly Kaiphal) on experimental reactions. Experimental models studied were pleurisy and vascular permeability induced acetic acid mice. Pretreatment with (75 mg/kg...

The present study examines simultaneous multiple response optimization using Derringer's desirability function for the development of an HPTLC method to detect Clonazepam and Paroxetine hydrochloride in pharmaceutical dosage form. Central composite design (CCD) was used optimize chromatographic conditions HPTLC. independent variables were n-butanol content mobile phase, chamber saturation time distance travelled. separation performed on aluminium plates pre-coated with silica gel 60 F254 as...

A sensitive, selective, precise and accurate stability-indicating high-performance thin layer chromatographic method for analysis of Ivabradine hydrochloride (IH) an anti anginal agent, both as a bulk drug in formulations was developed validated according to ICH guideline. Densitometric IH carried out the absorbance mode at 287 nm using ethyl acetate: 0.389 M ammonium acetate methanol (1:5, v/v) solvent system. This system found give compact spots Rf value 0.36 ± 0.01. Moreover, subjected...

A rapid and sensitive reversed-phase high-performance liquid chromatography (HPLC) method using novel salting-out assisted liquid-liquid extraction technique has been developed for the quantitative determination of febuxostat (FEB), used treatment gout, in rat plasma. The was validated according to US FDA guideline. Separation achieved a Phenomenex Luna-C18 (250 × 4.60 mm, 5 µm) column mobile phase composed potassium dihydrogen orthophosphate buffer 25 mM, adjusted pH 6.8 with...

The present study describes the development of a robust method for separation aspirin and simvastatin using reverse phase high performance liquid chromatographic on Kintex C18 column (5 μm, 250 mm × 4.6 mm) with UV detection at 234 nm. Box-Behnken design was applied multivariate optimization experimental conditions RP-HPLC obtaining desired resolution limited number experiments. Risk assessment performed to identify critical parameters. Three independent parameters; volume acetonitrile,...

Cholesterol is one of the essential intrauterine factors required for fetal growth and development. Maternal high cholesterol levels are known to be detrimental offspring health. However, its long-term effect on skeletal development remains elucidated. We performed our studies in two strains mice (C57BL6/J Swiss Albino) human subjects (65 mother-female newborn dyads) understand regulation by maternal cholesterol. found that from high-cholesterol-fed dams had low birth weight, smaller body...

K. N. Patela, R. S. Limgavkara, H. G. Ravala, Patela* & T. Gandhiaa Department of Quality Assurance, Anand Pharmacy College, Anand, Gujarat, India

A high performance liquid chromatographic method was developed and validated for the quantitative determination of brinzolamide brimonidinetartrate. Employing an isocratic RP-HPLC Phenomenex C18 (5 μm, 250×4.6 mm) column resulted in adequate separation brinzolamideand brimonidine tartrate with retention time 5.7±0.345 3.8±0.568 min, respectively. Best resolution achieved phosphate buffer (pH 6.6):acetonitrile:methanol (45:15:40) as mobile phase pumped at flow rate 1.0 ml/min detection...

Abstract Box‐Behnken design was applied for the development of a novel, precise, simple, and accurate high‐performance thin‐layer chromatography method simultaneous estimation mentioned drugs in formulation. Optimization performed using Quality by Design. Principles risk assessment followed application optimization chromatographic conditions to determine critical parameters: volume hexane, chamber saturation time, solvent front their effects on attributes: R f values both drugs. Optimized...

A specific stability indicating high-performance thin-layer chromatographic method for analysis of rifaximin both as a bulk drug and in formulations was developed validated. The employed HPTLC aluminium plates precoated with silica gel 60 F 254 the stationary phase. optimized mobile phase system consisted n-hexane : 2-propanol acetone ammonia (5 4.1 1, v/v/v/v), which gave compact spots at 0.59 ± 0.03. Rifaximin subjected to forced degradation studies order check specificity method....

A simple, rapid, and precise high-performance thin-layer chromatographic method was developed for quantitative estimation of luteolin apigenin in Premna mucronata Roxb., family Verbenaceae. Separation performed on silica gel 60 F 254 HPTLC plates using toluene : ethyl acetate formic acid (6 4 0.3) as mobile phase elution markers from extract. The determination carried out fluorescence mode densitometric absorbance-reflection at 366 nm both apigenin. methanolic extract found to contain 10.2...

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