A5005166932- Tuberculosis Research and Epidemiology
- Bacteriophages and microbial interactions
- Mycobacterium research and diagnosis
- SARS-CoV-2 and COVID-19 Research
- RNA and protein synthesis mechanisms
- SARS-CoV-2 detection and testing
- Cancer Genomics and Diagnostics
- CRISPR and Genetic Engineering
- Biosensors and Analytical Detection
- Genetically Modified Organisms Research
- Neutropenia and Cancer Infections
- Insect Resistance and Genetics
- Diagnosis and treatment of tuberculosis
- Microbial Community Ecology and Physiology
- Influenza Virus Research Studies
- Lung Cancer Treatments and Mutations
- Advanced biosensing and bioanalysis techniques
- Genomics and Phylogenetic Studies
Sciensano (Belgium)
2020-2025
Similar to genetically modified organisms (GMOs) produced by classical genetic engineering, gene-edited (GE) and their derived food/feed products commercialized on the European Union market fall within scope of Directive 2001/18/EC. Consequently, control in chain GMO enforcement laboratories is required competent authorities guarantee safety traceability (2003/1829/EC; 2003/1830/EC). However, detection potentially challenging at both analytical interpretation levels since this requires...
The use of whole-genome sequencing (WGS) for routine typing bacterial isolates has increased substantially in recent years. For Mycobacterium tuberculosis (MTB), particular, WGS the benefit drastically reducing time required to generate results compared most conventional phenotypic methods. Consequently, a multitude solutions analyzing MTB data have been developed, but their successful integration clinical and national reference laboratories is hindered by requirement validation, which...
The influx of whole genome sequencing (WGS) data in the public health and clinical diagnostic sectors has created a need for analysis methods bioinformatics expertise, which can be bottleneck many laboratories. At Sciensano, Belgian national institute, an intuitive user-friendly tool portal was implemented using Galaxy, open-source platform workflow creation. Galaxy @Sciensano instance is available to both internal external scientists offers wide range tools provided by community,...
The ongoing COVID-19 pandemic, caused by SARS-CoV-2, constitutes a tremendous global health issue. Continuous monitoring of the virus has become cornerstone to make rational decisions on implementing societal and sanitary measures curtail spread. Additionally, emerging SARS-CoV-2 variants have increased need for genomic surveillance detect particular strains because their potentially transmissibility, pathogenicity immune escape. Targeted sequencing diagnostic wastewater samples been...
Since January 2019, the Belgian National Reference Center for Mycobacteria (NRC) has switched from conventional typing to prospective whole-genome sequencing (WGS) of all submitted Mycobacterium tuberculosis complex (MTB) isolates. The ISO17025 validated procedure starts with semi-automated extraction and purification gDNA directly MGIT tubes, without preceding subculturing. All samples are then sequenced on an Illumina MiSeq sequencer analyzed using in-house developed bioinformatics...
Next-generation sequencing (NGS) is being integrated into routine clinical practice in the field of (hemato-) oncology to search for variants with diagnostic, prognostic, or therapeutic value at potentially low allelic frequencies. The complex workflows used require careful validation and continuous quality control. Participation external assessments (EQA) helps laboratories evaluate their performance guarantee validity tests results ultimate goal ensuring high-quality patient care. Here, we...
Influenza viruses exhibit considerable diversity between hosts. Additionally, different quasispecies can be found within the same host. High-throughput sequencing technologies used to sequence a patient-derived virus population at sufficient depths identify low-frequency variants (LFV) present in quasispecies, but many challenges remain for reliable LFV detection because of experimental errors introduced during sample preparation and sequencing. High genomic copy numbers extensive are...
Abstract The ongoing COVID-19 pandemic, caused by SARS-CoV-2, constitutes a tremendous global health issue. Continuous monitoring of the virus has become cornerstone to make rational decisions on implementing societal and sanitary measures curtail spread. Additionally, emerging SARS-CoV-2 variants have increased need for genomic surveillance detect particular strains because their potentially transmissibility, pathogenicity immune escape. Targeted sequencing wastewater been explored as an...