A5010764903- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Vibrio bacteria research studies
- Essential Oils and Antimicrobial Activity
- Bacterial biofilms and quorum sensing
- Enterobacteriaceae and Cronobacter Research
- Viral gastroenteritis research and epidemiology
- Bee Products Chemical Analysis
- Nosocomial Infections in ICU
- Antibiotic Use and Resistance
- Healthcare Regulation
- Pharmaceutical and Antibiotic Environmental Impacts
- Banana Cultivation and Research
- Wound Healing and Treatments
- Complement system in diseases
- Salmonella and Campylobacter epidemiology
- Pneumonia and Respiratory Infections
- Phytochemicals and Medicinal Plants
- Seed and Plant Biochemistry
- Heavy Metals in Plants
- Growth and nutrition in plants
- Coconut Research and Applications
- Moringa oleifera research and applications
- Dental Erosion and Treatment
- Lipoproteins and Cardiovascular Health
Universidade Federal do Maranhão
2016-2025
Centro Universitário do Maranhão
2017-2024
Instituto Butantan
2012-2016
Staphylococcus aureus is one of the most common bacterial isolates found in wounds. Thus, innovative dressings, such as hydrogels, are interesting vehicles for incorporating bioactive compounds like those from Melaleuca alternifolia essential oil (MaEO). In this study, we evaluated antimicrobial and anti-inflammatory potential MaEO incorporated into an alginate chitosan hydrogel treating wounds infected by S. aureus. The with 1% (HMa 1%) was homogeneous a bright pale-yellow color...
Escherichia coli is responsible for cases of diarrhea around the world, and some studies have shown benefits cinnamaldehyde in treatment bacterial disease. Therefore, objective this study was to evaluate effects mice colonized by pathogenic E. coli, as well provide more insights into its antimicrobial action mechanism. After determination minimum inhibitory (MIC) bactericidal (MBC) concentrations, interference macromolecular pathways (synthesis DNA, RNA, protein, cell wall) measured...
Enteroaggregative and uropathogenic Escherichia coli, Shigella flexneri 2a, the hybrid enteroaggregative/Shiga toxin-producing E. coli strain (O104:H4) are important pathogens responsible for intestinal urinary tract infections, as well sepsis hemolytic uremic syndrome. They have in common production of a serine protease called Pic. Several biological roles Pic been described, including protection DH5α from complement-mediated killing. Hereby we showed that significantly reduces complement...
Autotransporter (AT) protein-encoding genes of diarrheagenic Escherichia coli (DEC) pathotypes (cah, eatA, ehaABCDJ, espC, espI, espP, pet, pic, sat, and tibA) were detected in typical atypical enteropathogenic E. (EPEC) frequencies between 0.8% 39.3%. Although these ATs have been described particular DEC pathotypes, their presence EPEC indicates that they should not be considered specific virulence markers.
Autotransporter proteins (AT) are associated with bacterial virulence attributes. Originally identified in enteroaggregative Escherichia coli (EAEC), Shigella flexneri 2a and uropathogenic E. coli, the serine protease Pic is one of these AT. We have previously detected atypical enteropathogenic strain (BA589) carrying pic gene. In present study, we characterized biological activities produced by BA589 both vitro vivo. Contrarily to other Pic-producers bacteria, located on a high molecular...
Chenopodium ambrosioides L. (Amaranthaceae) is often used in different kinds of vegetal preparations for medicinal purposes many clinical situations. Some studies have demonstrated its anti-inflammatory and immunomodulatory properties. The aim this work was to investigate the effect prophylactic treatment with hydroalcoholic extract C. (HCE) hexanic fraction (HEX) on control bacterial growth, activation phagocytes systemic inflammatory response a sepsis experimental model. Animals were...
Leptospires are highly motile spirochetes equipped with strategies for efficient invasion and dissemination within the host. Our group previously demonstrated that pathogenic leptospires secrete proteases capable of cleaving inactivating key molecules complement system, allowing these bacteria to circumvent host´s innate immune defense mechanisms. Given successful during infection, we wondered if such would target a broader range host molecules. In present study, proteolytic activity...
Staphylococcus aureus is a notorious human pathogen associated with serious nosocomial and community-acquired infections, such as pneumonia, meningitis, endocarditis, toxic shock syndrome, sepsis, among others. The objective of this study was to investigate the molecular profile, antimicrobial resistance, clonal diversity S. isolated from bloodstream. determination minimum inhibitory concentration (MIC) performed by an automated method. presence several virulence resistance genes evaluated...
The relevance of oxidative stress in the pathogenesis several diseases (including inflammatory disorders) has traditionally led to search for new sources antioxidant compounds. In this work, we report selection fractions with high action from B. tetraphylla (BT) leaf extracts. vitro methods (DPPH and ABTS assays; determination phenolic flavonoid contents) were used select products derived action. Then, samples highest potentials evaluated a model injury based on inoculation lethal dose...
INTRODUCTION: Extended spectrum β-lactamases (ESBLs) are enzymes that degrade β-lactam antibiotics and have been reported to be an important cause of nosocomial infection in worldwide. METHODS: During 2009, 659 enterobacteria strains were isolated from different clinical specimens tested for ESBL production. The disk approximation test, combined method addition clavulanic acid used phenotypic detection the ESBL-producing PCR blaTEM blaCTX-M genes. RESULTS: Among isolates, 125 producers....
The number of ESBL-producing Enterobacteriaceae in community-acquired urinary tract infections worldwide is probably underestimated because the technical difficulties encountered with their detection. In this study, out 5,672 urine samples analyzed, 916 were positive for uropathogens, 472 them being enterobacteria which 7.6% produced β-lactamases. Analysis isolated from 36 patients showed a high level antibiotic resistance, 52.7% and 80.5% isolates expressing blaTEM blaCTX-M, respectively.
Chronic use of statins may have anti-inflammatory action, promoting immunomodulation and survival in patients with sepsis. This study aimed to analyze the effects pretreatment simvastatin lethal sepsis induced by cecal ligation puncture (CLP). Male Swiss mice received prophylactic treatment or pyrogen-free water orally a single daily dose for 30 days. After this period, CLP was performed. Naïve Sham groups were performed as non-infected controls. Animal monitored 60 hours after CLP. Half...
Several natural products have been investigated for their bactericidal potential, among these, cinnamaldehyde. In this study, we aimed to evaluate the activity of cinnamaldehyde in treatment animals with sepsis induced by extraintestinal pathogenic E. coli. Initially, coli F5 was incubated minimum inhibitory and concentration. Animal survival monitored five days, a subset mice were euthanized after 10 h histological, hematological, immunological parameters, as well presence bacteria organs....
The incidence of infections caused by rapidly growing mycobacteria (RGM), especially Mycobacterium abscessus subsp. massiliense ( Mabs ), is increasing worldwide. Severe are associated with abscess formation and strong inflammatory response. This study evaluated the antimicrobial anti-inflammatory activities a hydroalcoholic extract (BoHE) ethyl acetate fraction (BoEA) Bixa orellana leaves. Antimicrobial activity was broth microdilution to determine minimum inhibitory (MIC) bactericidal...
In folk medicine, Vismia guianensis is used to treat skin diseases and mycoses in the Amazon region. We evaluated anti-Candida activity of hydroalcoholic extract from leaves (EHVG). HPLC-PDA FIA-ESI-IT-MSn were chemically characterize EHVG. The was determined vitro by minimum inhibitory concentrations (MIC) against Candida glabrata (ATCC-2001); albicans (ATCC-90028, ATCC-14053, ATCC-SC5314), C. clinical isolates. EHVG effects on adhesion, growth, biofilm formation also determined. Molecular...
Escherichia coli is an important pathogen responsible for a variety of diseases. We have recently shown that Pic, serine protease secreted by E. coli, mediates immune evasion the direct cleavage complement molecules. The aim this study was to investigate action Pic-producing bacteria in murine model sepsis. Mice were infected with (F5) or F5∆pic mutant. Animal survival monitored five days, and subset mice euthanized after 12 h sample acquisition. inoculation induced 100% death within 24 h....
Healthcare-related infections caused by resistant microorganisms are a severe public health problem and becoming increasingly prevalent in the hospital environment, especially Pseudomonas aeruginosa. This work aimed to evaluate resistance profile of aeruginosa antimicrobials before COVID-19 pandemic during period. Bacteria strains were obtained from tracheal aspiration, sputum, bronchoalveolar lavage for diagnosis phenotypic characterization. Matrix assisted laser-desorption ionization-time...
Acquired metallo-β-lactamases (MβL) are emerging determinants of resistance in Pseudomonas aeruginosa and Acinetobacter baumannii. The objectives this study were to phenotypically detect MβL imipenem-resistant P. A. baumannii, investigate the association between MβL-positive strains hospitals, compare profiles MβL-producing non-MβL-producing strains.The approximation disk combined assay methods used study.A total 18 (38.3%) isolates 1 (5.6%) baumannii isolate tested positive for presence...
A pneumonia é uma doença caracterizada pela inflamação do parênquima pulmonar devido à infecção causada por diversos tipos de microrganismos patogênicos. Vários são os pneumonia, dentre quais podemos destacar a Pneumonia Associada Ventilação (PAV), considerada como que surge em ambiente hospitalar entre 48 72 h após intubação oro traqueal e instituição ventilação mecânica invasiva sua incidência varia amplamente 5 40%. Desta forma, o objetivo deste estudo foi avaliar as causas da Mecânica...
A pneumonia é uma doença infecciosa que atinge os alvéolos pulmonares, ocasionada, principalmente, por bactérias, vírus, fungos e parasitas. Dentre as principais Haemophilus influenzae, Klebsiella pneumonia, Streptococcus pneumoniae, Staphylococcus aureus do grupo das Gram-negativas não fermentadoras de glicose, a exemplo Acinetobacter spp. Pseudomonas aeruginosa, são mais isoladas. Estima-se 450 milhões casos ocorram ano em todo o mundo Brasil protagonista na lista dos países com maior...