A5025425343- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Genetic Associations and Epidemiology
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Machine Learning in Bioinformatics
- Animal Genetics and Reproduction
- DNA and Nucleic Acid Chemistry
- Nutrition, Genetics, and Disease
- Genomics and Phylogenetic Studies
- RNA Research and Splicing
- Genetics, Bioinformatics, and Biomedical Research
- Bioinformatics and Genomic Networks
- Plant Molecular Biology Research
- Cancer Genomics and Diagnostics
- Genomics and Rare Diseases
- CRISPR and Genetic Engineering
- Circadian rhythm and melatonin
- Genetics and Neurodevelopmental Disorders
- Wheat and Barley Genetics and Pathology
- Stress Responses and Cortisol
- Hemoglobinopathies and Related Disorders
- Genetic Mapping and Diversity in Plants and Animals
- Peroxisome Proliferator-Activated Receptors
- Neuroendocrine regulation and behavior
Institute of Cytology and Genetics
2016-2025
Siberian Branch of the Russian Academy of Sciences
2014-2025
Kurchatov Institute
2024
Research Institute of Fundamental and Clinical Immunology
2021
Novosibirsk State University
1997-2020
Children's Hospital of Los Angeles
2016-2017
University of Southern California
2016-2017
Russian Academy of Sciences
2000-2016
Development Agency of Serbia
2014
Human genome sequencing has resulted in a great body of data, including stunningly large number single nucleotide polymorphisms (SNPs) with unknown phenotypic manifestations. Identification and comprehensive analysis regulatory SNPs human gene promoters will help quantify the effects these on health. Based our experimental computer-aided study TATA boxes use literature we have derived an equation for TBP/TATA equilibrium binding three successive steps: TATA-binding protein (TBP) sliding...
Abstract MOTIVATION: A reliable recognition of transcription factor binding sites is essential for analysis regulatory genomic sequences. The experimental data make evident an important role DNA conformational features site functioning. However, Internet-available tools revealing and physicochemical significant the functioning subsequent use these have not been developed up to now. RESULTS: We suggest approach properties functional implemented in database B-DNA-VIDEO. This designed study...
SNPs in ТАТА boxes are the cause of monogenic diseases, contribute to a large number complex and have implications for human sensitivity external internal environmental signals. The aim this work was explore kinetic characteristics formation ТВР complexes with boxes, which associated β‑thalassemias diverse severity, immunosuppression, neurological disorders, so on. It has first time been demonstrated, using an electrophoretic mobility shift assay, that TBP interacts SNP-containing...
The use of biomedical SNP markers diseases can improve effectiveness treatment. Genotyping patients with subsequent searching for SNPs more frequent than in norm is the only commonly accepted method identification within framework translational research. bioinformatics applications aimed at millions unannotated “1000 Genomes” make this search focused and less expensive. We used our Web service involving Fisher’s<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"...
In this work, we for the first time performed a comprehensive bioinformatics analysis of 568 human genes that, according to NCBI Gene database as on September 15, 2024, were associated with pain generation, perception and anesthesia. The SCN9A gene encoding sodium voltage-gated channel α subunit 9 expressed in sensory neurons transferring signals central nervous system about tissue damage was only one involved all processes interest at once hub gene. First, our tool called OrthoWeb,...
Data on the genetics and molecular biology of diabetes are accumulating rapidly. This poses challenge creating research tools for a rapid search for, structuring analysis information in this field. We have developed web resource, GlucoGenes ® , which includes database an Internet portal genes proteins associated with high glucose (hyperglycemia), low (hypoglycemia), both metabolic disorders. The data were collected using text mining publications indexed PubMed Central gene networks...
The fundamental understanding of many biological processes that unfold in a human body has become possible due to experimental studies on animal models. backbone modern biomedical research is the use mouse models for studying important pathophysiological mechanisms, assessing new therapeutic approaches and making decisions acceptance or rejection candidate medicines preclinical trials. mice advantageous because they have small size, are easy keep genetically modify. Mice make up more than 90...
Obesity affects quality of life and expectancy is associated with cardiovascular disorders, cancer, diabetes, reproductive disorders in women, prostate diseases men, congenital anomalies children. The use single nucleotide polymorphism (SNP) markers drug responses (i.e., significant differences personal genomes patients from the reference human genome) can help physicians to improve treatment. Clinical research validate SNP via genotyping demonstration that alleles are significantly more...
Abstract MOTIVATION: The commonly accepted statistical mechanical theory is now multiply confirmed by using the weight matrix methods successfully recognizing DNA sites binding regulatory proteins in prokaryotes. Nevertheless, recent evaluation of application for transcription factor site recognition eukaryotes has unexpectedly revealed that scores correlate better to each other than activity interacting with proteins. This observation points out molecular mechanisms DNA/protein are more...
Although half of hypertensive patients have parents, known hypertension-related human loci identified by genome-wide analysis explain only 3% hypertension heredity. Therefore, mainstream transcriptome profiling subjects addresses differentially expressed genes (DEGs) specific to gender, age, and comorbidities in accordance with predictive preventive personalized participatory medicine treating according their symptoms, individual lifestyle, genetic background. Within this paradigm, here, we...
Aggressiveness in humans is a hereditary behavioral trait that mobilizes all systems of the body—first all, nervous and endocrine systems, then respiratory, vascular, muscular, others—e.g., for defense oneself, children, family, shelter, territory, other possessions as well personal interests. The level aggressiveness person determines many characteristics quality life lifespan, acting stress factor. Aggressive behavior depends on parameters such age, gender, diseases treatment, diet,...
Some variations of human genome [for example, single nucleotide polymorphisms (SNPs)] are markers hereditary diseases and drug responses. Analysis them can help to improve treatment. Computer-based analysis millions SNPs in the 1000 Genomes project makes a search for SNP more targeted. Here, we combined two computer-based approaches: DNA sequence keyword databases. In binding sites TATA-binding protein (TBP) gene promoters, found candidate gender-biased autoimmune diseases, including...
The progress of medicine, science, technology, education, and culture improves, year by year, quality life expectancy the populace. modern human has a chance to further improve duration his/her lives loved ones bringing their lifestyle in line with sequenced individual genomes. With this mind, one genome-based developments at junction personalized medicine bioinformatics will be considered work, where we used two Web services: (i) SNP_TATA_Comparator search for alleles single nucleotide...
Synthetic targeted optimization of plant promoters is becoming a part progress in mainstream postgenomic agriculture along with hybridization cultivated plants wild congeners, as well marker-assisted breeding. Therefore, here, for the first time, we compiled all experimental data—on mutational effects proximal on gene expression—that could find PubMed. Some these datasets cast doubt both existence and uniqueness sought solution, which unequivocally estimate promoter mutation expression when...
Single base mutations G→A at position 663 and G→T 666 of intron 6 the human tryptophan oxygenase gene ( TDO2 ) are associated with a variety psychiatric disorders [Comings, D.E. et al. (1996) Pharmacogenetics 6, 307–318]. Binding rat liver nuclear extract proteins to synthetic double‐strand oligonucleotides corresponding three allelic states region between 651 bp 680 has been studied by gel shift assay. It demonstrated that each state there corresponds specific set interacts it. With aid...
Recognition of functional sites remains a key event in the course genomic DNA annotation. It is well known that number have their own specific oligonucleotide content. This pinpoints fact preference site-specific nucleotide combinations at adjacent positions within an analyzed site could be informative for this recognition. Hence, Web-available resources describing content and applying above approach recognition are needed. However, they been poorly developed up to now.To describe sites, we...
We have used a stepwise increase in ligand complexity approach to estimate the relative contributions of nucleotide units DNA containing 7,8-dihydro-8-oxoguanine (oxoG) its total affinity for human 8-oxoguanine glycosylase (OGG1) and construct thermodynamic models enzyme interaction with cognate non-cognate DNA. Non-specific OGG1 interactions 10–13 nt pairs within DNA-binding cleft provides approximately 5 orders magnitude (ΔG° −6.7 kcal/mol). The contribution oxoG unit −3.3 kcal/mol)...
While year after year, conditions, quality, and duration of human lives have been improving due to the progress in science, technology, education, medicine, only eight diseases increasing prevalence shortening because premature deaths according retrospective official review on state US health, 1990-2010. These are kidney cancer, chronic diseases, liver diabetes, drug addiction, poisoning cases, consequences falls, Alzheimer's disease (AD) as one leading pathologies. There familial AD...
Plant hormone auxin is a key regulator of growth and development. Auxin affects gene expression through ARF transcription factors, which bind specifically responsive elements (AuxREs). genes usually have more than one AuxRE, for example, widely used sensor DR5 contains seven AuxREs. regions several plant been studied using sets transgenic constructions in the activity or AuxREs were abolished. Here we present method analysis datasets on promoter assays having sequences, namely, number...