A5048565805- Metalloenzymes and iron-sulfur proteins
- Plant nutrient uptake and metabolism
- Legume Nitrogen Fixing Symbiosis
- Plant Micronutrient Interactions and Effects
- Photosynthetic Processes and Mechanisms
- Algal biology and biofuel production
- Electrocatalysts for Energy Conversion
- Cassava research and cyanide
- Aquatic Ecosystems and Phytoplankton Dynamics
- Plant Stress Responses and Tolerance
- Protist diversity and phylogeny
- Amino Acid Enzymes and Metabolism
- Ammonia Synthesis and Nitrogen Reduction
- Marine and coastal ecosystems
- Plant-Microbe Interactions and Immunity
- Chromosomal and Genetic Variations
- Pancreatic function and diabetes
- RNA modifications and cancer
- Folate and B Vitamins Research
- Nitrogen and Sulfur Effects on Brassica
- Metal-Catalyzed Oxygenation Mechanisms
- Phosphorus and nutrient management
- Nitric Oxide and Endothelin Effects
- Micro and Nano Robotics
University of Córdoba
2006-2023
Universidad Politécnica de Madrid
2015-2020
Centre for Plant Biotechnology and Genomics
2015-2020
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2016
University of Cologne
2014
In-Q-Tel
2011
Technische Universität Braunschweig
2006
Abstract Iron is critical for symbiotic nitrogen fixation (SNF) as a key component of multiple ferroproteins involved in this biological process. In the model legume Medicago truncatula, iron delivered by vasculature to infection/maturation zone (zone II) nodule, where it released apoplast. From there, plasma membrane transporters move into rhizobia-containing cells, used cofactor plant and rhizobial proteins (e.g. leghemoglobin bacterial nitrogenase). MtNramp1 (Medtr3g088460) M. truncatula...
Molybdenum is an essential element for almost all living beings, which, in the form of a molybdopterin-cofactor, participates active site enzymes involved key reactions carbon, nitrogen, and sulfur metabolism. This metal taken up by cells oxyanion molybdate. Bacteria acquire molybdate ATP-binding-cassette (ABC) transport system widely studied process, but how eukaryotic take molybdenum unknown because transporters have not been identified so far. Here, we report high-affinity transporter,...
Almost all living organisms need to obtain molybdenum from the external medium achieve essential processes for life. Activity of important enzymes such as sulfite oxidase, aldehyde xanthine dehydrogenase, and nitrate reductase is strictly dependent on presence Mo in its active site. Cells take up form oxianion molybdate, but molecular nature transporters still not well known eukaryotes. MOT1 first molybdate transporter identified plant-type eukaryotic organisms, it absent animal genomes....
Molybdenum, as a component of the iron-molybdenum cofactor nitrogenase, is essential for symbiotic nitrogen fixation. This nutrient has to be provided by host plant through molybdate transporters. Members transporter family Molybdate Transporter type 1 (MOT1) were identified in model legume Medicago truncatula and their expression nodules was determined. Yeast toxicity assays, confocal microscopy, phenotypical characterization Transposable Element from Nicotiana tabacum (Tnt1) insertional...
Iron (Fe) is an essential micronutrient for symbiotic nitrogen fixation in legume nodules, where it required the activity of bacterial nitrogenase, plant leghemoglobin, respiratory oxidases, and other Fe proteins both organisms. solubility transport within between tissues facilitated by organic chelators, such as nicotianamine citrate. We have characterized a nodule-specific citrate transporter multidrug toxic compound extrusion family, MtMATE67
Abstract Symbiotic nitrogen fixation in legume root nodules requires a steady supply of molybdenum for synthesis the iron‐molybdenum cofactor nitrogenase. This nutrient has to be provided by host plant from soil, crossing several symplastically disconnected compartments through molybdate transporters, including members MOT1 family. Medicago truncatula Molybdate Transporter (MtMOT) 1.2 is family member located endodermal cells roots and nodules. Immunolocalization tagged MtMOT1.2 indicates...
The molybdenum cofactor (Moco) forms the catalytic site in all eukaryotic enzymes and is synthesized by a multistep biosynthetic pathway. mechanism of transfer, storage, insertion Moco into appropriate apo-enzyme poorly understood. In Chlamydomonas reinhardtii, carrier protein (MCP) has been identified characterized recently. Here we show biochemical evidence that MCP binds as well tungstate-substituted form (Wco) with high affinity, whereas molybdopterin, ultimate precursor, not bound. This...
Zinc is a micronutrient required for symbiotic nitrogen fixation. It has been proposed that in model legume Medicago truncatula, zinc delivered by the root vasculature into nodule and released infection/differentiation zone. There, transporters must introduce this element rhizobia-infected cells to metallate apoproteins use as cofactor. MtZIP6 (Medtr4g083570) an M. truncatula Zinc-Iron Permease (ZIP) expressed only roots nodules, with highest expression levels Immunolocalization studies...
Summary Iron is an essential cofactor for symbiotic nitrogen fixation, required by many of the enzymes involved, including signal transduction proteins, O 2 homeostasis systems, and nitrogenase itself. Consequently, host plants have developed a transport network to deliver iron nitrogen‐fixing nodule cells. Ferroportin family members in model legume Medicago truncatula were identified their expression was determined. Yeast complementation assays, immunolocalization, characterization tnt1...
Microalgae are used in various biotechnological processes, such as biofuel production due to their high biomass yields, agriculture biofertilizers, of high-value-added products, de-contamination wastewater, or biological models for carbon sequestration. The number these applications is increasing, and such, any advances that contribute reducing costs increasing economic profitability can have a significant impact. Nitrogen fixing organisms, often called diazotroph, also great potential,...
Molybdenum (Mo) is a vital micronutrient for nearly all living organisms, serving as cofactor molybdoenzymes that catalyze essential redox reactions in nitrogen metabolism. Among these enzymes nitrate reductase plays crucial role assimilation. Maintaining Mo homeostasis—including uptake, storage, and utilization—is critical to avoid both deficiency toxicity. Our research focuses on uncovering novel molecular components involved homeostasis, particularly connection with...
Symbiotic nitrogen fixation carried out by the interaction between legumes and diazotrophic bacteria known as rhizobia requires relatively large levels of transition metals. These elements are cofactors many key enzymes involved in this process. Metallic micronutrients obtained from soil roots directed to sink organs vasculature, a process mediated number metal transporters small organic molecules that facilitate delivery plant fluids. Among later, nicotianamine is one most important....