A5031292845- Viral Infections and Immunology Research
- Hepatitis C virus research
- SARS-CoV-2 and COVID-19 Research
- Immunodeficiency and Autoimmune Disorders
- Viral gastroenteritis research and epidemiology
- Hepatitis B Virus Studies
- RNA Research and Splicing
- T-cell and Retrovirus Studies
- Viral Infections and Outbreaks Research
- Bacteriophages and microbial interactions
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Animal Virus Infections Studies
- MicroRNA in disease regulation
- Virology and Viral Diseases
- COVID-19 Clinical Research Studies
- SARS-CoV-2 detection and testing
- Biochemical and Molecular Research
- RNA and protein synthesis mechanisms
- Herpesvirus Infections and Treatments
- Monoclonal and Polyclonal Antibodies Research
- RNA regulation and disease
Indian Institute of Science Bangalore
2017-2023
Direct massively parallel sequencing of SARS-CoV-2 genome was undertaken from nasopharyngeal and oropharyngeal swab samples infected individuals in Eastern India. Seven the isolates belonged to A2a clade, while one B4 clade. Specific mutations, characteristic were also detected, which included P323L RNA-dependent RNA polymerase D614G Spike glycoprotein. Further, our data revealed emergence novel subclones harbouring nonsynonymous viz. G1124V (S) protein, R203K, G204R nucleocapsid (N)...
Host protein HuR translocation from nucleus to cytoplasm following infection is crucial for the life cycle of several RNA viruses including hepatitis C virus (HCV), a major causative agent hepatocellular carcinoma. assists assembly replication-complex on viral-3'UTR, and its depletion hampers viral replication. Although cytoplasmic HCV replication, little known about how orchestrates mobilization into nucleus. We show that two proteins, NS3 NS5A, act co-ordinately alter equilibrium...
Coxsackievirus B3 is an enterovirus, with positive-sense single-stranded RNA genome containing 'Internal Ribosome Entry Site' (IRES) in the 5ʹUTR. Once sufficient viral proteins are synthesized cell from input RNA, template switches translation to replication synthesize negative-strand RNA. Inhibition of a key step regulating this switch as positive-strand should be free ribosomes enable polymerase movement. In study, we show how host protein hnRNP C1/C2 inhibits translation. interacts...
Since its origin in late 2019, the SARS-CoV-2 virus has been constantly mutating and evolving. Current studies mostly employ spike protein (S) pseudovirus systems to determine effects of mutations on infectivity immunogenicity variants.
During enteroviral infections, the canonical translation factor eukaryotic initiation 4 γ I (eIF4GI) is cleaved by viral protease 2A. The resulting C-terminal fragment recruited internal ribosome entry site (IRES) for efficient of RNA. However, 2A not present in capsid and synthesized only after initial round translation. This presents conundrum how occurs absence eIF4GI fragment. Interestingly, host protein DAP5 (also known as p97, eIF4GIII, eIF4G2), an isoform eIF4GI, closely resembles...
Abstract Host protein HuR translocation from nucleus to cytoplasm following infection is crucial for the life cycle of several RNA viruses including hepatitis C virus (HCV), a major causative agent hepatocellular carcinoma. assists assembly replication-complex on viral-3′UTR, and its depletion hampers viral replication. Although cytoplasmic HCV replication, little known about how orchestrates mobilization into nucleus. We show that two proteins, NS3 NS5A, act co-ordinately alter equilibrium...
Abstract Severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) viral RNA associates with different RNA-binding host proteins at each stage of its life cycle. We found sequence dependent binding one such important protein, human antigen R (HuR) to SARS-CoV-2 5′UTR and studied potential role in virus The knockdown knockout studies revealed importance translation. identified 5′-UTR mutations variants concern that altered the HuR-binding affinity. Interestingly, HuR enhanced...
Abstract The emergence and evolution of SARS-CoV-2 is characterized by the occurrence diverse sets mutations that affect virus characteristics, including transmissibility antigenicity. Recent studies have focused mostly on Spike protein mutations; however, variants interest (VoI) or concern (VoC) contain significant in nucleocapsid as well. To study relevance at virion level, recombinant baculovirus expression system based VLPs were generated for prototype Wuhan sequence along with mutants...