A5006674380- Receptor Mechanisms and Signaling
- Cellular transport and secretion
- Toxin Mechanisms and Immunotoxins
- Lipid Membrane Structure and Behavior
- Lipid metabolism and biosynthesis
- Sports injuries and prevention
- Endoplasmic Reticulum Stress and Disease
- Sports Performance and Training
- Photosynthetic Processes and Mechanisms
- Surface Modification and Superhydrophobicity
- Transgenic Plants and Applications
- Particle accelerators and beam dynamics
- Microtubule and mitosis dynamics
- Magnetic Properties of Alloys
- ATP Synthase and ATPases Research
- Pancreatic and Hepatic Oncology Research
- Neuroscience and Neuropharmacology Research
- Immunotherapy and Immune Responses
- Ubiquitin and proteasome pathways
- Offshore Engineering and Technologies
- Oil and Gas Production Techniques
- Sport Psychology and Performance
- Neurological Disease Mechanisms and Treatments
- Petroleum Processing and Analysis
- Icing and De-icing Technologies
Beijing Sport University
2020-2023
Shantou University
2022-2023
Shantou University Medical College
2022-2023
Kunming Medical University
2019-2023
Shenzhen University Health Science Center
2016-2021
China National Petroleum Corporation (China)
1999-2021
Shenzhen University
2020
State Key Laboratory of Respiratory Disease
2020
Augusta University
2011-2019
Chinese University of Hong Kong
2008-2018
Peripheral and integral membrane proteins can be located in several different subcellular compartments, it is often necessary to determine the location of such or track their movement living cells. Image-based colocalization labeled compartment markers frequently used for this purpose, but method limited terms throughput resolution. Here we show that bioluminescence resonance energy transfer (BRET) between interest compartment-targeted BRET partners report live The sensitivity sufficient...
Abstract Bioluminescence resonance energy transfer (BRET) is often used to study association of membrane proteins and in particular oligomerization G protein-coupled receptors (GPCRs). Oligomerization class A GPCRs controversial, part because the methods this question are not completely understood. Here we reconsider β 2 adrenergic receptor (β AR) reevaluate BRET titration as a method protein association. Using inducible expression acceptor at multiple levels donor find that between AR...
G-protein-coupled receptors (GPCRs) constitute the largest superfamily of cell-surface signaling proteins. However, mechanisms underlying their surface targeting and sorting are poorly understood. Here, we screen Rab family small GTPases in transport multiple GPCRs. We find that manipulation Rab43 function significantly alters presentation all GPCRs studied without affecting non-GPCR membrane specifically regulates nascent from endoplasmic reticulum (ER) to Golgi. More interestingly,...
Highlights•Six TBC proteins activity-dependently regulate the cell surface export of GPCRs•TBC selectively control ER-Golgi or Golgi-cell transport GPCRs•TBC1D6 is a GAP for Rab26 and physically associates with Rab26•TBC1D6 modulates interaction post-Golgi traffic GPCRsSummaryG-protein-coupled receptors (GPCRs) constitute largest superfamily signaling proteins. However, molecular mechanisms underlying their delivery after synthesis remain poorly understood. Here, we screen domain-containing...
Magnetic polymers show great potential in biomedical, electronic devices and magnetic responsive materials. The structure property relationship are very important design of organic polymers. In this work,...
Neochamaejasmin A (1), a biflavonoid isolated from the roots of traditional Chinese medicine, Stellera chamaejasme L., was shown to inhibit cellular 3H-thymidine incorporation (IC50 12.5 µg/mL) and subsequent proliferation human prostate cancer LNCaP cells. Treatment cells with low doses 1 (≤6.25 suppressed DNA-binding activities transcription factors NFκB AP-1 promoter cyclin D also inhibited expression cell cycle regulatory proteins D, proliferating nuclear antigen, nucleolin, thus...
Molecular mechanisms governing the anterograde trafficking of nascent G protein-coupled receptors (GPCRs) are poorly understood. Here, we have studied regulation cell surface transport α2-adrenergic (α2-ARs) by GGA3 (Golgi-localized, γ-adaptin ear domain homology, ADP ribosylation factor-binding protein 3), a multidomain clathrin adaptor that sorts cargo proteins at trans-Golgi network (TGN) to endosome/lysosome pathway. By using an inducible system, demonstrated knockdown significantly...
Video assistant referee (VAR) has been implemented in women's football, aiming to improve referees' decision-making, but its impact not yet analyzed. This study intended explore how the VAR affects refereeing decisions at Fédération Internationale de Football Association (FIFA) Women's World Cup competitions. The sample includes all 52 matches played 2015 tournament before was introduced and 2019 competition where deployed. For each match, data on ten variables were collected: first half...
Trichosanthin (TCS) is a type I ribosome-inactivating protein extracted from the tuberous root of plant Trichosanthes. TCS shows promising potential in clinical drug abortion, anti-tumor and immunological regulation. However, molecular mechanisms its immune regulation properties are still not well discovered. In present study, we investigated activity hepatocellular carcinoma (HCC), both vitro vivo. Both HCC cell lines xenograft tumor tissues showed considerable growth inhibition after they...
// Chunman Li 1 , Meiqi Zeng Huju Chi Jing Shen 2 Tzi-Bun Ng 3 Guangyi Jin 4 Desheng Lu Xinmin Fan Bilian Xiong Zhangang Xiao Ou Sha Department of Anatomy, Histology and Developmental Biology, School Basic Medical Sciences, Shenzhen University Health Science Centre, Shenzhen, Guangdong, China Laboratory Molecular Pharmacology, Pharmacy, Southwest University, Luzhou, Sichuan, Biomedical Faculty Medicine, The Chinese Hong Kong, Correspondence to: Sha, email: shaou@szu.edu.cn Xiao,...
Geoditin A, an isomalabaricane triterpene isolated from the marine sponge Geodia japonica, has been demonstrated to dissipate mitochondrial membrane potential, activate caspase 3, decrease cytoplasmic proliferating cell nuclear antigen (PCNA), and induce apoptosis of leukemia cells, but underlying mechanism remains unclear [1]. In this study, we found fragmentation Golgi structure, suppression transferrin receptor expression, production oxidants, DNA in human colon cancer HT29 cells after...
Lipid droplet (LD) homeostasis involves activities of various RAB small GTPases. Recently, we found RAB40C was one the proteins regulating LD homeostasis. contains a unique SOCS domain that is required for clustering LDs. However, its precise functional role in and mechanism regulation remain largely unknown. In this study, observed over-accumulation LDs cells with deleted by Crispr-Cas9 editing. appeared to reduce accumulation after long term incubation oleic acid (24 hours). Unexpectedly,...
Background: DL-3-n-butylphthalide (NBP) is a drug for treating acute ischemic stroke, and may play neuroprotective role by acting on multiple active targets. The aim of this study was to predict the target proteins NBP in mammalian cells. Methods: similarity ensemble approach search tool (SEArch), one commonly used public bioinformatics tools prediction, employed experiment. molecular docking performed using three-dimensional (3-D) crystal structure, substrate free. software AutoDock Vina...
Youth football training should ensure proper player development. However, coaches worldwide behave differently during their coaching process. Thus, this study aims to: a) compare the behaviour and activities of from China Spain, b) explore impact different pedagogy on coach's players' perceptions. Systematic observations semi-structured interviews were developed for 6 youth Madrid (n = 3) Beijing 3). And 16 players randomly selected each academy queried perception. The results showed...
The transport protein particle (TRAPP) complex was initially identified as a tethering factor for COPII vesicle. Subsequently, three forms (TRAPPI, II, and III) have been found TRAPPIII has reported to serve regulator in autophagy. This study investigates new role of mammalian ciliogenesis. We ciliopathy protein, oral-facial-digital syndrome 1 (OFD1), interacting with the TRAPPIII-specific subunits TRAPPC8 TRAPPC12. is necessary association OFD1 pericentriolar material (PCM1). Its depletion...